Abstract: The invention provides a recombinant Escherichia coli strain for producing succinic acid and a construction method thereof. The by-product encoding genes in the E. coli strain FMME-N-2 are knocked out to obtain the E. coli strain FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA); and the phosphoenolpyruvate carboxykinase pck derived from Actinobacillus succinogenes and the phosphite dehydrogenase ptxD derived from Pseudomonas stutzeri were overexpressed. The constructed plasmid pTrcHisA-pck-ptxD was introduced into the expression host E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA), and the cells were screened in a plate containing ampicillin, to obtain an engineered strain E. coli FMME-N-5 (?focA-pflB-?ldhA-?pta-ackA)-pck-ptxD that can efficiently produce succinic acid. After fermentation by a two-stage fermentation strategy, the production of succinic acid reaches 137 g/L, the yield of succinic acid is up to 1 g/g glucose, and the space time yield is 1.

Abstract: A robotic cleaner includes a housing, a suction conduit with an opening, and a leading roller mounted in front of a brush roll. An inter-roller air passageway may be defined between the leading roller and the brush roll wherein the lower portion of the leading roller is exposed to a flow path to the suction conduit and an upper portion of the leading roller is outside of the flow path. Optionally, a combing unit includes a plurality of combing protrusions extending into the leading roller and having leading edges not aligned with a center of the leading roller. Optionally, a sealing strip is located along a rear side of the opening and along a portion of left and right sides of the opening. The underside may define side edge vacuum passageways extending from the sides of the housing partially between the leading roller and the sealing strip towards the opening.

Abstract: A surface cleaning apparatus including a body defining an agitation chamber, an agitator partially disposed within the agitation chamber and configured to rotate about a pivot axis, and a debrider at least partially disposed within the agitation chamber. The agitator includes an elongated body having a first and a second end, a sidewall extending radially outward from the elongated body extending between the first and the second ends, and a plurality of bristles extending radially outward from the elongated body. The plurality of bristles are arranged in at least one row adjacent to the sidewall. The debrider includes a plurality of teeth configured to contact a portion of the sidewall as the agitator rotates about the pivot axis.

Abstract: A door opening mechanism is operable by an actuator (1140) to open a dirt chamber door (1070) to a dirt collection chamber (1066). The door (1070) is movable about a pivot (1072) between a closed position and an open position. In some examples, the actuator (1140) may be located on the same side of the dirt collection chamber (1066) as the pivot (1072). In some examples, the actuator (1140) may be positioned vertically higher than the pivot (1072) when the dirt collection chamber (1066) is in the emptied position.

Abstract: A robotic cleaner includes a housing, a suction conduit with an opening, and a leading roller mounted in front of a brush roll. An inter-roller air passageway may be defined between the leading roller and the brush roll wherein the lower portion of the leading roller is exposed to a flow path to the suction conduit and an upper portion of the leading roller is outside of the flow path. Optionally, a combing unit includes a plurality of combing protrusions extending into the leading roller and having leading edges not aligned with a center of the leading roller. Optionally, a sealing strip is located along a rear side of the opening and along a portion of left and right sides of the opening. The underside may define side edge vacuum passageways extending from the sides of the housing partially between the leading roller and the sealing strip towards the opening.

Abstract: An example of a vacuum pod may include a handle, a vacuum pod body, a dust cup removably coupled to the vacuum pod body, and a fluid conduit fluidly coupled to the dust cup. The fluid conduit may include a flexible hose configured to transition between an expanded and a retracted position and a coupling configured to be removably coupled to the vacuum pod body. A first end of the flexible hose may be coupled to the vacuum pod body and a second end of the flexible hose may be coupled to the coupling. When the coupling is coupled to the vacuum pod body, the flexible hose may be in the retracted position.

Abstract: A cleaning apparatus may include at least one isolator configured to absorb mechanical vibration generated by contact between an agitator and a combining unit to reduce noise and/or vibration. The isolator may include at least one combing isolator disposed at least partially between the combing unit and the surface cleaning head. Alternatively (or in addition), the isolator may include a panel isolator disposed at least partially between a housing of the cleaning apparatus and a panel.

Abstract: A robotic cleaner includes a housing, a suction conduit with an opening, and a leading roller mounted in front of a brush roll. An inter-roller air passageway may be defined between the leading roller and the brush roll wherein the lower portion of the leading roller is exposed to a flow path to the suction conduit and an upper portion of the leading roller is outside of the flow path. Optionally, a combing unit includes a plurality of combing protrusions extending into the leading roller and having leading edges not aligned with a center of the leading roller. Optionally, a sealing strip is located along a rear side of the opening and along a portion of left and right sides of the opening. The underside may define side edge vacuum passageways extending from the sides of the housing partially between the leading roller and the sealing strip towards the opening.

Abstract: A robotic cleaner includes a housing, a suction conduit with an opening, and a leading roller mounted in front of a brush roll. An inter-roller air passageway may be defined between the leading roller and the brush roll wherein the lower portion of the leading roller is exposed to a flow path to the suction conduit and an upper portion of the leading roller is outside of the flow path. Optionally, a combing unit includes a plurality of combing protrusions extending into the leading roller and having leading edges not aligned with a center of the leading roller. Optionally, a sealing strip is located along a rear side of the opening and along a portion of left and right sides of the opening. The underside may define side edge vacuum passageways extending from the sides of the housing partially between the leading roller and the sealing strip towards the opening.

Abstract: A surface cleaning apparatus having an air flow path from a dirty air inlet to a clean air outlet and including an air treatment member, a suction motor and a filter assembly in the air flow path. The filter assembly includes at least two spaced apart physical filter elements with the upstream face of the second filter facing the downstream face of the first filter element. The filter assembly is configured to prevent air from bypassing the first physical filter media. The filter assembly includes a gap between the first filter element and the second filter element that acts as a header and reduces the flow rate of air incident on the second filter element.

Abstract: A vacuum cleaning apparatus may include a debris container and a filtering system. The debris container may define a debris collection chamber and include a dirty air inlet and a clean air outlet. The filtering system may be at least partially disposed in the debris collection chamber, and may include a fluff screen and a fluff screen cover defining a cavity for receiving at least a portion of the fluff screen. The fluff screen and the fluff screen cover may both include tapered outer surfaces. The tapered outer surfaces may have opposing tapers.

Abstract: A surface cleaning apparatus including a body defining an agitation chamber, an agitator partially disposed within the agitation chamber and configured to rotate about a pivot axis, and a debrider at least partially disposed within the agitation chamber. The agitator includes an elongated body having a first and a second end, a sidewall extending radially outward from the elongated body extending between the first and the second ends, and a plurality of bristles extending radially outward from the elongated body. The plurality of bristles are arranged in at least one row adjacent to the sidewall. The debrider includes a plurality of teeth configured to contact a portion of the sidewall as the agitator rotates about the pivot axis.

Abstract: A surface cleaning apparatus including a body defining an agitation chamber, an agitator partially disposed within the agitation chamber and configured to rotate about a pivot axis, and a debrider at least partially disposed within the agitation chamber. The agitator includes an elongated body having a first and a second end, a sidewall extending radially outward from the elongated body extending between the first and the second ends, and a plurality of bristles extending radially outward from the elongated body. The plurality of bristles are arranged in at least one row adjacent to the sidewall. The debrider includes a plurality of teeth configured to contact a portion of the sidewall as the agitator rotates about the pivot axis.

Abstract: A surface cleaning head includes dual rotating agitators (e.g., a leading roller and a brush roll) and a removable cover for covering a top of the agitators and allowing access to the agitators from a top of the surface cleaning head. The dual agitators may be used to facilitate capturing of debris in the air flow into a suction conduit on the underside of the surface cleaning head. The brush roll may be located in a brush roll chamber and at least partially in an opening to the suction conduit. The leading roller may be positioned adjacent to and in advance of the suction conduit opening such that the leading roller engages debris and moves the debris toward the brush roll and the opening. The leading roller may be removable from the housing and held in place by the removable cover. The cover may be coupled using isolated latching mechanisms.

Abstract: A surface cleaning head includes dual rotating agitators (e.g., a leading roller and a brush roll) and a removable cover for covering a top of the agitators and allowing access to the agitators from a top of the surface cleaning head. The dual agitators may be used to facilitate capturing of debris in the air flow into a suction conduit on the underside of the surface cleaning head. The brush roll may be located in a brush roll chamber and at least partially in an opening to the suction conduit. The leading roller may be positioned adjacent to and in advance of the suction conduit opening such that the leading roller engages debris and moves the debris toward the brush roll and the opening. The leading roller may be removable from the housing and held in place by the removable cover. The cover may be coupled using isolated latching mechanisms.

Abstract: An example of a surface cleaning head may include a main body, a neck pivotally coupled to the main body, a stabilizer, and a linkage pivotally coupled to the main body and the stabilizer. The linkage may be configured to cause the stabilizer to transition between an extended position and a retracted position in response to a pivotal movement of the neck.

Abstract: A cleaning apparatus may include at least one isolator configured to absorb mechanical vibration generated by contact between an agitator and a combining unit to reduce noise and/or vibration. The isolator may include at least one combing isolator disposed at least partially between the combing unit and the surface cleaning head. Alternatively (or in addition), the isolator may include a panel isolator disposed at least partially between a housing of the cleaning apparatus and a panel.

Abstract: An example of a vacuum pod may include a handle, a vacuum pod body, a dust cup removably coupled to the vacuum pod body, and a fluid conduit fluidly coupled to the dust cup. The fluid conduit may include a flexible hose configured to transition between an expanded and a retracted position and a coupling configured to be removably coupled to the vacuum pod body. A first end of the flexible hose may be coupled to the vacuum pod body and a second end of the flexible hose may be coupled to the coupling. When the coupling is coupled to the vacuum pod body, the flexible hose may be in the retracted position.