Abstract: A brush overlay Pap smear method is provided for providing a single cytological sample representing specimens for two or more sites. An exocervical sample (20) is transferred from a scraper (40) to an end portion (16) of a surface (14) of a microscope slide (10). Subsequently, an endocervical sample is combined with the exocervical sample (20) and distributed across the surface (14) of slide (10) to provide a substantially uniform distribution of the combined samples into a substantially uniform film (22) on the surface (14) of slide (10). In an alternate embodiment, the application of the exocervical sample is preceded by the application of exfoliated cells obtained from the posterior fornix of the vagina, the exocervical sample being applied to the exfoliated cell sample (30) to form a combined sample (32).