Abstract: A method for in-vitro tissue cultivation. The method may include providing a seeded-scaffold to a scaffold holder suspended in a medium contained in a bioreactor chamber. The method may further include rotating, via a rotation mechanism which the bioreactor chamber is coupled to, the bioreactor chamber about two orthogonal axes based on a predetermined motion cycle as a stimulation for tissue growth. The method may further include applying, via a stimulator coupled to the bioreactor chamber, at least one other stimulation for tissue growth to the seeded-scaffold. An apparatus for in-vitro tissue cultivation.

Abstract: The invention relates to a bioreactor comprising a chamber for containing cells or tissue cultures within a culture medium. The bioreactor also comprises a detector capable of detecting a change in one or more metabolites associated with growth of the cell or tissue cultures within the chamber and a chamber drive capable of rotating the chamber at a first speed about a first axis and a second speed about a second axis, the second axis being disposed at an angle relative to the first axis. In use, the magnitude of the first speed and the second speed are independently variable of each other.

Abstract: The invention relates to a continuous flow dual axis bioreactor (10) for growing three-dimensional cell or tissue cultures. The bioreactor (10) includes a chamber (12) adapted to contain a three-dimensional matrix for growing three-dimensional cell or tissue cultures. A rotatable plate (14) supports the chamber (12) and is rotatable about a vertical axis (16). The rotatable plate (14) is supported on an rotary L-shaped bracket (20) that rotates about a horizontal axis (22). Two multi-flow fluid connectors (32,34) are provided to prevent any pipes connecting the chamber to a feed source or a product tank from being entangled and to allow continuous flow to and from the bioreactor (10).