Abstract: The present invention provides unique methodologies for inducing new bone apposition in-vivo generally or at a localized site and for therapeutically treating a subject afflicted with a pathological disorder of bone which is characterized by a deficiency of calcium ions in the bone matrix. The methodology for treating generalized defective skeletile mineralization comprises administering sufficient quantities of .alpha..sub.2 HS-glycoprotein to the subject such that the concentration of this naturally occurring plasma protein is not substantially less than about 600 micrograms per milliliter of serum in-vivo. Alternatively, the method for treatment of localized defective skeletile mineralization comprises administration of a therapeutic admixture containing about 300 mircograms of .alpha..sub.2 HS-glycoprotein per gram of filler material to the site of skeletal defect.