Patents by Inventor Wilfred A. van der Donk
Wilfred A. van der Donk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240018501Abstract: An isolated nucleic acid that includes an open reading frame encoding a lanthipeptide protease polypeptide for scarless tag removal from a polypeptide is presented. Reagents, expression constructs and methods are also provided for preparing a scarless tag polypeptide product from a tagged polypeptide precursor containing a lanthipeptide protease cleavage site. The reagents are directed to novel lanthipeptide proteases and expression constructs and polypeptide precursors that include highly specific lanthipeptide protease substrate recognition sequence. Methods are provided that enable scarless tag removal from a cognate lanthipeptide, a non-cognate lanthipeptide or a heterologous polypeptide that includes extraneous amino acid sequences, such as leader peptides and tags.Type: ApplicationFiled: November 29, 2022Publication date: January 18, 2024Inventors: Wilfred A. van der Donk, Weixin Tang
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Patent number: 11149270Abstract: Platforms, systems and methods are provided for identifying engineered lanthipeptide display peptides expressed in vivo from biological organisms.Type: GrantFiled: September 28, 2015Date of Patent: October 19, 2021Assignee: The Board of Trustees of the University of IllinoisInventors: Wilfred A. van der Donk, Ayse Okesli, Xiao Yang, Mark Walker, Kenton J. Hetrick
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Patent number: 9993490Abstract: The present invention relates to pharmaceutical compositions comprising a phosphonic acid compound, such as a natural product. Such compounds can include, such as an antibiotic or compound with other activity, derived from an Actinobacteria strain having a gene encoding pepM (phosphoenolpyruvate phosphomutase) or a pepM-dependent biosynthetic pathway. The present invention also relates to methods for treating or preventing or reducing the risk of a bacterial infection by administering a therapeutically effective or prophylactically effective amount of a phosphonic acid antibiotic, or a pharmaceutical composition containing such an antibiotic, to a patient or subject in need thereof. The present invention further relates to methods for isolating, purfying, and identifying such phosphonic acid compounds from Actinobacteria strains.Type: GrantFiled: July 21, 2015Date of Patent: June 12, 2018Assignee: The Board of Trustees of The University of IllinoisInventors: William W. Metcalf, Kou-San Ju, Jiangtao Gao, James R. Doroghazi, Wilfred A. van der Donk
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Publication number: 20170240878Abstract: An isolated nucleic acid that includes an open reading frame encoding a lanthipeptide protease polypeptide for scarless tag removal from a polypeptide is presented. Reagents, expression constructs and methods are also provided for preparing a scarless tag polypeptide product from a tagged polypeptide precursor containing a lanthipeptide protease cleavage site. The reagents are directed to novel lanthipeptide proteases and expression constructs and polypeptide precursors that include highly specific lanthipeptide protease substrate recognition sequence. Methods are provided that enable scarless tag removal from a cognate lanthipeptide, a non-cognate lanthipeptide or a heterologous polypeptide that includes extraneous amino acid sequences, such as leader peptides and tags.Type: ApplicationFiled: May 12, 2015Publication date: August 24, 2017Inventors: Wilfred A. van der Donk, Weixin Tang
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Publication number: 20170204400Abstract: Platforms, systems and methods are provided for identifying engineered lanthipeptide display peptides expressed in vivo from biological organisms.Type: ApplicationFiled: September 28, 2015Publication date: July 20, 2017Applicant: The Board of Trustees of the University of IllinoisInventors: Wilfred A. van der Donk, Ayse Okesli, Xiao Yang, Mark Walker, Kenton J. Hetrick
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Publication number: 20170202863Abstract: The present invention relates to pharmaceutical compositions comprising a phosphonic acid compound, such as a natural product. Such compounds can include, such as an antibiotic or compound with other activity, derived from an Actinobacteria strain having a gene encoding pepM (phosphoenolpyruvate phosphomutase) or a pepM-dependent biosynthetic pathway. The present invention also relates to methods for treating or preventing or reducing the risk of a bacterial infection by administering a therapeutically effective or prophylactically effective amount of a phosphonic acid antibiotic, or a pharmaceutical composition containing such an antibiotic, to a patient or subject in need thereof. The present invention further relates to methods for isolating, purfying, and identifying such phosphonic acid compounds from Actinobacteria strains.Type: ApplicationFiled: July 21, 2015Publication date: July 20, 2017Inventors: William W. Metcalf, Kou-San Ju, Jiangtao Gao, James R. Doroghazi, David P. Labeda, Wilfred A. van der Donk
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Patent number: 9326523Abstract: The disclosure provides lantibiotics geobacillin I and geobacillin II from Geobacillus thermodenitrificans, compositions comprising the lantibiotics, and methods of use of the lantibiotics. Further disclosed are the sequences of lantibiotics geobacillin I and geobacillin I, and alternative structures of geobacillin I and geobacillin II comprising amino acid substitutions. Antimicrobial compositions comprising one or more isolated geobacillins and a pharmaceutically acceptable carrier, and methods of reducing reproduction of bacteria comprising administering to a subject a therapeutically effective amount of the antimicrobial composition are also provided.Type: GrantFiled: February 7, 2013Date of Patent: May 3, 2016Assignee: The Board of Trustees of the University of IllinoisInventors: Wilfred A. van der Donk, Neha Garg, Yuki Goto, Weixin Tang
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Publication number: 20150050256Abstract: The invention provides lantibiotics geobacillin I and geobacillin II from Geobacillus thermodenitrificans, compositions comprising the lantibiotics, and methods of use of the lantibiotics.Type: ApplicationFiled: February 7, 2013Publication date: February 19, 2015Applicant: The Board of Trustees of the University of IllinoisInventors: Wilfred A. van der Donk, Neha Garg, Yuki Goto, Weixin Tang
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Publication number: 20140113874Abstract: Compounds comprising peptides capable of binding C3 protein and inhibiting complement activation are disclosed. These cyclic compounds are modified to improve stability while maintaining substantially equivalent complement activation-inhibitory activity as compared with currently available compounds. The compounds comprise compstatin analogs in which the disulfide bond between C2 and C12 is modified via a thioether bond to form a cystathionine.Type: ApplicationFiled: September 21, 2011Publication date: April 24, 2014Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIAInventors: John D. Lambris, Wilfred A. Van Der Donk
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Patent number: 8372601Abstract: The disclosure of the present application provides polypeptide sequences and nucleotide sequences coding for the polypeptide sequences of proteins used in the production of APPA-containing peptides. In at least one embodiment of the present disclosure, an isolated nucleic acid is disclosed which comprises a nucleotide sequence encoding a polypeptide having a sequence identity of 60 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS: 2-13, and 15-23.Type: GrantFiled: January 21, 2011Date of Patent: February 12, 2013Assignee: University of Illinois at Urbana-ChampaignInventors: William W. Metcalf, Wilfred A. van der Donk, Junkal Zhang, Benjamin T. Circello, Svetlana A. Borisova
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Publication number: 20110217734Abstract: The disclosure of the present application provides polypeptide sequences and nucleotide sequences coding for the polypeptide sequences of proteins used in the production of APPA-containing peptides. In at least one embodiment of the present disclosure, an isolated nucleic acid is disclosed which comprises a nucleotide sequence encoding a polypeptide having a sequence identity of 60 percent or greater to an amino acid sequence selected from the group consisting of SEQ ID NOS: 2-13, and 15-23.Type: ApplicationFiled: January 21, 2011Publication date: September 8, 2011Inventors: William W. Metcalf, Wilfred A. van der Donk, Junkal Zhang, Benajmin T. Circello, Svetlana A. Borisova
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Patent number: 7985837Abstract: The present invention relates to two-component lantibiotics isolated from Bacillus sp. Methods for producing said lantibiotics are provided, wherein dehydration and cyclization of the peptides is carried out by two substrate-specific modifying enzymes. Given the antimicrobial activity of the instant lantibiotics, methods for preventing or treating bacterial infections are also provided.Type: GrantFiled: October 31, 2007Date of Patent: July 26, 2011Assignee: The Board of Trustees of the University of IllinoisInventors: Wilfred A. van der Donk, Lisa E. Cooper, Amanda L. McClerren
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Publication number: 20090325254Abstract: A new xylose reductase encoding gene from Neurspora crassa was heterologously expressed in E. coli as a His-tag fusion protein and subsequently purified in high yield. This xylose reductase was shown to have a high turnover rate and catalytic efficiency, high stability at room temperature, broad pH profile, and a preference of NADPH over NADH. This enzyme is utilized in production of xylitol and other sugar alcohols such as sorbitol and also in the metabolic enhancement of organisms used for fermentation of plant biomass into ethanol.Type: ApplicationFiled: September 1, 2009Publication date: December 31, 2009Applicants: BIOTECHNOLOGY RESEARCH AND DEVELOPMENT CORPORATION, THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOISInventors: Huimin Zhao, Ryan Woodyer, Michael Simurdiak, Wilfred A. van der Donk
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Patent number: 7592163Abstract: A new xylose reductase encoding gene from Neurspora crassa was heterologously expressed in E. coli as a His-tag fusion protein and subsequently purified in high yield. This xylose reductase was shown to have a high turnover rate and catalytic efficiency, high stability at room temperature, broad pH profile, and a preference of NADPH over NADH. This enzyme is utilized in production of xylitol and other sugar alcohols such as sorbitol and also in the metabolic enhancement of organisms used for fermentation of plant biomass into ethanol.Type: GrantFiled: May 19, 2008Date of Patent: September 22, 2009Assignees: The Board of Trustees of the University of Illinois, Biotechnology Research and Development CorporationInventors: Huimin Zhao, Ryan Woodyer, Michael Simurdiak, Wilfred A. van der Donk
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Publication number: 20080241900Abstract: A new xylose reductase encoding gene from Neurspora crassa was heterologously expressed in E. coli as a His-tag fusion protein and subsequently purified in high yield. This xylose reductase was shown to have a high turnover rate and catalytic efficiency, high stability at room temperature, broad pH profile, and a preference of NADPH over NADH. This enzyme is utilized in production of xylitol and other sugar alcohols such as sorbitol and also in the metabolic enhancement of organisms used for fermentation of plant biomass into ethanol.Type: ApplicationFiled: May 19, 2008Publication date: October 2, 2008Applicants: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, BIOTECHNOLOGY RESEARCH AND DEVELOPMENT CORPORATIONInventors: Huimin Zhao, Ryan Woodyer, Michael Simurdiak, Wilfred A. van der Donk
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Publication number: 20080242617Abstract: The present invention relates to two-component lantibiotics isolated from Bacillus sp. Methods for producing said lantibiotics are provided, wherein dehydration and cyclization of the peptides is carried out by two substrate-specific modifying enzymes. Given the antimicrobial activity of the instant lantibiotics, methods for preventing or treating bacterial infections are also provided.Type: ApplicationFiled: October 31, 2007Publication date: October 2, 2008Inventors: Wilfred A. van der Donk, Lisa E. Cooper, Amanda L. McClerren
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Patent number: 7402419Abstract: Phosphite dehydrogenase mutant enzymes were generated that provide relaxed cofactor specificity and increased thermostability over the wild type enzyme. The mutant enzymes are useful for nicotinamide cofactor regeneration.Type: GrantFiled: June 10, 2004Date of Patent: July 22, 2008Assignees: Biotechnology Research and Development Corporation, Board of Trustees of the University of IllinoisInventors: Huimin Zhao, William W. Metcalf, Wilfred A. van der Donk, Tyler Johnannes, Ryan Woodyer
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Patent number: 7381553Abstract: A new xylose reductase encoding gene from Neurspora crassa was heterologously expressed in E. coli as a His-tag fusion protein and subsequently purified in high yield. This xylose reductase was shown to have a high turnover rate and catalytic efficiency, high stability at room temperature, broad pH profile, and a preference of NADPH over NADH. This enzyme is utilized in production of xylitol and other sugar alcohols such as sorbitol and also in the metabolic enhancement of organisms used for fermentation of plant biomass into ethanol.Type: GrantFiled: June 14, 2005Date of Patent: June 3, 2008Assignee: The Board of Trustees of the Universtiy of IllinoisInventors: Huimin Zhao, Ryan Woodyer, Michael Simurdiak, Wilfred A. van der Donk
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Publication number: 20080026999Abstract: The present invention relates to two-component lantibiotics isolated from Bacillus sp. Methods for producing said lantibiotics are provided, wherein dehydration and cyclization of the peptides is carried out by two substrate-specific modifying enzymes. Given the antimicrobial activity of the instant lantibiotics, methods for preventing or treating bacterial infections are also provided.Type: ApplicationFiled: June 26, 2007Publication date: January 31, 2008Inventor: Wilfred A. van der Donk
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Publication number: 20060035353Abstract: A new xylose reductase encoding gene from Neurspora crassa was heterologously expressed in E. coli as a His-tag fusion protein and subsequently purified in high yield. This xylose reductase was shown to have a high turnover rate and catalytic efficiency, high stability at room temperature, broad pH profile, and a preference of NADPH over NADH. This enzyme is utilized in production of xylitol and other sugar alcohols such as sorbitol and also in the metabolic enhancement of organisms used for fermentation of plant biomass into ethanol.Type: ApplicationFiled: June 14, 2005Publication date: February 16, 2006Inventors: Huimin Zhao, Ryan Woodyer, Michael Simurdiak, Wilfred van der Donk