Patents by Inventor William M. Bonner

William M. Bonner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods and kits for measuring and quantifying DNA double-stranded breaks using gamma-H2AX and H2AX
    Patent number: 10809268
    Abstract: Disclosed are methods of measuring the amount of exposure of a host to a DNA double-stranded break (DSB)-causing agent by determining the ratio of the quantity of ?-H2AX to the quantity of total H2AX in a biological sample from the host as compared to the ratio of the quantity of ?-H2AX to the quantity of total H2AX in a positive control biological sample that has been exposed to a known amount of a DSB-causing agent. Related kits and methods of quantifying DSBs in a test biological sample are also disclosed.
    Type: Grant
    Filed: February 1, 2016
    Date of Patent: October 20, 2020
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Service
    Inventors: Christophe E. Redon, William M. Bonner, Yiping Zhang, Jiuping Ji
  • METHODS AND KITS FOR MEASURING AND QUANTIFYING DNA DOUBLE-STRANDED BREAKS USING GAMMA-H2AX AND H2AX
    Publication number: 20180003720
    Abstract: Disclosed are methods of measuring the amount of exposure of a host to a DNA double-stranded break (DSB)-causing agent by determining the ratio of the quantity of ?-H2AX to the quantity of total H2AX in a biological sample from the host as compared to the ratio of the quantity of ?-H2AX to the quantity of total H2AX in a positive control biological sample that has been exposed to a known amount of a DSB-causing agent. Related kits and methods of quantifying DSBs in a test biological sample are also disclosed.
    Type: Application
    Filed: February 1, 2016
    Publication date: January 4, 2018
    Applicant: The United States of America,as represented by the Secretary,Department of Health and Human Service
    Inventors: Christophe E. Redon, William M. Bonner, Yiping Zhang, Jiuping Ji
  • Kit for determining DNA double-stranded breaks with anti-?-H2A antibodies
    Patent number: 6884873
    Abstract: The present invention provides an isolated or purified antibody or antigenically-reactive fragment thereof that specifically binds to a C-terminal phosphorylated serine in an H2A histone protein and a product comprising the same. The present invention further provides fusion proteins comprising the isolated or purified antibody or antigenically-reactive fragment thereof. Also provided by the present invention are a method and a kit for determining double-stranded breaks in DNA. The method comprises contacting a sample comprising H2A histone proteins with the isolated or purified antibody or antigenically-reactive fragment thereof and detecting binding of the antibody or fragment thereof to an H2A histone protein in the sample. The detection of the binding of the antibody or fragment thereof to the H2A histone protein indicates the presence of a DNA double-stranded break in DNA.
    Type: Grant
    Filed: November 7, 2001
    Date of Patent: April 26, 2005
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: William M. Bonner, Emmy Rogakou
  • Kit for determining DNA double-stranded breaks with anti-gamma-H2A antibodies
    Publication number: 20020132989
    Abstract: The present invention provides an isolated or purified antibody or antigenically-reactive fragment thereof that specifically binds to a C-terminal phosphorylated serine in an H2A histone protein and a product comprising the same. The present invention further provides fusion proteins comprising the isolated or purified antibody or antigenically-reactive fragment thereof. Also provided by the present invention are a method and a kit for determining double-stranded breaks in DNA. The method comprises contacting a sample comprising H2A histone proteins with the isolated or purified antibody or antigenically-reactive fragment thereof and detecting binding of the antibody or fragment thereof to an H2A histone protein in the sample. The detection of the binding of the antibody or fragment thereof to the H2A histone protein indicates the presence of a DNA double-stranded break in DNA.
    Type: Application
    Filed: November 7, 2001
    Publication date: September 19, 2002
    Inventors: William M. Bonner, Emmy Rogakou
  • Anti-&ggr;-H2A antibody, fusion proteins thereof and method and kit for determining DNA double-stranded breaks
    Patent number: 6362317
    Abstract: The present invention provides an isolated or purified antibody or antigenically-reactive fragment thereof that specifically binds to a C-terminal phosphorylated serine in an H2A histone protein and a product comprising the same. The present invention further provides fusion proteins comprising the isolated or purified antibody or antigenically-reactive fragment thereof. Also provided by the present invention are a method and a kit for determining double-stranded breaks in DNA. The method comprises contacting a sample comprising H2A histone proteins with the isolated or purified antibody or antigenically-reactive fragment thereof and detecting binding of the antibody or fragment thereof to an H2A histone protein in the sample. The detection of the binding of the antibody or fragment thereof to the H2A histone protein indicates the presence of a DNA double-stranded break in DNA.
    Type: Grant
    Filed: July 12, 1999
    Date of Patent: March 26, 2002
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: William M. Bonner, Emmy Rogakou