Abstract: Methods and devices for detecting thrombin generation are disclosed. Generally, the methods include combining a blood sample with a reagent composition so that reaction of the reagent composition and thrombin, if present in the sample, produces a detectable signal; and detecting the detectable signal. Generally, the devices include a fluid-tight material forming at least one passageway; a first chamber in fluid communication with at least one passageway; and at least one reagent disposed on a surface of or contained in either a chamber or a passageway. In some embodiments, the passageway is configured to permit capillary flow of fluid, while in other embodiments, fluid flow is accomplished through a pump functionally linked to at least one passageway. In some embodiments, the device may further include a signal detector positioned to detect a signal generated in a chamber or passageway. In certain embodiments, the device may further include a microprocessor functionally linked to the signal detector.

Abstract: This document provides methods and materials involved in assessing samples (e.g., serum samples) for lipoprotein containing particles. For example, methods and materials involved in using anti-apoprotein antibodies (e.g., fluorescently labeled anti-apoprotein antibodies) to label lipoprotein containing particles that can be detected or measured using flow cytometry are provided.

Abstract: This document relates to methods and materials involved in assessing blood components (e.g., assessing von Willebrand factor activity or platelet activity) in mammals. For example, methods and materials involved in using labeled platelets to assess von Willebrand factor activity in a sample (e.g., plasma) from a mammal (e.g., a human) are provided.

Abstract: This document relates to methods and materials involved in identifying calcification (e.g., coronary calcification or soft tissue calcification) in mammals and assessing thrombotic risk in mammals. For example, methods and materials involved in using microvesicles as a marker to determine whether or not a mammal (e.g., a human) has calcification or an elevated risk of thrombosis are provided. In addition, methods and materials for determining the amount and source of microvesicles are provided.