Patents by Inventor Watt W. Webb
Watt W. Webb has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20080227654Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonuelcotide primer at an active site.Type: ApplicationFiled: February 6, 2008Publication date: September 18, 2008Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Patent number: 7416844Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site.Type: GrantFiled: November 21, 2005Date of Patent: August 26, 2008Assignee: Cornell Research Foundation, Inc.Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Patent number: 7361466Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site.Type: GrantFiled: March 25, 2005Date of Patent: April 22, 2008Assignee: Cornell Research Foundation, Inc.Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Patent number: 7292742Abstract: The present invention is directed to a method and an apparatus for analysis of an analyte. The method involves providing a zero-mode waveguide which includes a cladding surrounding a core where the cladding is configured to preclude propagation of electromagnetic energy of a frequency less than a cutoff frequency longitudinally through the core of the zero-mode waveguide. The analyte is positioned in the core of the zero-mode waveguide and is then subjected, in the core of the zero-mode wave guide, to activating electromagnetic radiation of a frequency less than the cut-off frequency under conditions effective to permit analysis of the analyte in an effective observation volume which is more compact than if the analysis were carried out in the absence of the zero-mode waveguide.Type: GrantFiled: February 2, 2007Date of Patent: November 6, 2007Assignee: Cornell Research Foundation, Inc.Inventors: Michael J. Levene, Jonas Korlach, Stephen W. Turner, Harold G. Craighead, Watt W. Webb
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Patent number: 7181122Abstract: The present invention is directed to a method and an apparatus for analysis of an analyte. The method involves providing a zero-mode waveguide which includes a cladding surrounding a core where the cladding is configured to preclude propagation of electromagnetic energy of a frequency less than a cutoff frequency longitudinally through the core of the zero-mode waveguide. The analyte is positioned in the core of the zero-mode waveguide and is then subjected, in the core of the zero-mode waveguide, to activating electromagnetic radiation of a frequency less than the cut-off frequency under conditions effective to permit analysis of the analyte in an effective observation volume which is more compact than if the analysis were carried out in the absence of the zero-mode waveguide.Type: GrantFiled: December 20, 2005Date of Patent: February 20, 2007Assignee: Cornell Research Foundation, Inc.Inventors: Michael J. Levene, Jonas Korlach, Stephen W. Turner, Harold G. Craighead, Watt W. Webb
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Patent number: 7056676Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site.Type: GrantFiled: December 16, 2004Date of Patent: June 6, 2006Assignee: Cornell Research Foundation, Inc.Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Patent number: 7056661Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site.Type: GrantFiled: May 17, 2000Date of Patent: June 6, 2006Assignee: Cornell Research Foundation, Inc.Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Patent number: 7052847Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site.Type: GrantFiled: December 15, 2004Date of Patent: May 30, 2006Assignee: Cornell Research Foundation, Inc.Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Patent number: 7033764Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site.Type: GrantFiled: December 15, 2004Date of Patent: April 25, 2006Assignee: Cornell Research Foundation, Inc.Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Patent number: 7013054Abstract: The present invention is directed to a method and an apparatus for analysis of an analyte. The method involves providing a zero-mode waveguide which includes a cladding surrounding a core where the cladding is configured to preclude propagation of electromagnetic energy of a frequency less than a cutoff frequency longitudinally through the core of the zero-mode waveguide. The analyte is positioned in the core of the zero-mode waveguide and is then subjected, in the core of the zero-mode waveguide, to activating electromagnetic radiation of a frequency less than the cut-off frequency under conditions effective to permit analysis of the analyte in an effective observation volume which is more compact than if the analysis were carried out in the absence of the zero-mode waveguide.Type: GrantFiled: June 13, 2005Date of Patent: March 14, 2006Assignee: Cornell Research Foundation, Inc.Inventors: Michael J. Levene, Jonas Korlach, Stephen W. Turner, Harold G. Craighead, Watt W. Webb
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Patent number: 6917726Abstract: The present invention is directed to a method and an apparatus for analysis of an analyte. The method involves providing a zero-mode waveguide which includes a cladding surrounding a core where the cladding is configured to preclude propagation of electromagnetic energy of a frequency less than a cutoff frequency longitudinally through the core of the zero-mode waveguide. The analyte is positioned in the core of the zero-mode waveguide and is then subjected, in the core of the zero-mode waveguide, to activating electromagnetic radiation of a frequency less than the cut-off frequency under conditions effective to permit analysis of the analyte in an effective observation volume which is more compact than if the analysis were carried out in the absence of the zero-mode waveguide.Type: GrantFiled: September 27, 2002Date of Patent: July 12, 2005Assignee: Cornell Research Foundation, Inc.Inventors: Michael J. Levene, Jonas Korlach, Stephen W. Turner, Harold G. Craighead, Watt W. Webb
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Patent number: 6839586Abstract: The present invention is directed to a method of applying radiation through an optical fiber for detecting disease within a plant or animal or imaging a particular tissue of a plant or animal. In addition, fluorescence can be detected and localized within a subject by such application of radiation through an optical fiber. The radiation is effective to promote simultaneous multiphoton excitation. The optical fibers are used alone to examine internal regions of tissue, in conjunction with an optical biopsy needle to evaluate sub-surface tissue, or with an endoscope to evaluate tissue within body cavities.Type: GrantFiled: May 5, 2003Date of Patent: January 4, 2005Assignee: Cornell Research Foundation, Inc.Inventor: Watt W. Webb
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Publication number: 20030191397Abstract: The present invention is directed to a method of applying radiation through an optical fiber for detecting disease within a plant or animal or imaging a particular tissue of a plant or animal. In addition, fluorescence can be detected and localized within a subject by such application of radiation through an optical fiber. The radiation is effective to promote simultaneous multiphoton excitation. The optical fibers are used alone to examine internal regions of tissue, in conjunction with an optical biopsy needle to evaluate sub-surface tissue, or with an endoscope to evaluate tissue within body cavities.Type: ApplicationFiled: May 5, 2003Publication date: October 9, 2003Inventor: Watt W. Webb
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Publication number: 20030174992Abstract: The present invention is directed to a method and an apparatus for analysis of an analyte. The method involves providing a zero-mode waveguide which includes a cladding surrounding a core where the cladding is configured to preclude propagation of electromagnetic energy of a frequency less than a cutoff frequency longitudinally through the core of the zero-mode waveguide. The analyte is positioned in the core of the zero-mode waveguide and is then subjected, in the core of the zero-mode waveguide, to activating electromagnetic radiation of a frequency less than the cut-off frequency under conditions effective to permit analysis of the analyte in an effective observation volume which is more compact than if the analysis were carried out in the absence of the zero-mode waveguide.Type: ApplicationFiled: September 27, 2002Publication date: September 18, 2003Inventors: Michael J. Levene, Jonas Korlach, Stephen W. Turner, Harold G. Craighead, Watt W. Webb
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Patent number: 6580941Abstract: The present invention is directed to a method of applying radiation through an optical fiber for detecting disease within a plant or animal or imaging a particular tissue of a plant or animal. In addition, fluorescence can be detected and localized within a subject by such application of radiation through an optical fiber. The radiation is effective to promote simultaneous multiphoton excitation. The optical fibers are used alone to examine internal regions of tissue, in conjunction with an optical biopsy needle to evaluate sub-surface tissue, or with an endoscope to evaluate tissue within body cavities.Type: GrantFiled: February 7, 2001Date of Patent: June 17, 2003Assignee: Cornell Research Foundation, Inc.Inventor: Watt W. Webb
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Publication number: 20030092005Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site.Type: ApplicationFiled: September 15, 2001Publication date: May 15, 2003Inventors: Michael J. Levene, Daniel R. Larson, Watt W. Webb
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Publication number: 20030044781Abstract: The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid, i.e. the activity of a nucleic acid polymerizing enzyme on the template nucleic acid molecule to be sequenced is followed in real time. The sequence is deduced by identifying which base is being incorporated into the growing complementary strand of the target nucleic acid by the catalytic activity of the nucleic acid polymerizing enzyme at each step in the sequence of base additions. A polymerase on the target nucleic acid molecule complex is provided in a position suitable to move along the target nucleic acid molecule and extend the oligonucleotide primer at an active site.Type: ApplicationFiled: May 17, 2000Publication date: March 6, 2003Inventors: Jonas Korlach, Watt W. Webb, Michael Levene, Stephen Turner, Harold G. Craighead, Mathieu Foquet
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Publication number: 20030009104Abstract: The present invention is directed to a method of detecting a neurodegenerative disease in a mammal by activating brain tissue of the mammal by application of radiation under conditions effective to promote a simultaneous multiphoton excitation of the brain tissue and to emit a fluorescence characteristic. The fluorescence characteristic is then compared to a standard fluorescence emitted by exciting healthy brain tissue of the mammal under the same conditions used to carry out the activating step. Brain tissue where the fluorescence characteristic differs from the standard fluorescence is identified as potentially having a neurodegenerative disease. Another aspect of the present invention is directed to a method of producing an image of brain tissue from a mammal by activating brain tissue of a mammal with radiation applied under conditions effective to promote a simultaneous multiphoton excitation of the brain tissue and to produce fluorescence.Type: ApplicationFiled: October 31, 2001Publication date: January 9, 2003Inventors: Bradley T. Hyman, Richard Christie, Brian Bacskai, Watt W. Webb, Warren R. Zipfel
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Patent number: 6344653Abstract: A laser scanning microscope produces molecular excitation in a target material by simultaneous absorption of three or more photons to thereby provide intrinsic three-dimensional resolution. Fluorophores having single photon absorption in the short (ultraviolet or visible) wavelength range are excited by a beam of strongly focused subpicosecond pulses of laser light of relatively long (red or infrared) wavelength range. The fluorophores absorb at about one third, one fourth or even smaller fraction of the laser wavelength to produce fluorescent images of living cells and other microscopic objects. The fluorescent emission from the fluorophores increases cubicly, quarticly or even higher power law with the excitation intensity so that by focusing the laser light, fluorescence as well as photobleaching are confined to the vicinity of the focal plane.Type: GrantFiled: October 19, 2000Date of Patent: February 5, 2002Inventors: Watt W. Webb, Chris Xu
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Publication number: 20010029316Abstract: The present invention is directed to a method of applying radiation through an optical fiber for detecting disease within a plant or animal or imaging a particular tissue of a plant or animal. In addition, fluorescence can be detected and localized within a subject by such application of radiation through an optical fiber. The radiation is effective to promote simultaneous multiphoton excitation. The optical fibers are used alone to examine internal regions of tissue, in conjunction with an optical biopsy needle to evaluate sub-surface tissue, or with an endoscope to evaluate tissue within body cavities.Type: ApplicationFiled: February 8, 2001Publication date: October 11, 2001Inventor: Watt W. Webb