Abstract: A fluorescent dye comprising a xanthene-derived fluorophore having the formula (I) wherein R1, R2, R3, R4, R5 and R6 are independently selected from H, alkyl, alkoxy, alcohol, ether, alkenyl, alkenoxy, aryl, alkaryl, aralkyl and amido, except that R1, R4 and/or R5 is not H when bonded to Y, Y1 or Y2, respectively; X is either O? or S?; and at least one of Y, Y1, Y2, Y3, Y4 and Y5 is a group for covalently bonding the dye, optionally through. the use of a coupling agent, to a target molecule, and is otherwise H. The dye may be covalently attached to a target molecule to form a complex, and the dye and/or complex finds use in cell analysis techniques, particularly pH measurement and analysis of kinetics of migration.

Abstract: The present invention describes a rapid and efficient in vivo library-versus-library screening strategy for identifying optimally interacting pairs of heterodimerizing polypeptides. It allows for the screening of a protein library against a second protein library and therefore finds numerous applications in the study of protein-protein interactions. Two leucine zipper libraries, semi-randomized at the positions adjacent to the hydrophobic core, were genetically fused to either one of two designed fragments of the enzyme murine dihydrofolate reductase (mDHFR), and cotransformed into E. coli. Interaction between the library polypeptides was required for reconstitution of the enzymatic activity of mDHFR, allowing bacterial growth. Using more weakly associating mDHFR fragments, we increased the stringency of selection. We applied these selection processes to a library-versus-library sample of 2.

Abstract: The instant invention describes a method for detecting protein-protein interactions in living organisms and/or cells, said method comprising: (a) synthesizing probe protein fragments from a protein which enables fluorescent or luminescent detection by dissecting the gene coding for the fluorescent or luminescent protein into a least two fragments; (2) constructing fusion proteins consisting of the probe protein fragments linked to protein domains that are to be tested for interactions; (3) coexpressing the fusion proteins; and (d) detecting reconstitution of the fluorescence or luminescence signal.

Abstract: A mica-based electrically-conductive filler and reinforcing material, and methods of producing it. The material comprises milled mica particles and an electrically-conductive media such as carbon black or silver or copper adhered to the mica by a binder system. The material contributes both electrical conductivity and improved physical properties such as increased stiffness, increased tensile strength, and increased scratch resistance to materials for antistatic and electromagnetic shielding such as thermoplastic and thermosetting polymers, in a cost-effective manner relative to alternative prior art electrically-conductive and reinforcing materials.

Abstract: A magnetic field is generated by at least one coil in magnetic communication with at least a portion of a vehicle responsive to a first time-varying signal operatively coupled to the at least one coil in series with a sense resistor. A second signal is generated responsive to a voltage across the sense resistor and is response to a magnetic condition of the at least one coil, which is response to the magnetic communication of the at least one coil with the portion of the vehicle.

Abstract: A magnetic field generated by at least one coil operatively associated with a first portion of a vehicle responsive to a first signal from a signal source causes an eddy current in at least one conductive element operatively associated with or at least a part of a second portion of the vehicle. In one aspect, a circuit operatively coupled to the at least one coil generates a second signal responsive to a self-impedance of the at least one coil, wherein the second signal is at least partially in-phase with the first signal. In another aspect, a magnetic sensor is responsive to an eddy current in the at least one conductive element. In yet another aspect, the at least one conductive element comprises a metal sheet, film or coating of paramagnetic or diamagnetic material that is relatively highly conductive.

Abstract: A sleeved composite pipe or piling structure formed of an elongated polyethylene pipe or tube of high-density polyethylene (HDPE) or another polyethylene material installed over a substantially rigid and incompressible hollow metal pipe or solid wood core having an outer diameter that is the same or slightly larger than a normal inside diameter of the polyethylene pipe or tube when measured in a relaxed state at ambient temperature. The polyethylene pipe or tube is, for example, a HDPE 3408 material formed of virgin PE 3408 resin as specified in ASTM D3350 with UV protection, and the pipe is produced to ASTM A-3408. The metal pipe core can be ferrous or nonferrous pipe.

Abstract: The instant invention describes a method for detecting protein-protein interactions in living organisms and/or cells, said method comprising: (a) synthesizing probe protein fragments from a protein which enables fluorescent or luminescent detection by dissecting the gene coding for the fluorescent or luminescent protein into a least two fragments; (2) constructing fusion proteins consisting of the probe protein fragments linked to protein domains that are to be tested for interactions; (3) coexpressing the fusion proteins; and (d) detecting reconstitution of the fluorescence or luminescence signal.

Abstract: A vibrating inertial rate sensor has operational elements that define axes that are rotationally offset or “skewed” from a node or anti-node reference axis. The skew may be relative to separate node or anti-node reference axes, or take the form of an element that is “split” about the same node axis. Both the drive signal and the sense signal may be resolved from a common set of sensing elements. The drive elements may also operate on a skewed axis angle to rotationally offset the vibration pattern to affect active torquing of the gyroscope. Skewed drive elements may be combined with skewed or split elements on the same device. The skewed sensing scheme may be applied to vibratory systems having one or more node axes. The skewed drive scheme may be applied to vibratory systems having two or more node axes to affect active torquing.

Abstract: A vibrating inertial rate sensor has sense elements that operate on axes that are rotationally skewed from a node reference axis, enabling both a rate sense and a drive sense determination. Alternatively, the skew may be applied to rotationally offset the drive elements from antinode reference axes to affect active torquing of the gyroscope. The skewed sensing scheme may be applied to vibratory systems having one or more node axes. The skewed drive scheme may be applied to vibratory systems having two or more node axes to affect active torquing.

Abstract: A system (105) determines a power level for transmitting to a neighboring node in a wireless network. The system (105) receives a message indicating a three-dimensional position and orientation of the neighboring node and a type of directional antenna of the neighboring node that transmitted the message. The system (105) determines the power level for transmitting to the neighboring node based on the three-dimensional position and orientation of the neighboring node and the type of the directional antenna.

Abstract: A drive circuit apparatus for use in generating a drive signal for energizing an actuator about a natural resonant frequency is disclosed. The circuit has a counter that generates a count sequence derived from a drive sense signal. Additionally, a demodulator is further coupled to the counter and generates a voltage level signal from the drive sense signal based on the count sequence. A digital to analog (D/A) converter is coupled to both the counter and demodulator. The D/A converter generates the drive signal in a substantially constant phase relationship with respect to the drive sense signal as derived from the voltage level signal and based on the count sequence. In addition, a method of generating a drive signal for energizing an actuator about a natural resonant frequency is provided.

Abstract: The present invention is directed to Protein-fragment Complementation Assays (PCAs) and assay compositions based on fluorescent proteins. The invention provides methods for fragmenting fluorescent proteins and generating mutant fragments with desired spectral characteristics for PCA. The invention encompasses assays and compositions based on fluorescent proteins from the species Aequorea, Anemonia and Anthozoa. In particular, the invention is directed to fragments of mutant fluorescent proteins having improved spectral properties over the wild-type proteins. The invention encompasses fragments of mutant versions of A. Victoria green fluorescent protein (GFP), in particular yellow fluorescent proteins (EYFP and super-EYFP), ‘Venus’, cyan, ‘citrine’, blue, cyan-green, and photoactivatable variants of GFP.

Abstract: The present invention describes a method for detecting biomolecular interactions said method comprising: (a) selecting an appropriate reporter molecule selected from the group consisting of a protein, a fluorescent protein, a luminescent protein and a phosphorescent protein; (b) effecting fragmentation of said reporter molecule such that said fragmentation results in reversible loss of reporter function; (c) fusing or attaching fragments of said reporter molecule separately to other molecules; followed by (d) reassociation of said reporter fragments through interactions of the molecules that are fused to said fragments; and (e) detecting said biomolecular interactions by reconstitution of activity of the reporter molecule.

Abstract: The present invention relates to aniline derivatives of the general formula I or pharmaceutically acceptable salts thereof, [Formula (I)], and their use.

Abstract: A sleeved composite pipe or piling structure formed of an elongated polyethylene pipe or tube of high-density polyethylene (HDPE) or another polyethylene material installed over a substantially rigid and incompressible hollow metal pipe or solid wood core having an outer diameter that is the same or slightly larger than a normal inside diameter of the polyethylene pipe or tube when measured in a relaxed state at ambient temperature. The polyethylene pipe or tube is, for example, a HDPE 3408 material formed of virgin PE 3408 resin as specified in ASTM D3350 with UV protection, and the pipe is produced to ASTM A-3408. The metal pipe core can be ferrous or nonferrous pipe.

Abstract: The present invention relates to aniline derivatives of the general formula I or pharmaceutically acceptable salts thereof and their use.

Abstract: The present invention relates to aniline derivatives of the general formula I or pharmaceutically acceptable salts thereof and their use.

Abstract: The present invention relates to morpholine and thiomorpholine derivatives of the general formula I or pharmaceutically acceptable salts thereof and their use.

Abstract: The present invention relates to aniline derivatives of the general formula I or salts thereof and their use.