Abstract: This invention relates to the use of a tunicate or an extract obtained from a tunicate for the production of one or more biofuel selected from an alcohol and biodiesel. The invention also relates to a method for producing a biofuel from a tunicate wherein the biofuel is selected from an alcohol and biodiesel and wherein said method comprises the steps of: (a)(i) subjecting said tunicate or one or more polysaccharides extracted from said tunicate to enzymatic or acid hydrolysis to form a hydrolysate containing one or more monosaccharides and (ii) fermenting said one or more monosaccharides to form an alcohol; or (b)(i) extracting lipids/fatty acids from said tunicate and (ii) converting said lipids/fatty acids into biodiesel by transesterification or alcoholysis or (iii) subjecting said tunicate to transesterification or alcoholysis thereby converting lipids/fatty acids present in said tunicate into biodiesel.