Abstract: The invention relates to clinical applications of quantitative chromatin mapping assays, such as chromatin immunoprecipitation assays and assays using tethered enzymes (e.g., chromatin immunocleavage (ChIC) and cleavage under targets & release using nuclease (CUT&RUN®)). The methods may be used to detect and quantitate the presence of epigenetic modifications and mutations on nucleosomes (histones and/or DNA) from biological samples, monitor changes in the status of modifications and mutations, monitor the effectiveness of epigenetic and mutation therapies, select suitable treatments for a disease, determine the prognosis of a subject, identify biomarkers of a disease, and screen for agents that modify epigenetic or mutation status. The invention further relates to kits for use in the methods of the invention.

Abstract: An exercise handle including a rotatable handle member pivotably connected between two side members by two handle member side pivots. A parallel bar member can be pivotably connected between the two side members spaced away and parallel to the handle member. The parallel bar member can be connected between the two side members by two bar member side pivots. A central support member can be pivotably connected to the parallel bar member by a central pivot, providing the handle member with side to side tilting relative to the central support member. A central joint can be connected to the central support member for connecting to an exercise device.

Abstract: The invention relates generally to methods for preparing recombinant nucleosomes. In particular, the invention relates to methods for ligating a histone peptide onto a fully assembled recombinant nucleosome. The invention further relates to modified core histone proteins, histone peptides to be ligated to the modified core histone proteins, and fully assembled recombinant nucleosomes and libraries of recombinant nucleosomes prepared by the methods of the invention.

Abstract: An assembly comprising a first furniture component and a second furniture component is disclosed. The first furniture component comprises a spring-loaded detent. The spring-loaded detent is biased axially along a central axis toward a locked configuration. The second furniture component comprises a lock arrangement. The first furniture component and the second furniture component are rotatably connected about the central axis through a rotary range of motion. The lock arrangement is configured to move the spring-loaded detent from the locked configuration to an unlocked configuration during the rotary range of motion, release the spring-loaded detent from the unlocked configuration after the rotary range of motion, and prevent rotation of the spring-loaded detent in the locked configuration after the rotary range of motion.

Abstract: Wireless occupancy sensors and methods for using the same are provided. In some embodiments, a occupancy sensor comprises: a housing that includes a window positioned at a top portion of the housing; a battery at a lower portion of the housing; a first magnetometer that detects changes in a magnetic field when a vehicle moves over the first magnetometer; an optical sensor that detects one or more objects in a field of view of the optical sensor through the window; a transmitter for transmitting sensor data to a gateway device, and a processor that controls the first magnetometer, the optical sensor, and the transmitter.

Abstract: The invention relates to assays for nucleosome remodeling activity using functionalized recombinant mononucleosomes. The functionalized recombinant mononucleosomes comprise a histone octamer comprising recombinant histone H2A, H2B, H3 and H4 proteins and a DNA template comprising a nucleosome positioning sequence that effectively positions the histone octamer and a signal site. The invention further relates to methods of using the assay to quantify enzymatic activity of remodeling enzymes and identifying modulators of remodeling enzyme activity.

Abstract: An exercise handle including a rotatable handle member pivotably connected between two side members by two handle member side pivots. A parallel bar member can be pivotably connected between the two side members spaced away and parallel to the handle member. The parallel bar member can be connected between the two side members by two bar member side pivots. A central support member can be pivotably connected to the parallel bar member by a central pivot, providing the handle member with side to side tilting relative to the central support member. A central joint can be connected to the central support member for connecting to an exercise device.

Abstract: The invention relates generally to methods for preparing recombinant nucleosomes. In particular, the invention relates to methods for ligating a histone peptide onto a fully assembled recombinant nucleosome.

Abstract: The invention relates to the use of recombinant/semi-synthetic nucleosomes carrying histone and/or DNA modifications as a reference standard for quantification of covalently modified (on the histone proteins or wrapping DNA), variant, or mutant nucleosomes (collectively “modified nucleosomes” or “nucleosome modifications”) from a biological sample. The invention further relates to methods of using the assay to accurately quantify single or combinatorial nucleosome modifications as biomarkers of disease.

Abstract: The invention relates to clinical applications of quantitative chromatin mapping assays, such as chromatin immunoprecipitation assays and assays using tethered enzymes (e.g., chromatin immunocleavage (ChIC) and cleavage under targets & release using nuclease (CUT&RUN®)). The methods may be used to detect and quantitate the presence of epigenetic modifications and mutations on nucleosomes (histones and/or DNA) from biological samples, monitor changes in the status of modifications and mutations, monitor the effectiveness of epigenetic and mutation therapies, select suitable treatments for a disease, determine the prognosis of a subject, identify biomarkers of a disease, and screen for agents that modify epigenetic or mutation status. The invention further relates to kits for use in the methods of the invention.

Abstract: The invention relates to the use of recombinant/semi-synthetic nucleosomes carrying histone and/or DNA modifications as a reference standard for quantification of covalently modified (on the histone proteins or wrapping DNA), variant, or mutant nucleosomes (collectively “modified nucleosomes” or “nucleosome modifications”) from a biological sample. The invention further relates to methods of using the assay to accurately quantify single or combinatorial nucleosome modifications as biomarkers of disease.

Abstract: The invention relates to assays for nucleosome remodeling activity using functionalized recombinant mononucleosomes. The functionalized recombinant mononucleosomes comprise a histone octamer comprising recombinant histone H2A, H2B, H3 and H4 proteins and a DNA template comprising a nucleosome positioning sequence that effectively positions the histone octamer and a signal site. The invention further relates to methods of using the assay to quantify enzymatic activity of remodeling enzymes and identifying modulators of remodeling enzyme activity.

Abstract: The invention relates to the use of recombinant/semi-synthetic nucleosomes carrying histone and/or DNA modifications as a reference standard for quantification of covalently modified (on the histone proteins or wrapping DNA), variant, or mutant nucleosomes (collectively “modified nucleosomes” or “nucleosome modifications”) from a biological sample. The invention further relates to methods of using the assay to accurately quantify single or combinatorial nucleosome modifications as biomarkers of disease.

Abstract: A mounting assembly is configured for mounting electronics in a ceiling or wall without requiring access to interior portions of the ceiling or wall. The mounting assembly comprises a collar, at least two arms and at least two threaded screws. The collar fits around and attaches to the electronics housing. The at least two arms are each independently compressed to engage the ceiling and are coupled to the collar to support the collar in the ceiling.

Abstract: A mounting assembly is configured for mounting electronics in a ceiling or wall without requiring access to interior portions of the ceiling or wall. The mounting assembly comprises a collar, at least two arms and at least two threaded screws. The collar fits around and attaches to the electronics housing. The at least two arms are each independently compressed to engage the ceiling and are coupled to the collar to support the collar in the ceiling.