Abstract: An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and/or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.

Abstract: System and method to improve drug delivery to identified regions in the brain or elsewhere through direct infusion of a therapeutic agent or the like into that region. This direct infusion will allow for greater concentrations of the agent in the target region while reducing concentrations elsewhere in the body where these agents may be toxic. The system and method improves efficacy while reducing unwanted side effects. The system includes an array of multiple, independently targeted, microporous catheters for insertion into the target region and a distribution system that allows for individualized flow control to each catheter. The system may be connected to a reservoir that contains the therapeutic agent, and flow to the system is maintained through one or more pumps. This system will greatly improve on the current single catheter infusion design and shall provide therapy, delivered through multiple catheters, thus delivering the therapy evenly over a customizable volume.

Abstract: A microfluidic device can include an upstream passage, a sample passage, a bifurcating passage, and a combining passage. The upstream passage can be configured to provide a focusing stream. The sample passage can be configured to provide a sample stream. The bifurcating passage can include a specified bifurcating flow resistance. The combining passage can be configured to create a combined stream from the focusing stream and the sample stream, where the focusing stream can direct the sample stream away from the upstream passage and toward the bifurcating passage. A first portion of the combined stream can be discharged through the bifurcating passage. The main discharge can be configured to discharge a second portion of the combined stream. The main discharge can include a main discharge resistance that is selectable to vary the main discharge resistance relative to the bifurcating flow resistance.

Abstract: Systems, methods, and devices are described herein for identifying, monitoring, isolating, or selecting a cell having a predefined characteristic in a mixed population of cells utilizing a combination of any one or more of iDEP, a region of localized field enhancement, a variable frequency electric field, a wide bandwidth amplifier, and/or an imaging apparatus.

Abstract: A microfluidic device can include a superstructure defining a microfluidic channel therein and a first hydrogel bonded to the microfluidic channel to define a perfusable channel therein, the first hydrogel including cells embedded therein or thereon. The microfluidic device can optionally include a second hydrogel bonded to the microfluidic channel or to the hydrogel.

Abstract: An exemplary method and system is disclosed that facilitate the integration of on-chip impedance sensors and measurement circuitries, e.g., in characterizing internal microfluidic structures and/or in cell or particle cytometry, for quantifying the impedance/frequency response of microfluidic device under the same, or similar, conditions used for particle manipulation. In some embodiments, the exemplary method and system employs a circuit configured for automated determination and quantification of parasitic voltage drops during AC electrokinetic particle manipulation, without the need to use valuable biological samples or model particles. The determined impedance response can be used to assess efficacy of the microfluidic device geometry as well as to provide control signals to inform downstream cell separation decisions.

Abstract: A microfluidic device can include an upstream passage, a sample passage, a bifurcating passage, and a combining passage. The upstream passage can be configured to provide a focusing stream. The sample passage can be configured to provide a sample stream. The bifurcating passage can include a specified bifurcating flow resistance. The combining passage can be configured to create a combined stream from the focusing stream and the sample stream, where the focusing stream can direct the sample stream away from the upstream passage and toward the bifurcating passage. A first portion of the combined stream can be discharged through the bifurcating passage. The main discharge can be configured to discharge a second portion of the combined stream. The main discharge can include a main discharge resistance that is selectable to vary the main discharge resistance relative to the bifurcating flow resistance.

Abstract: An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and/or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.

Abstract: System and method to improve drug delivery to identified regions in the brain or elsewhere through direct infusion of a therapeutic agent or the like into that region. This direct infusion will allow for greater concentrations of the agent in the target region while reducing concentrations elsewhere in the body where these agents may be toxic. The system and method improves efficacy while reducing unwanted side effects. The system includes an array of multiple, independently targeted, microporous catheters for insertion into the target region and a distribution system that allows for individualized flow control to each catheter. The system may be connected to a reservoir that contains the therapeutic agent, and flow to the system is maintained through one or more pumps. This system will greatly improve on the current single catheter infusion design and shall provide therapy, delivered through multiple catheters, thus delivering the therapy evenly over a customizable volume.