Abstract: A system and method are disclosed for optically aligning a capillary tube and an excitation laser beam for fluorescence detection applications by utilizing the Raman scatter signals of the capillary tube's contents. For example, Raman scatter by an electrophoretic separation matrix may be used for alignment in a capillary electrophoresis system. Fluorescent material may be present and may also be used for alignment purposes, but is not necessary. The invention employs a parabolic reflector, having apertures through which the capillary tube and the laser beam are guided so that they intersect, preferably at right angles and at the focal point of the reflector. The Raman scatter signals of the material within the capillary tube are collected via a series of filters and this information is used to reposition, if necessary, a focusing lens that directs the excitation beam into the reflector and the capillary tube, so that the Raman scatter signals are maximized.

Abstract: Focusing a generated radiation signal on a detector is effected by a curvilinear surface. The radiation beam signal from the reflected surface is collimated or focused on the detector. Light scattered, Raman scattered, fluorescence, chemiluminescence, phosphorescence radiation signals from particles as a result of a chemical procedure or reaction is enhanced through this focusing technique. The enhanced signal which is detected is subsequently measured through different detection techniques.

Abstract: A method of measuring and compensating stray light in absorbance analysis that use a multiple element array detector wherein one or more of the elements of the diode array are utilized to detect stray radiation in the absence of primary radiation including higher order diffracted radiation. In one aspect, the atmosphere is used to filter all primary radiation below a particular wavelength so that one or more array elements corresponding to detection below such wavelength can be dedicated to the detection of only stray radiation. Detection of higher order diffractions can be prevented by dividing the total spectrum into intervals and detecting these intervals in sequence. In another aspect, a diode array is designed to include additional elements along one side of the array outside the exposure of the primary radiation for the sole purpose of detecting stray radiation.

Abstract: Methods for stray light measurement and compensation in spectrophotometers are disclosed. In one exemplary embodiment, stray light is determined as a convolution of a selected detected radiant power spectrum and a monochromator slit function. Such a stray light measurement may be made with or without the sample in an optical path between a source and detector within the spectrophotometer. When made with the sample in the optical path, the resulting stray light measurement may be used to compensate sample absorbance or transmittance measurements. In accordance with another embodiment of the present invention, sample absorbance or transmittance may be compensated in a method including measuring sample detected radiant power at a wavelength outside an interval of significant detected radiant power within which a sample measurement compensated for stray is desired.

Abstract: Method and apparatus for authenticating documents such as government notes and currency, stock certificates, and the like by marking the document with an encapsulated dyed liquid crystal material and examining the document for unique optical characteristics of the mark. Reading apparatus for the document measures changes in reflectivity of the liquid crystal mark with changes in an electrical field applied thereto. In other embodiments, reflectivity is measured at a plurality of different wavelengths. In one embodiment, the dye absorbs in regions of the electromagnetic spectrum "invisible" to the human eye and the reading apparatus monitors reflectivity changes at these invisible wavelengths.

Abstract: In an optical instrument including a birefringent liquid crystal cell of the type consisting of a pair of transparent plates and a zero-twist, nematic-phase, liquid crystal material with a positive dielectric anisotropy between the plates, the liquid crystal molecules being oriented in and tilting in a plane perpendicular to the cell plates, a beam of light being conducted through the cell, generally normal to the plates thereof, it has been observed that if all of the rays of the beam are not collimated, each ray will suffer a different retardation in passing through the cell and this is unacceptable in most applications. This problem is minimized, according to the present invention, by using a filament light source positioned with the filament axis normal to the plane of the tilting molecule axes or by using two liquid crystal cells between a pair of polarizers, one of the cells being rotated 180.degree.

Abstract: A wavelength calibration solution comprising from about 0.025 to about 0.140 molar neodymium and from about 0.135 to about 0.550 molar samarium, wherein the neodymium is present as a neodymium ionized constituent and the samarium is present as a samarium ionized constituent.A method for checking the wavelength accuracy of spectrometers or spectrophotometers of the type comprising measuring the absorbance (A) or percent transmittance (% T) of a wavelength calibration check solution versus a blank at more than one wavelength. The method is characterized in that the above solution is employed therein as the wavelength calibration check solution.

Abstract: There is disclosed a method and apparatus for implementing a narrow band rejection filter utilizing a liquid crystal cell. A liquid crystal cell is positioned in the path of a light beam between a pair of crossed or parallel polarizers with the optical axis of the cell at a 45.degree. angle to the optical axes of the polarizers. A voltage is applied to the cell to tune the cell to reject a given wavelength and to pass other wavelengths. In order to reject the given wavelength and pass all other wavelengths, the order of the cell is stepped to maintain rejection of a fixed wavelength and the detected signal from the light passing through the cell is averaged.

Abstract: Gain hysteresis exhibited by the photomultiplier detector of a single beam spectrophotometer is reduced by illuminating the detector and applying dynode voltage thereto during the normal "off" or "idle" interval of the spectrophotometer.

Abstract: Gain hysteresis exhibited by the photomultiplier detector of a single beam spectrophotometer is reduced by illuminating the detector and applying dynode voltage thereto during the normal "off" or "idle" interval of the spectrophotometer.

Abstract: Moderately narrow band, tunable, birefringent filters are disclosed using zero-twist liquid crystal cells as variable retarders in a Lyot-type birefringent filter. These filters can achieve analytically useful levels of resolution and stray-light ratios for costs that are competitive with prism and grating monochromators. These birefringent filters can be tuned and/or rendered opaque by the application of a suitable voltage to the cells. Thus, a variety of different optical instruments can be constructed with no moving parts. Power requirements are negligible. A useful filter can be designed to occupy a volume less than one cubic inch. These properties render such a filter ideally suited to microprocessor control.

Abstract: Output current hysteresis exhibited by a multiple-dynode photomultiplier detector is reduced by inactivating one or more of the dynodes by shorting to the detector anode, and operating the detector with the reduced number of active dynodes while retaining the anode as the output current supply terminal.

Abstract: A light scattering photometer including a rotating segmented mirror intercepting light from a laser source and alternately directing the light into a sample zone along an optical axis in first and second opposite directions. A mask annulus coaxial with the optical axis intercepts light scattered from the sample in an incremental angle .DELTA..theta. at a selected angle .theta. with respect to the axis. As the direction of sample illumination alternates, the annulus alternately intercepts forward scatter and back scatter of light from the sample at angles .theta. and 180.degree.-.theta., respectively, and passes the forward and back scatter in alternate succession to a detector. The detector output signal is demodulated to derive near-simultaneous forward and back scatter measurements from the sample. The photometer permits measurement at small values of .theta. approaching 0.degree. and hence permits near-simultaneous scatter measurements at forward and back scatter angles spaced by an angle approaching 180.

Abstract: A method and apparatus for determining the wavelength of a light beam wherein the light beam is directed through a liquid crystal cell functioning as a variable retarder, wherein the voltage applied to the cell is ramped to vary the retardation of the cell between maximum and minimum values, wherein the light from the beam passing through the cell is detected, wherein the intensity of the detected light is monitored, which intensity alternately passes through maximum and minimum values as the retardation of the cell is varied between its maximum and minimum values, and wherein the number of times the intensity alternates between its maximum and minimum values is determined to provide an indication of the wavelength of the light beam.

Abstract: Detector apparatus for a laser light scattering photometer of the type for measuring radiant power scattered from a sample at a selectable angle with respect to the direction of an incident beam of radiant power and for measuring radiant power transmitted through the sample in the direction of the incident beam. The improved detector apparatus receives and detects the scattered power and the transmitted power and provides an output indicative of the radiant power of each independent of the spatial properties of the two beams. The detector apparatus comprises a planar diffuser spaced from and parallel to the large area end-window of a photomultiplier detector, the diffuser diffusing the energy over the surface of the end-window. The diffuser and photomultiplier detector are positioned within a cylindrical container which is enclosed at the end thereof in front of the diffuser, the enclosed end of the container having an aperture therein through which the beams pass to the diffuser.