Abstract: A method and corresponding optical device to correct spherical aberration in an optical path caused by an electrically tunable lens (ETL) within the optical path. The method includes placing within the optical path and in working relationship with the ETL an aspherical correction lens dimensioned and configured to reduce spherical aberration in a light beam exiting the ETL.

Abstract: A method and corresponding optical device to correct spherical aberration in an optical path caused by an electrically tunable lens (ETL) within the optical path. The method includes placing within the optical path and in working relationship with the ETL an aspherical correction lens dimensioned and configured to reduce spherical aberration in a light beam exiting the ETL.

Abstract: A Z-axis focusing mechanism having a scanner and a prism whose front and rear faces are not normal to the direction of travel of a light beam prior to the light beam impinging upon the front face of the scanner. The scanner and the prism are oriented with respect to one another such that when a light beam is scanned onto the front face of the prism, a scanned beam having varying Z-axis focus points at distinct lateral locations exits the rear face of the scanner.

Abstract: Disclosed is an apparatus for division and rearrangement of light from a source object. The apparatus splits the light from the source object, or image of the source object, and recombines it in a parallel, fashion to increase the efficiency of multiphoton microscopy in general and harmonic or fluorescence emission microscopy in particular. The apparatus includes a beam splitter configured to split a light beam into at least two independent light paths to yield a first light path and a second light path; a first beam focuser configured to direct and focus the first light path onto a focal plane; and a second beam focuser configured to direct and focus the second light path onto the same or different focal plane to which the first light path is focused; and wherein the first and second light paths may be superimposed upon one another at a common focal plane or directed independently to different positions.

Abstract: Disclosed is an apparatus for division and rearrangement of light from a source object. The apparatus splits the light from the source object, or image of the source object, and recombines it in a parallel, fashion to increase the efficiency of multiphoton microscopy in general and harmonic or fluorescence emission microscopy in particular. The apparatus includes a beam splitter configured to split a light beam into at least two independent light paths to yield a first light path and a second light path; a first beam focuser configured to direct and focus the first light path onto a focal plane; and a second beam focuser configured to direct and focus the second light path onto the same or different focal plane to which the first light path is focused; and wherein the first and second light paths may be superimposed upon one another at a common focal plane or directed independently to different positions.

Abstract: Described is a scanning microscope that includes at least two scanners disposed in series within an excitation beam, wherein one of the scanners is a two-axis galvanometer-controlled scanner, and the other of the scanners is a single-axis resonant scanner. The device may also include a spatial detection system disposed within the excitation beam at a point downstream of the at least two scanners, wherein the spatial detection system is configured to detect a sum of deflections generated by the at least two scanners, or to detect angular differences in the excitation beam when two or more illumination sources are used.

Abstract: Disclosed are microscopes, preferably confocal microscopes, that include aperture plates having one or more arrays of apertures defined therein. The aperture plate is disposed in a position within an incoming light beam such that the incoming light beam is spatially filtered as it passes through the array of apertures. In certain embodiments of the invention, the aperture plate is fixed and immovable, in others, the aperture plate is translatable within a plane of confocality.

Abstract: Disclosed are microscopes, preferably confocal microscopes, that include aperture plates having one or more arrays of apertures defined therein. The aperture plate is disposed in a position within an incoming light beam such that the incoming light beam is spatially filtered as it passes through the array of apertures. In certain embodiments of the invention, the aperture plate is fixed and immovable, in others, the aperture plate is translatable within a plane of confocality.