Abstract: The present disclosure describes technologies that permit sensitive detection of nucleic acids of interest (i.e., nucleic acids whose nucleotide sequence is or includes a target sequence). Among other things the disclosure provides a system comprising: a plurality of nucleic acid molecules having different nucleotide sequences; a set of ligation oligonucleotides, comprising: a first ligation oligonucleotide whose nucleotide sequence includes a templating element and a first target hybridization element; and a second ligation oligonucleotide whose nucleotide sequence includes a second target hybridization element and optionally a second templating element; wherein the target hybridization elements bind to different portions of a common target site, to form a gapped nucleic acid strand susceptible to ligation with a ligase to generate a ligated strand that is amenable to lateral flow assessment.

Abstract: Certain aspects of the present invention provide methods for assembling nucleic acid molecules using iterative activation of one or more vector-encoded traits to progressively assemble a longer nucleic acid insert. Aspects of the invention also provide kits, compositions, devices, and systems for assembling synthetic nucleic acids using iterative activation of one or more vector-encoded traits.

Abstract: Certain aspects of the present invention provide methods for assembling nucleic acid molecules using iterative activation of one or more vector-encoded traits to progressively assemble a longer nucleic acid insert. Aspects of the invention also provide kits, compositions, devices, and systems for assembling synthetic nucleic acids using iterative activation of one or more vector-encoded traits.

Abstract: Certain aspects of the present invention provide methods for assembling nucleic acid molecules using iterative activation of one or more vector-encoded traits to progressively assemble a longer nucleic acid insert. Aspects of the invention also provide kits, compositions, devices, and systems for assembling synthetic nucleic acids using iterative activation of one or more vector-encoded traits.

Abstract: Certain aspects of the present invention provide methods for assembling nucleic acid molecules using iterative activation of one or more vector-encoded traits to progressively assemble a longer nucleic acid insert. Aspects of the invention also provide kits, compositions, devices, and systems for assembling synthetic nucleic acids using iterative activation of one or more vector-encoded traits.

Abstract: An inner member is telescopically mounted within an outer tubular member and is normally locked against telescopic sliding movement relative to the outer member by a coupling mounted for back and forth manual manipulation along the outside of the two telescoping members at the location where the two members are coupled together. Manual manipulation of the coupling in one direction unlocks the inner member to permit sliding movement thereof relative to the outer member and the coupling, so long as the coupling is urged manually in the one direction. When the coupling is released, it automatically locks the inner member against further sliding movement.

Abstract: A telescoping, pole-type lure retriever has a snare portion that wraps around a fishing line and is guided thereon for removing a fishing lure that is snagged on an underwater obstruction or weeds. An elongated pole has a number of telescoping sections that may be extended to a convenient length to disengage the lure. Each telescoping section may be locked in position by turning a locking nut that compresses a locking ring in a cammed fashion to grip a smaller diameter pole section. The snake portion has a knurled shank seated in locking engagement with a sleeve member and with the sleeve fitted in the end of one pole section.

Abstract: A waste gate for an internal combustion engine having an exhaust gas driven supercharger supplying air under pressure to the engine intake manifold which is sensitive to the intake manifold pressure and reduces the quantity of air supplied to the intake manifold by the supercharger when the pressure in the intake manifold attains a predetermined value for efficient use of fuel supplied to the engine and increasing its power output.