Patents by Inventor Wyatt N. Vreeland
Wyatt N. Vreeland has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9198645Abstract: Methods for the formation of liposomes that encapsulate reagents in a continuous 2-phase flow microfluidic network with precision control of size, for example, from 100 nm to 300 nm, by manipulation of liquid flow rates are described. By creating a solvent-aqueous interfacial region in a microfluidic format that is homogenous and controllable on the length scale of a liposome, fine control of liposome size and polydispersity can be achieved.Type: GrantFiled: July 21, 2004Date of Patent: December 1, 2015Assignee: The United States of America, as represented by the Secretary of Commerce of The National Institute of Standards and TechnologyInventors: Andreas Jahn, Wyatt N. Vreeland, Laurie E. Locascio, Michael Gaitan
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Publication number: 20130168885Abstract: A device and method for the formation of vesicles is disclosed herein. The device comprises a fluid introduction zone and a vesicle formation zone. The fluid introduction zone comprises a first inlet and a second inlet configured and disposed to provide parallel flow of an outer flow stream, flowing from the first inlet, sheathing an inner flow stream, flowing from the second inlet. The vesicle formation zone is configured and disposed to receive the parallel flow of the outer flow stream sheathing the inner flow stream and configured for a controlled and substantially uniform dispersion of an organic material, flowing in the inner flow stream, at a plane perpendicular to the vesicle formation zone.Type: ApplicationFiled: January 3, 2013Publication date: July 4, 2013Inventors: DONNA M. OMIATEK, RENEE R. HOOD, FRANCISCO JAVIER ATENCIA-FERNANDEZ, DON L. DEVOE, WYATT N. VREELAND
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Publication number: 20120141997Abstract: The present invention provides compositions and methods for performing free-solution conjugate analysis of nucleic acid molecules. For example, the present invention provides multiplexed single-base extension assays for genotyping. In particular, the present invention provides a series of disperse polyamide “drag tags” for use in achieving high-resolution separation of nucleic acid reaction products.Type: ApplicationFiled: February 14, 2012Publication date: June 7, 2012Applicant: NORTHWESTERN UNIVERSITYInventors: Robert J. Meagher, Wyatt N. Vreeland, Annelise E. Barron
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Patent number: 8114599Abstract: The present invention provides compositions and methods for performing free-solution conjugate analysis of nucleic acid molecules. For example, the present invention provides multiplexed single-base extension assays for genotyping. In particular, the present invention provides a series of disperse polyamide “drag tags” for use in achieving high-resolution separation of nucleic acid reaction products.Type: GrantFiled: December 19, 2007Date of Patent: February 14, 2012Assignee: Northwestern UniversityInventors: Robert J. Meagher, Wyatt N Vreeland, Annelise E. Barron
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Patent number: 7718046Abstract: A method and device are provided for affinity gradient focusing for directing at least one analyte in a solution containing a pseudostationary phase and located in a channel such as a capillary or a microchannel. The method includes establishing a steady-state spatial gradient in a retention factor of the pseudostationary phase for the at least one analyte. The analyte is caused to be moved within the channel whereby the concentration of the at least one analyte changes at one or more positions along the gradient. The pseudostationary phase is charged and the analyte is either neutral or charged or alternatively, the pseudostationary phase is neutral and the analyte is charged. The device may include a fluid channel, a pseudostationary phase having a retention factor gradient, an electrical current source and a pump system for establishing the bulk flow in the solution in the channel.Type: GrantFiled: June 10, 2004Date of Patent: May 18, 2010Assignee: The United States of America, as represented by the Secretary of Commerce, the National Institute of Standards & TechnologyInventors: David J. Ross, Peter B. Howell, Wyatt N. Vreeland
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Patent number: 7572357Abstract: A method and device are provided for concentrating and separating materials in fluids within a fluidic device having a fluid conduit such as a channel or capillary. The concentration is achieved by balancing the electrophoretic velocity of a material against the bulk flow of fluid in the presence of a temperature gradient. An additive is added to the fluid which interacts with the material and which modifies the normal electrophoretic mobility of the material. Using an appropriate fluid, the temperature gradient can generate a corresponding gradient in the electrophoretic velocity so that the electrophoretic and bulk velocities sum to zero at a unique position along the conduit and the material will be focused at that position. The method and device may be adapted for use with a variety of materials including fluorescent dyes, amino acids, proteins, DNA and to concentrate a dilute material.Type: GrantFiled: January 24, 2005Date of Patent: August 11, 2009Assignee: The United States of America as represented by the Secretary of Commerce, the National Institute of Standards and TechnologyInventors: David J. Ross, Wyatt N. Vreeland, Karin M. Balss
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Patent number: 7494577Abstract: A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte.Type: GrantFiled: September 30, 2003Date of Patent: February 24, 2009Assignee: Monogram Biosciences, Inc.Inventors: Stephen J. Williams, Hong Dong Tan, Hung Pin Kao, Wyatt N. Vreeland
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Publication number: 20080227211Abstract: The present invention provides compositions and methods for performing free-solution conjugate analysis of nucleic acid molecules. For example, the present invention provides multiplexed single-base extension assays for genotyping. In particular, the present invention provides a series of disperse polyamide “drag tags” for use in achieving high-resolution separation of nucleic acid reaction products.Type: ApplicationFiled: December 19, 2007Publication date: September 18, 2008Applicant: Northwestern UniversityInventors: Robert J. Meagher, Wyatt N. Vreeland, Annelise E. Barron
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Patent number: 6818113Abstract: A method and device for injecting a liquid sample into an electrolyte channel in a microfluidics device is disclosed. The device has a channel network that includes an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that intersect the electrolyte channel between the two channel portions at first, second, and third ports, respectively. In the method, a sample is moved electrokinetically into the electrolyte channel, to form a defined sample volume therein. By simultaneously controlling the voltage applied to the three side channels, and at least one of the upstream and downstream channel end portions, the sample volume element can be shaped to have a desired leading- and trailing-edge shape and/or distribution of sample components within the volume elements.Type: GrantFiled: February 10, 2001Date of Patent: November 16, 2004Assignee: Aclara Biosciences, Inc.Inventors: Stephen J. Williams, Hong Dong Tan, Hung Pin Kao, Wyatt N. Vreeland
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Publication number: 20040206626Abstract: A method and device are provided for affinity gradient focusing for directing at least one analyte in a solution containing a pseudostationary phase and located in a channel such as a capillary or a microchannel. The method includes establishing a steady-state spatial gradient in a retention factor of the pseudostationary phase for the at least one analyte. The analyte is caused to be moved within the channel whereby the concentration of the at least one analyte changes at one or more positions along the gradient. The pseudostationary phase is charged and the analyte is either neutral or charged or alternatively, the pseudostationary phase is neutral and the analyte is charged. The device may include a fluid channel, a pseudostationary phase having a retention factor gradient, an electrical current source and a pump system for establishing the bulk flow in the solution in the channel.Type: ApplicationFiled: June 10, 2004Publication date: October 21, 2004Inventors: David J. Ross, Peter B. Howell, Wyatt N. Vreeland
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Publication number: 20040060821Abstract: A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte.Type: ApplicationFiled: September 30, 2003Publication date: April 1, 2004Applicant: ACLARA BioSciences, Inc.Inventors: Stephen J. Williams, Hong Dong Tan, Hung Pin Kao, Wyatt N. Vreeland
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Patent number: 6685813Abstract: A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte.Type: GrantFiled: August 20, 2001Date of Patent: February 3, 2004Assignee: Aclara Biosciences, Inc.Inventors: Stephen J. Williams, Hong Dong Tan, Hung Pin Kao, Wyatt N. Vreeland
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Publication number: 20020079223Abstract: A method of separating components having a given negative or positive charge and contained in a sample is disclosed. The method involves, in one embodiment, loading a microchannel with a sample, placed between a trailing-edge electrolyte having a selected concentration of a titratable species, and a leading-edge electrolyte.Type: ApplicationFiled: August 20, 2001Publication date: June 27, 2002Inventors: Stephen J. Williams, Hong Dong Tan, Hung Pin Kao, Wyatt N. Vreeland
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Publication number: 20020008029Abstract: A method and device for injecting a liquid sample into an electrolyte channel in a microfluidics device is disclosed. The device has a channel network that includes an electrolyte channel having upstream and downstream channel portions and first, second, and third side channels that intersect the electrolyte channel between the two channel portions at first, second, and third ports, respectively. In the method, a sample is moved electrokinetically into the electrolyte channel, to form a defined sample volume therein. By simultaneously controlling the voltage applied to the three side channels, and at least one of the upstream and downstream channel end portions, the sample volume element can be shaped to have a desired leading- and trailing-edge shape and/or distribution of sample components within the volume elements.Type: ApplicationFiled: February 10, 2001Publication date: January 24, 2002Inventors: Stephen J. Williams, Hong Dong Tan, Hung Pin Kao, Wyatt N. Vreeland