Abstract: A genetically modified mammalian cell and genetically modified mammalian cell line comprise a recombination sequence inserted in a target locus on a chromosome of the mammalian cell genome, wherein the recombination sequence comprises Bxb1attB sequence from Mycobacterium smegmatis. A transgenic mammalian cell and transgenic mammalian cell line comprise a heterologous nucleic acid stably integrated in a target locus on a chromosome of the mammalian genome, wherein the heterologous nucleic comprises a heterologous gene configured for expression by the transgenic mammalian cell.

Abstract: Apparatus for making electrical pins with frusto-conical end portions, comprises a wire feed station for the supply of a length of metal wire to a wire severing station at which a wire severing wheel severs the wire into pin blanks, in cooperation with a fixed bushing. The wheel pushes the blanks along a track to a pin blank rolling station at which the end portion of the pin blanks are rolled to frusto-conical shape between a pin rolling fixture and a pin rolling wheel having mounted for rotation relative thereto, a pin blank entraining ring having pin blank receiving grooves.