Abstract: Methods of inducing an immune response comprising at least two administrations of an adjuvanted immunogenic composition, wherein the second administration contains reduced amounts of antigen and adjuvant.

Abstract: A needle bending assembly configured to at least temporarily contain a needle can include a first housing section, a second housing section, and a coupling element coupled between the first housing section and the second housing section. The first housing section defines a first cavity configured to at least temporarily contain a first section of the needle. The second housing section defines a second cavity configured to at least temporarily contain a second section of the needle forming a distal tip. The coupling element is configured such that when the needle is contained within the housing sections, movement of the second housing section relative to the first housing section results in a bending of the needle at a location corresponding to the coupling element.

Abstract: A tape rule comprises a blade, a reel, a spring, a lock, and a housing. The blade comprises a substrate and a coating. The coating is configured to provide color, environmental protection, and/or abrasion resistance. In some embodiments, (1) the coating is formed from non-polymer materials; (2) the blade comprises reinforcements metallurgically bonded to the blade at or near a free end; (3) measurement indicia comprise laser marked portions of the substrate that create color contrasts with other portions of the substrate; (4) the measurement indicia comprise portions of the substrate visible through gaps in the coating; (5) the measurement indicia comprise voids in the substrate filled with a coating material having a contrasting color; and/or (6) the substrate has a surface texture configured to change an appearance of the coating relative to an appearance of the coating on an as-rolled surface finish of the metal substrate.

Abstract: A vehicle cleaning system that can be manufactured to integrate with an existing motor vehicle or as part of a new vehicle manufacture, providing a convenient automated method of cleaning a large motor vehicle such as a trailer or RV. The vehicle cleaning system can be implemented by delivering water from either an independent valve running to an onboard cleaning system; a water line connection; or from an onboard holding tank which may contain water or other cleaning agents, to a valve system operating a series of extrusion nozzles. The cleaning system may have one or more manual or electromechanical pumps and a manual or electromechanical valve system to actuate at least one cleaning cycle, or to activate the operation of the vehicle cleaning system in specific zones along the body of a vehicle. The vehicle cleaning system may operate by means of a roof-mounted actuated piping and nozzle system or an extrusion nozzle spray system having recessed extendable nozzles, or an integrated nozzle system.

Abstract: A fluid pump apparatus for an artificial lift system has a barrel, a standing valve positioned at a lower end of the barrel so as to be movable between an open position and a closed position, a plunger reciprocatingly mounted in the barrel, a traveling valve positioned in an interior of the plunger so as to control fluid flow through the plunger, and a pilot slidably positioned in the plunger. The plunger has a first aperture at an upper portion thereof and a second aperture extending through a wall of the plunger so as to open to a channel extending through the channel. The traveling valve is slidably movable within an interior of the plunger. The plunger has a seat that is cooperative with a surface of the traveling valve. The pilot is cooperative at the surface of the traveling valve so as to move the traveling valve.

Abstract: A tape rule comprises a blade, a reel, a spring, a lock, and a housing. The blade comprises a substrate and a coating. The coating is configured to provide color, environmental protection, and/or abrasion resistance. In some embodiments, (1) the coating is formed from non-polymer materials; (2) the blade comprises reinforcements metallurgically bonded to the blade at or near a free end; (3) measurement indicia comprise laser marked portions of the substrate that create color contrasts with other portions of the substrate; (4) the measurement indicia comprise portions of the substrate visible through gaps in the coating; (5) the measurement indicia comprise voids in the substrate filled with a coating material having a contrasting color; and/or (6) the substrate has a surface texture configured to change an appearance of the coating relative to an appearance of the coating on an as-rolled surface finish of the metal substrate.

Abstract: A fluid pump apparatus for an artificial lift system has a barrel, a standing valve positioned at a lower end of the barrel, a plunger reciprocatingly mounted within the barrel, and a traveling valve positioned in an interior of the plunger so as to control fluid flow through the plunger. The barrel has an opening at a top thereof and an opening at a lower end thereof. The standing valve is movable between an open position and a closed position. The plunger has a first aperture at an upper portion of the plunger and a second aperture extending through a wall of the plunger so as to open to a channel extending longitudinally through the plunger. The traveling valve has a head portion of the body extending downwardly from the head portion. The body is slidably movable within an interior of the plunger. The body has a fluid-passing channel therein that opens to an exterior of the body.

Abstract: A fluid pump apparatus for an artificial lift system has a barrel, a standing valve positioned at a lower end of the barrel so as to be movable between an open position and a closed position, a plunger reciprocatingly mounted in the barrel, a traveling valve positioned in an interior of the plunger so as to control fluid flow through the plunger, and a pilot slidably positioned in the plunger. The plunger has a first aperture at an upper portion thereof and a second aperture extending through a wall of the plunger so as to open to a channel extending through the channel. The traveling valve is slidably movable within an interior of the plunger. The plunger has a seat that is cooperative with a surface of the traveling valve. The pilot is cooperative at the surface of the traveling valve so as to move the traveling valve.

Abstract: A hydraulic fluid distribution system includes a mechanical switch including a first valve, a first actuation pin extending axially from the first valve, and a second actuation pin extending axially from the first valve. The first valve includes an inlet port, a first outlet port, and a second outlet port. The first valve has a first position with the inlet port in fluid communication with the first outlet port and a second position with the inlet port in fluid communication with the second outlet port. The first actuation pin is configured to move in a first axial direction to transition the first valve from the second position to the first position, and the second actuation pin is configured to move in a second axial direction to transition the first valve from the first position to the second position.

Abstract: A piston includes a piston housing and a decompression valve disposed in the piston housing. The decompression valve includes a valve housing seated in the piston housing and a valve member moveably received by the valve housing. The valve member has a radially outer surface including an annular shoulder. In addition, the piston includes an end cap secured to the first end of the piston housing. A radially inner surface of the end cap includes an annular valve seat. The decompression valve has a closed position with the annular shoulder of the valve member engaging the valve seat of the end cap and an open position with the annular shoulder of the valve member axially spaced from the valve seat of the end cap. The piston also includes a biasing member configured to bias the decompression valve to the closed position.

Abstract: A fluid pump apparatus for an artificial lift system has a barrel, a standing valve positioned at a lower end of the barrel, a plunger reciprocatingly mounted within the barrel, and a traveling valve positioned in an interior of the plunger so as to control fluid flow through the plunger. The barrel has an opening at a top thereof and an opening at a lower end thereof. The standing valve is movable between an open position and a closed position. The plunger has a first aperture at an upper portion of the plunger and a second aperture extending through a wall of the plunger so as to open to a channel extending longitudinally through the plunger. The traveling valve has a head portion of the body extending downwardly from the head portion. The body is slidably movable within an interior of the plunger. The body has a fluid-passing channel therein that opens to an exterior of the body.

Abstract: Carbon monoxide (CO) is a member of the gasotransmitter family that includes NO and H2S and is implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. We now report a new type of turn-on fluorescent probe for selective CO detection by exploiting palladium-mediated carbonylation reactivity. The compounds of the invention are capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.

Abstract: A piston includes a piston housing and a decompression valve disposed in the piston housing. The decompression valve includes a valve housing seated in the piston housing and a valve member moveably received by the valve housing. The valve member has a radially outer surface including an annular shoulder. In addition, the piston includes an end cap secured to the first end of the piston housing. A radially inner surface of the end cap includes an annular valve seat. The decompression valve has a closed position with the annular shoulder of the valve member engaging the valve seat of the end cap and an open position with the annular shoulder of the valve member axially spaced from the valve seat of the end cap. The piston also includes a biasing member configured to bias the decompression valve to the closed position.

Abstract: A hydraulic fluid distribution system includes a mechanical switch including a first valve, a first actuation pin extending axially from the first valve, and a second actuation pin extending axially from the first valve. The first valve includes an inlet port, a first outlet port, and a second outlet port. The first valve has a first position with the inlet port in fluid communication with the first outlet port and a second position with the inlet port in fluid communication with the second outlet port. The first actuation pin is configured to move in a first axial direction to transition the first valve from the second position to the first position, and the second actuation pin is configured to move in a second axial direction to transition the first valve from the first position to the second position.

Abstract: A deliquification pump for deliquifying a well comprises a fluid end pump adapted to pump a fluid from a wellbore. In addition, the deliquification pump comprises a hydraulic pump adapted to drive the fluid end pump. The hydraulic pump includes a first internal pump chamber and a first pump assembly disposed in the first chamber. The first pump assembly includes a piston having a first end, a second end, and a throughbore extending between the first end and the second end. In addition, the first pump assembly includes a first wobble plate including a planar end face axially adjacent the second end of the piston and a slot extending axially through the first wobble plate. The first wobble plate is adapted to rotate about the central axis relative to the housing to axially reciprocate the piston and cyclically place the throughbore of the piston in fluid communication with the slot.

Abstract: Carbon monoxide (CO) is a member of the gasotransmitter family that includes NO and H2S and is implicated in a variety of pathological and physiological conditions. Whereas exogenous therapeutic additions of CO to tissues and whole animals have been well studied, the real-time spatial and temporal tracking of CO at the cellular level remains an open challenge. We now report a new type of turn-on fluorescent probe for selective CO detection by exploiting palladium-mediated carbonylation reactivity. The compounds of the invention are capable of detecting CO both in aqueous buffer and in live cells with high selectivity over a range of biologically relevant reactive small molecules, providing a potentially powerful approach for interrogating its chemistry in biological systems.

Abstract: A deliquification pump for deliquifying a well comprises a fluid end pump adapted to pump a fluid from a wellbore. In addition, the deliquification pump comprises a hydraulic pump adapted to drive the fluid end pump. The hydraulic pump includes a first internal pump chamber and a first pump assembly disposed in the first chamber. The first pump assembly includes a piston having a first end, a second end, and a throughbore extending between the first end and the second end. In addition, the first pump assembly includes a first wobble plate including a planar end face axially adjacent the second end of the piston and a slot extending axially through the first wobble plate. The first wobble plate is adapted to rotate about the central axis relative to the housing to axially reciprocate the piston and cyclically place the throughbore of the piston in fluid communication with the slot.

Abstract: A deliquification pump for deliquifying a well comprises a fluid end pump adapted to pump a fluid from a wellbore. In addition, the deliquification pump comprises a hydraulic pump adapted to drive the fluid end pump. The hydraulic pump includes a first internal pump chamber and a first pump assembly disposed in the first chamber. The first pump assembly includes a piston having a first end, a second end, and a throughbore extending between the first end and the second end. In addition, the first pump assembly includes a first wobble plate including a planar end face axially adjacent the second end of the piston and a slot extending axially through the first wobble plate. The first wobble plate is adapted to rotate about the central axis relative to the housing to axially reciprocate the piston and cyclically place the throughbore of the piston in fluid communication with the slot.

Abstract: A deliquification pump for deliquifying a well comprises a fluid end pump adapted to pump a fluid from a wellbore. In addition, the deliquification pump comprises a hydraulic pump adapted to drive the fluid end pump. The hydraulic pump includes a first internal pump chamber and a first pump assembly disposed in the first chamber. The first pump assembly includes a piston having a first end, a second end, and a throughbore extending between the first end and the second end. In addition, the first pump assembly includes a first wobble plate including a planar end face axially adjacent the second end of the piston and a slot extending axially through the first wobble plate. The first wobble plate is adapted to rotate about the central axis relative to the housing to axially reciprocate the piston and cyclically place the throughbore of the piston in fluid communication with the slot.

Abstract: A deliquification pump for deliquifying a well comprises a fluid end pump adapted to pump a fluid from a wellbore. In addition, the deliquification pump comprises a hydraulic pump adapted to drive the fluid end pump. The hydraulic pump includes a first internal pump chamber and a first pump assembly disposed in the first chamber. The first pump assembly includes a piston having a first end, a second end, and a throughbore extending between the first end and the second end. In addition, the first pump assembly includes a first wobble plate including a planar end face axially adjacent the second end of the piston and a slot extending axially through the first wobble plate. The first wobble plate is adapted to rotate about the central axis relative to the housing to axially reciprocate the piston and cyclically place the throughbore of the piston in fluid communication with the slot.