Patents by Inventor Wim Soetaert
Wim Soetaert has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240035055Abstract: This disclosure is in the technical field of cell cultivation or fermentation for the production of oligosaccharides. The present application discloses a process for purification of an oligosaccharide solution produced by microbial fermentation.Type: ApplicationFiled: August 10, 2021Publication date: February 1, 2024Inventors: Joeri Beauprez, Maarten Delforche, Gaspard Lequeux, Wim Soetaert, Thomas Verbrugghen
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Publication number: 20240011062Abstract: This disclosure is in the technical field of cell cultivation or fermentation for the production of oligosaccharides. This disclosure discloses a method to produce a purified mixture of different oligosaccharides produced by a cell cultivation or microbial fermentation.Type: ApplicationFiled: August 10, 2021Publication date: January 11, 2024Inventors: Joeri Beauprez, Maarten Delforche, Gaspard Lequeux, Wim Soetaert, Thomas Verbrugghen
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Publication number: 20230406875Abstract: This specification relates to a process for preparing a purified human milk oligosaccharide (“HMO”) from an HMO-containing solution (e.g., a fermentation broth) by a process comprising mixed bed ion exchange, and a product of such a process.Type: ApplicationFiled: September 28, 2021Publication date: December 21, 2023Inventors: Jyrki Kuusisto, Tero Tuomas Mentunen, Wim Soetaert
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Publication number: 20230175032Abstract: The present invention relates to the field of production of novel biosurfactants. More specifically, the present invention relates to the efficient generation of short chained on-glycosides with less than 10%, preferably less than 1%, ?-1 glycosides using a fungal strain such as the yeast Starmerella bombicola having a dysfunctional CYP52M1 cytochrome P450 monooxygenase and a dysfunctional FAO1 fatty alcohol oxidase to produce high amounts of so-called unsaturated (symmetrical) ?,?-bola glycosides free from contaminating ?,?-1 bola glycosides, and subjecting said unsaturated (symmetrical) ?,?-bola glycosides to conditions inducing the breaking of the present double bond(s) such as for example through ozonolysis performed in water. More specifically, the present invention discloses the generation of (acetylated) C9:0 ?-sophoroside aldehydes, C9:0 ?-glucoside aldehydes, C9:0 ?-glucolipids, C9:0 ?-sophorolipids, C9:0 ?-sophoroside alcohols and C9:0 ?-glucoside alcohols and their further derivatives.Type: ApplicationFiled: May 12, 2021Publication date: June 8, 2023Inventors: Wim SOETAERT, Sophie ROELANTS, Christian STEVENS, Elisabeth DELBEKE, Goedele LUYTEN, Melike PALA, Jelle REMMERY
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Publication number: 20220033869Abstract: The present invention relates to the field of microbial production of novel biosurfactants. More specifically, the present invention discloses the usage of a fungal strain such as the yeast Starmerellabombicola having a dysfunctional CYP52M1 cytochrome P450 monooxygenase and a dysfunctional FAO1 fatty alcohol oxidase for producing high amounts of so-called “symmetrical bolaform sophorosides” where both sophorose moieties are attached through a terminal glycosidic linkage to the hydrophobic linker. In addition, the present invention further discloses that the latter yeast can also be used to produce alkyl sophorosides and symmetrical bolaform glucosides.Type: ApplicationFiled: November 21, 2019Publication date: February 3, 2022Applicants: UNIVERSITEIT GENT, BIO BASE EUROPE PILOT PLANTInventors: Sophie Roelants, Lisa Van Renterghem, Wim Soetaert, Jelle Remmery
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Patent number: 9650658Abstract: This disclosure relates to the field of the microbial production of specific types of glycolipids that are potentially useful as replacements for petroleum-based detergents and emulsifiers. This disclosure, more specifically, discloses the usage of yeasts having a dysfunctional acetyltransferase for producing high amounts of bolaamphiphilic glycolipids when using conventional and cheap substrates. Furthermore, the disclosure relates to yeasts having a dysfunctional acetyltransferase optionally combined with a dysfunctional lactonase and/or second glucosyltransferase. The latter microorganisms are capable of producing high amounts of bolaamphiphilic sophorolipids.Type: GrantFiled: August 7, 2014Date of Patent: May 16, 2017Assignee: Universiteit GentInventors: Wim Soetaert, Inge Van Bogaert, Sophie Roelants
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Patent number: 9394559Abstract: Described is an enzyme derived from Candida bombicola that is capable of lactonizing or polymerizing carbohydrate-containing compounds, lipids, fatty acids, hydroxylated fatty acids, alcohols, dicarboxylic acids or mixtures thereof. Hence, host cells comprising the enzyme can be used, via the formation of intra- or inter-molecular ester-bounds, to produce, for example, lactonized sophorolipids or polymers of acidic sophorolipids. Also described is that host cells having lost their capability to produce a functional enzyme disclosed herein can be used to produce 100% acidic sophorolipids.Type: GrantFiled: June 18, 2015Date of Patent: July 19, 2016Assignee: Universiteit GentInventors: Inge Van Bogaert, Katarzyna Ciesielska, Bart Devreese, Wim Soetaert, Sophie Roelants
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Patent number: 9388443Abstract: The present disclosure relates to an enzyme derived from Candida bombicola that is capable of lactonizing or polymerizing carbohydrate-containing compounds, lipids, fatty acids, hydroxylated fatty acids, alcohols, dicarboxylic acids or mixtures thereof. Hence, host cells comprising the latter enzyme can be used, via the formation of intra- or inter-molecular ester-bounds, to produce, for example, lactonized sophorolipids or polymers of acidic sophorolipids. On the other hand, host cells having lost their capability to produce a functional enzyme disclosed herein can be used to produce 100% acidic sophorolipids.Type: GrantFiled: December 14, 2012Date of Patent: July 12, 2016Assignee: Universiteit GentInventors: Inge Van Bogaert, Katarzyna Ciesielska, Bart Devreese, Wim Soetaert, Sophie Roelants
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Publication number: 20160168612Abstract: This disclosure relates to the field of the microbial production of specific types of glycolipids that are potentially useful as replacements for petroleum-based detergents and emulsifiers. This disclosure, more specifically, discloses the usage of yeasts having a dysfunctional acetyltransferase for producing high amounts of bolaamphiphilic glycolipids when using conventional and cheap substrates. Furthermore, the disclosure relates to yeasts having a dysfunctional acetyltransferase optionally combined with a dysfunctional lactonase and/or second glucosyltransferase. The latter microorganisms are capable of producing high amounts of bolaamphiphilic sophorolipids.Type: ApplicationFiled: August 7, 2014Publication date: June 16, 2016Inventors: Wim Soetaert, Inge Van Bogaert, Sophie Roelants
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Publication number: 20150284761Abstract: Described is an enzyme derived from Candida bombicola that is capable of lactonizing or polymerizing carbohydrate-containing compounds, lipids, fatty acids, hydroxylated fatty acids, alcohols, dicarboxylic acids or mixtures thereof. Hence, host cells comprising the enzyme can be used, via the formation of intra- or inter-molecular ester-bounds, to produce, for example, lactonized sophorolipids or polymers of acidic sophorolipids. Also described is that host cells having lost their capability to produce a functional enzyme disclosed herein can be used to produce 100% acidic sophorolipids.Type: ApplicationFiled: June 18, 2015Publication date: October 8, 2015Inventors: Inge Van Bogaert, Katarzyna Ciesielska, Bart Devreese, Wim Soetaert, Sophie Roelants
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Patent number: 9102922Abstract: The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogs and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism Caldanaerobacter subterraneus, and mutated types thereof, which are highly thermostable and have a broad acceptor and donor specificity.Type: GrantFiled: December 9, 2011Date of Patent: August 11, 2015Assignee: Universiteit GentInventors: Jef Van Der Borght, Tom Desmet, Wim Soetaert, Chao Chen
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Patent number: 9005923Abstract: The present invention relates to yeast species which are normally capable of producing sophorolipids but which are modified in such way that they are incapable producing the latter compounds. These sophorolipid-negative strains surprisingly display equal growth characteristics and biomass formation as their wild type counterparts and are hence useful for the production of compounds such as recombinant proteins, glycolipids, polyhydroxyalkanoates and carotenoides. In addition, the present invention discloses two glucosyltransferase genes with key-functions in sophorolipid production.Type: GrantFiled: June 10, 2011Date of Patent: April 14, 2015Assignee: Universiteit GentInventors: Wim Soetaert, Sofie De Maeseneire, Karen Saerens, Sophie Roelants, Inge Van Bogaert
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Patent number: 8889379Abstract: An in vitro method to produce a glycoside is described which includes the steps of contacting the cellodextrin phosphorylase from Clostridium stercorarium with alpha-glucose-1-phosphate or alpha-galactose-1-phosphate and an acceptor, and glycosylating the acceptor. The acceptor may be an alkyl beta-glucoside, an aryl beta-glucoside, a glucolipid, an alkyl beta-sophoroside, an aryl beta-sophoroside or a sophorolipid. Alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids are produced when alpha-glucose-1-phosphate is used as donor. Corresponding lactosides are produced when alpha-galactose-1-phosphate is used as donor.Type: GrantFiled: May 19, 2011Date of Patent: November 18, 2014Assignee: Universiteit GentInventors: Giang Hai Tran, Tom Desmet, Wim Soetaert
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Publication number: 20140335567Abstract: The present disclosure relates to an enzyme derived from Candida bombicola that is capable of lactonizing or polymerizing carbohydrate-containing compounds, lipids, fatty acids, hydroxylated fatty acids, alcohols, dicarboxylic acids or mixtures thereof. Hence, host cells comprising the latter enzyme can be used, via the formation of intra- or inter-molecular ester-bounds, to produce, for example, lactonized sophorolipids or polymers of acidic sophorolipids. On the other hand, host cells having lost their capability to produce a functional enzyme disclosed herein can be used to produce 100% acidic sophorolipids.Type: ApplicationFiled: December 14, 2012Publication date: November 13, 2014Inventors: Inge Van Bogaert, Katarzyna Ciesielska, Bart Devreese, Wim Soetaert, Sophie Roelants
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Publication number: 20130302857Abstract: The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogues and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism Caldanaerobacter subterraneus, and mutated types thereof, which are highly thermostable and have a broad acceptor and donor specificity.Type: ApplicationFiled: December 9, 2011Publication date: November 14, 2013Applicant: Universiteit GentInventors: Jef Van Der Borght, Tom Desmet, Wim Soetaert, Chao Chen
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Publication number: 20130089892Abstract: The present invention relates to yeast species which are normally capable of producing sophorolipids but which are modified in such way that they are incapable producing the latter compounds. These sophorolipid-negative strains surprisingly display equal growth characteristics and biomass formation as their wild type counterparts and are hence useful for the production of compounds such as recombinant proteins, glycolipids, polyhydroxyalkanoates and carotenoides. In addition, the present invention discloses two glucosyltransferase genes with key-functions in sophorolipid production.Type: ApplicationFiled: June 10, 2011Publication date: April 11, 2013Applicant: Universiteit GentInventors: Wim Soetaert, Sofie De Maeseneire, Karen Saerens, Sofie Roelants, Inge Van Bogaert
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Publication number: 20130059340Abstract: The present invention relates to the biocatalytic production of glycosides. In particular, the invention discloses a method to produce alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids via the usage of a cellodextrin phosphorylase (CDP) derived from Clostridium stercorarium (CsCDP). The method of the present invention is feasible for use in industrial glycosylation processes and has advantageous properties compared to classical as chemical glycosylation reactions. Moreover, said method can also be employed to produce corresponding lactosides such a lactolipids when a-galactose-1-phosphate instead of a-glucose-1-phosphate is used as a donor.Type: ApplicationFiled: May 19, 2011Publication date: March 7, 2013Applicant: UNIVERSITEIT GENTInventors: Giang Hai Tran, Tom Desmet, Wim Soetaert
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Publication number: 20130029384Abstract: The present invention relates to a sucrose phosphorylase from Bifidobacterium adolescentis which is useful as a biocatalyst in carbohydrate conversions at high temperatures. Indeed, the biocatalysts of the present invention are enzymatically active for a time period of at least 16 h and up to 1 to 2 week(s) at a temperature of at least 60° C. The biocatalysts of the present invention are: a) immobilized on an enzyme carrier, or b) are part of a cross-linked enzyme aggregate (CLEA), and/or c) are mutated, and/or d) are enzymatically active in the continuous presence of their substrate.Type: ApplicationFiled: April 1, 2011Publication date: January 31, 2013Applicant: UNIVERSITEIT GENTInventors: An Cerdobbel, Tom Desmet, Wim Soetaert
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Publication number: 20120311741Abstract: The invention relates to a transporter protein involved in the transport of sophorolipids. More specifically, it relates to a Candida bombicola sophorolipid transporter protein, and the use of this transporter to modulate the secretion and/or production of glycolipids, preferably sophorolipids in organisms, preferably in fungi.Type: ApplicationFiled: December 9, 2010Publication date: December 6, 2012Inventors: Wim Soetaert, Inge Van Bogaert
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Patent number: 8278073Abstract: The present invention relates to novel lactose phosphorylase enzymes and the uses thereof. More specifically, the invention relates to lactose phosphorylase enzymes created by mutation of a cellobiose phosphorylase from Cellulomonas uda. By introducing mutations in this enzyme, the activity can be switched from cellobiose phosphorylase into lactose phosphorylase.Type: GrantFiled: December 19, 2008Date of Patent: October 2, 2012Assignee: Universiteit GentInventors: Tom Desmet, Manu De Groeve, Wim Soetaert