Abstract: Provided is a proximity ligation assay-based detection method for a high-order structure (HOS) of an RNA virus, including: mixing an RNA virus with a cross-linking agent, conducting cross-linking under ultraviolet (UV) light, and recovering the RNA virus to obtain a cross-linked RNA virus; extracting RNA of the cross-linked RNA virus; conducting fragmentation on the RNA with RNase III to obtain RNA fragments; ligating the RNA fragments and conducting decrosslinking to obtain decrosslinked RNA fragments; constructing a sequencing library for the decrosslinked RNA fragments; and conducting high-throughput sequencing on the sequencing library, and conducting an RNA HOS analysis on a sequencing result. In the present disclosure, high-efficiency short-distance ligation reaction is used to realize the cross-linking of RNA in virus particles in cell culture or collected supernatant, so as to analyze the HOS of RNA virus genome.

Abstract: In a logging identification method for a small fault based on stress anisotropy and crustal stress types, a magnitude and direction of a current crustal stress are determined by means of logging calculation of the crustal stress, and a transformation location of a crustal stress type is determined; a development location of the small fault is determined according to the transformation location of the crustal stress type; and an included angle between the current crustal stress and the fault is determined according to an amplitude of variation of stress anisotropy. The present disclosure provides the logging identification method for a small fault based on stress anisotropy and crustal stress types. According to the method, prediction cost is low, operability is high, and a prediction result has a reference value for guiding exploration and development of a complex oil and gas reservoir and construction of a gas storage.

Abstract: Disclosed are a shallow-buried leakage-proof pipeline and a method for operating the same. The leakage-proof pipeline includes a programmable logic controller (PLC), an outer pipe, an inner pipe, a first water-pressure sensor, a second water-pressure sensor, an electromagnetic valve, and a movable water leakage detection device. The PLC is arranged on the ground. The outer pipe and the inner pipe are both shallowly buried along a horizontal direction, and the inner pipe is coaxially arranged in the outer pipe. The first water-pressure sensor and the electromagnetic valve are both arranged at a water inlet of the inner pipe, and the second water-pressure sensor is arranged at a water outlet of the inner pipe. The movable water leakage detection device is arranged at a bottom of the outer pipe.