Abstract: Colorless single component TMB reagents for the detection of HRP and their processes of manufacture including the encapsulation of substrate within a cyclodextrin cavity are described.

Abstract: Contaminants in particulate materials may be separated and quantitated by contacting the particulate material with an immobilized biomolecule which specifically binds to the contaminant, separating the contaminant-immobilized biomolecule complex from the particulate material, and counting the bound contaminant. The method may be conveniently carried out when the biomolecule is immobilized on a magnetic particle. An apparatus for carrying out the method contains a receptacle for receiving a reaction vessel and magnets positioned such that magnetic particles contained in the reaction vessel will be drawn to and adhere to the sides of the reaction vessel when placed in the receptacle.

Abstract: Medium molecular weight alcohols (for example aliphatic alcohols, such as trihydroxypentane and trihydroxyhexane, and aromatic alcohols, such as benzylalcohol) have been found to provide unexpected stability to dye and stain solutions. In addition, the use of these alcohols does not involve the negatives found in other dye stabilizers, for example extreme flammability and toxicity. The novel alcohols have been found to provide benefit to many dye systems.

Abstract: Alkaline phosphatase isoenzymes are separated electrophoretically in agarose gels, or cellulose acetate membranes, equilibrated in a solution containing a low ionic strength buffer and a non-ionic detergent, such as Triton X-100. The electrophoretically separated isoenzymes are developed colorimetrically using 5-bromo-4-chloro-3-indolyl phosphate with a transphosphorylating buffer. Zones of enzyme activity appear as sharply-defined brilliant blue bands. The method is capable of resolving liver, bone, placental, intestinal and bile isoenzymes.