Patents by Inventor Wolfgang Mundt

Wolfgang Mundt has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of isolation and purification of trypsin from pronase protease and use thereof
    Patent number: 8389261
    Abstract: The present invention provides methods of isolation and purification of Streptomyces griseus trypsin (SGT) from PRONASE protease mixture in a single affinity chromatography step and uses of the purified SGT.
    Type: Grant
    Filed: December 28, 2009
    Date of Patent: March 5, 2013
    Assignee: Baxter Healthcare S.A.
    Inventors: Artur Mitterer, Christa Tauer, Manfred Reiter, Wolfgang Mundt
  • Recombinant cell clones having increased stability and methods of making and using the same
    Patent number: 8329465
    Abstract: Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
    Type: Grant
    Filed: January 6, 2011
    Date of Patent: December 11, 2012
    Assignee: Baxter Innovations GmbH
    Inventors: Manfred Reiter, Wolfgang Mundt, Friedrich Dorner
  • METHOD OF PRODUCING RECOMBINANT HIGH MOLECULAR WEIGHT vWF IN CELL CULTURE
    Publication number: 20120035110
    Abstract: Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.
    Type: Application
    Filed: July 8, 2011
    Publication date: February 9, 2012
    Applicants: Baxter Healthcare S.A., Baxter International Inc.
    Inventors: LEOPOLD GRILLBERGER, MANFRED REITER, WOLFGANG MUNDT
  • METHOD OF ISOLATION AND PURIFICATION OF TRYPSIN FROM PRONASE PROTEASE AND USE THEREOF
    Publication number: 20120003691
    Abstract: The present invention provides methods of isolation and purification of Streptomyces griseus trypsin (SGT) from PRONASE protease mixture in a single affinity chromatography step and uses of the purified SGT.
    Type: Application
    Filed: December 28, 2009
    Publication date: January 5, 2012
    Applicant: Baxter Healthcare S.A.
    Inventors: Artur Mitterer, Christa Tauer, Manfred Reiter, Wolfgang Mundt
  • Recombinant cell clones having increased stability and methods of making and using the same
    Patent number: 8084252
    Abstract: Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
    Type: Grant
    Filed: August 2, 2010
    Date of Patent: December 27, 2011
    Assignee: Baxter Innovations GmbH
    Inventors: Manfred Reiter, Wolfgang Mundt, Friedrich Dorner
  • Recombinant cell clones having increased stability and methods of making and using the same
    Patent number: 8084251
    Abstract: Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
    Type: Grant
    Filed: May 12, 2010
    Date of Patent: December 27, 2011
    Assignee: Baxter Innovations GmbH
    Inventors: Manfred Reiter, Wolfgang Mundt, Friedrich Dorner
  • Recombinant cell clones having increased stability and methods of making and using the same
    Patent number: 8080414
    Abstract: Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
    Type: Grant
    Filed: June 19, 2009
    Date of Patent: December 20, 2011
    Assignee: Baxter Innovations GmbH
    Inventors: Manfred Reiter, Wolfgang Mundt, Friedrich Dorner
  • MEDIUM FOR THE PROTEIN-FREE AND SERUM-FREE CULTIVATION OF CELLS
    Publication number: 20110287482
    Abstract: A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.
    Type: Application
    Filed: August 4, 2011
    Publication date: November 24, 2011
    Applicant: Baxter Innovations GmbH
    Inventors: Manfred Reiter, Wolfgang Mundt, Friedrich Dorner, Leopold Grillberger, Artur Mitterer
  • Method for producing mature VWF from VWF pro-peptide
    Patent number: 8058411
    Abstract: The present invention relates to a method for producing a mature von Willebrand Factor (VWF) from von Willebrand Factor pro-peptide comprising the steps: immobilizing VWF pro-peptide on an ion exchange resin, incubating the immobilized VWF pro-peptide with furin to obtain immobilized mature VWF, and isolating mature VWF from the ion exchange resin by elution.
    Type: Grant
    Filed: May 16, 2008
    Date of Patent: November 15, 2011
    Assignees: Baxter International Inc., Baxter Healthcare S.A.
    Inventors: Wolfgang Mundt, Artur Mitterer, Meinhard Hasslacher, Christa Mayer
  • Medium for the protein-free and serum-free cultivation of cells
    Patent number: 8021881
    Abstract: A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.
    Type: Grant
    Filed: August 20, 2007
    Date of Patent: September 20, 2011
    Assignee: Baxter Innovations GmbH
    Inventors: Manfred Reiter, Wolfgang Mundt, Friedrich Dorner, Leopold Grillberger, Artur Mitterer
  • ANIMAL PROTEIN FREE MEDIA FOR CULTIVATION OF CELLS
    Publication number: 20110217772
    Abstract: The present invention relates to animal protein free cell culture media comprising a combination of non-animal derived peptides derived from soy hydrolysate and yeast hydrolysate. The invention also provides an animal protein free culture process, wherein cells are cultivated, propagated and passaged without animal-derived components. This process is useful for cultivating cells, such as recombinant cells or cells infected with a virus, and for production biological products by cell culture processes under conditions devoid of animal protein components.
    Type: Application
    Filed: May 6, 2011
    Publication date: September 8, 2011
    Applicants: Baxter International Inc., Baxter Healthcare S.A.
    Inventors: Manfred Reiter, Wolfgang Mundt, Leopold Grillberger, Barbara Kraus
  • OLIGOPEPTIDE-FREE CELL CULTURE MEDIA
    Publication number: 20110151512
    Abstract: The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.
    Type: Application
    Filed: February 25, 2011
    Publication date: June 23, 2011
    Applicants: Baxter International Inc., Baxter Healthcare S.A.
    Inventors: Leopold Grillberger, Manfred Reiter, Wolfgang Mundt, Artur Mitterer
  • Animal protein free media for cultivation of cells
    Patent number: 7955833
    Abstract: The present invention relates to animal protein free cell culture media comprising a combination of non-animal derived peptides derived from soy hydrolysate and yeast hydrolysate. The invention also provides an animal protein free culture process, wherein cells are cultivated, propagated and passaged without animal-derived components. This process is useful for cultivating cells, such as recombinant cells or cells infected with a virus, and for production biological products by cell culture processes under conditions devoid of animal protein components.
    Type: Grant
    Filed: July 8, 2003
    Date of Patent: June 7, 2011
    Assignees: Baxter International Inc., Baxter Healthcare, S.A.
    Inventors: Manfred Reiter, Wolfgang Mundt, Leopold Grillberger, Barbara Kraus
  • RECOMBINANT CELL CLONES HAVING INCREASED STABILITY AND METHODS OF MAKING AND USING THE SAME
    Publication number: 20110104758
    Abstract: Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
    Type: Application
    Filed: January 6, 2011
    Publication date: May 5, 2011
    Applicant: Baxter Aktiengesellschaft
    Inventors: Manfred Reiter, Wolfgang Mundt, Friedrich Dorner
  • Animal Protein-Free Media For Cultivation of Cells
    Publication number: 20110081680
    Abstract: The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.
    Type: Application
    Filed: December 10, 2010
    Publication date: April 7, 2011
    Applicants: Baxter International Inc., Baxter Healthcare S.A.
    Inventors: Leopold Grillberger, Manfred Reiter, Wolfgang Mundt, Friedrich Dorner
  • ANIMAL PROTEIN-FREE MEDIA FOR CULTIVATION OF CELLS
    Publication number: 20110081722
    Abstract: The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.
    Type: Application
    Filed: May 17, 2010
    Publication date: April 7, 2011
    Applicants: Baxter International Inc., Baxter Healthcare S.A.
    Inventors: Leopold Grillberger, Manfred Reiter, Wolfgang Mundt, Friedrich Dorner
  • Recombinant Cell Clones Having Increased Stability and Methods of Making and Using the Same
    Publication number: 20100317055
    Abstract: Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
    Type: Application
    Filed: August 2, 2010
    Publication date: December 16, 2010
    Applicant: Baxter Aktiengesellschaft
    Inventors: Manfred Reiter, Wolfgang Mundt, Friedrich Dorner
  • Two-Step Temperature Profile for the Propagation of Viruses
    Publication number: 20100239606
    Abstract: The present invention provides a method for the production of a virus. The method includes providing a host cell that has been infected by the virus and cultivating the infected host cell at two different temperatures. The virus produced by the cultivation steps is subsequently collected. By using the dual temperature cultivation process, high titer and improved purity can be obtained.
    Type: Application
    Filed: April 30, 2008
    Publication date: September 23, 2010
    Applicants: BAXTER INTERNATIONAL INC., BAXTER HEALTHCARE S.A.
    Inventors: Manfred Reiter, Leopold Grillberger, Wolfgang Mundt
  • Formulation of Sugar Solutions for Continuous Ultracentrifugation for Virus Purification
    Publication number: 20100239609
    Abstract: The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.
    Type: Application
    Filed: April 30, 2008
    Publication date: September 23, 2010
    Applicants: Baxter International Inc., Baxter Healthcare S.A.
    Inventors: Manfred Reiter, Leopold Grillberger, Wolfgang Mundt, Artur Mitterer, Horst Schafhauser
  • RECOMBINANT CELL CLONES HAVING INCREASED STABILITY AND METHODS OF MAKING AND USING THE SAME
    Publication number: 20100221828
    Abstract: Disclosed are a stable recombinant cell clones which are stable in serum- and protein-free medium for at least 40 generations, a biomass obtained by multiplying the stable cell clone under serum- and protein-free culturing conditions, and a method of preparing recombinant proteins by means of the biomass. Furthermore, the invention relates to a method of recovering stable recombinant cell clones.
    Type: Application
    Filed: May 12, 2010
    Publication date: September 2, 2010
    Applicant: Baxter Aktiengesellschaft
    Inventors: Manfred Reiter, Wolfgang Mundt, Friedrich Dorner