Patents by Inventor Xavier von Hatten
Xavier von Hatten has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240150508Abstract: In some examples, novel photochemically-reversible hydrogels and nanogel particles are described comprising copolymer chains including at least one reactive alkene or reactive 1,4-diene end group capable of [2+2] or [2+2+2+2] photodimerization, respectively, at wavelengths >270 nm. In various examples, the photochemically-reversible hydrogels comprise copolymer chains including at least one —N3, —C?CH or —CO2H end group for dual functionality and/or pH responsiveness. For nucleic acid sequencing, amplification primers are grafted to photochemically-reversible hydrogels or nanogel particles reversibly bound to surfaces within a flow cell. After sequencing is complete, the photochemically-reversible hydrogel or nanogel particles is/are removable from the flow cell surfaces by irradiation, enabling the flow cell to be reusable.Type: ApplicationFiled: October 6, 2023Publication date: May 9, 2024Applicants: Illumina, Inc., Illumina Cambridge Limited, Illumina Singapore Pte. Ltd.Inventors: Nam Nguyen, Xavier von Hatten, Wayne George, Gianluca Artioli, Brian Mather, Johan Basuki, Shima Gholizadeh
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Publication number: 20240124916Abstract: The present disclosure relates to a nanoparticle including a first layer including a first polymer and a first plurality of accessory oligonucleotides, a second layer including a second polymer and a single template site for bonding a template polynucleotide, and a third layer including a third polymer and a second plurality of accessory oligonucleotides. Also described herein is a method of making said nanoparticle, including “dip-coating,” e.g., successively dipping a surface with wettable nanodomains in different polymer solutions. Further described herein is a method of making the nanoparticles by forming them in nanowells and subsequently releasing them from the nanowells. Also described herein is a method of attaching the nanoparticle to a substrate and amplifying the template polynucleotide using a polymerase.Type: ApplicationFiled: September 15, 2023Publication date: April 18, 2024Applicant: ILLUMINA CAMBRIDGE LIMITEDInventors: Alexandra SZEMJONOV, Angelo LA ROSA, Gianluca ARTIOLI, Xavier VON HATTEN, Alexandre RICHEZ
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Publication number: 20240117426Abstract: In some examples, novel nanogel particles are described having dual functionality, temperature responsiveness and pH responsiveness. For nucleic acid sequencing, amplification primers are grafted to nanogel particles to form primer-grafted nanogel particles, and the primer-grafted nanogel particles are captured onto surfaces within a flow cell. Within flow cells such as used in SBS nucleic acid sequencing, each primer-grafted nanogel particle functions as a nano-well in the flow cell, thus eliminating the need for nano-wells in some examples.Type: ApplicationFiled: September 18, 2023Publication date: April 11, 2024Applicants: Illumina, Inc., Illumina Cambridge LimitedInventors: Nam Nguyen, Xavier von Hatten, Will Tovey, Andrew Brown, Wayne George, Eric Brustad, Gianluca Artioli
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Publication number: 20230416435Abstract: A co-polymer includes a plurality of a first monomer including a terminal functional group that is to attach to at least two different primers; a plurality of a second monomer including a second functional group that is different from the terminal functional group, and that is selected from the group consisting of a phenyl group, methoxy propyl, glycosyl, vinyl pyrrolidone, and an imidazole group; and a plurality of a third monomer that is different from the first and second monomers. This co-polymer may be used in a flow cell, and may enhance the clustering efficiency and kinetics.Type: ApplicationFiled: June 6, 2023Publication date: December 28, 2023Inventors: Gianluca Andrea Artioli, Timothy J.N. Beech, Mathieu Lessard-Viger, Rebecca Turk-MacLeod, Brian D. Mather, Weixian Xi, Xavier von Hatten
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Publication number: 20230348970Abstract: A functionalized nanostructure includes a metal nanostructure; an un-cleavable first primer and a cleavable second primer attached to a first region of the metal nanostructure through i) a first thiol linkage attached to a first polymer chain having a first polarity or ii) respective first thiol linkages attached to respective first polymer chains having the first polarity; and a cleavable first primer and an un-cleavable second primer attached to a second region of the metal nanostructure through i) a second thiol linkage attached to a second polymer chain having a second polarity different from the first polarity or ii) respective second thiol linkages attached to respective second polymer chains having the second polarity.Type: ApplicationFiled: March 1, 2023Publication date: November 2, 2023Inventors: Gianluca Andrea Artioli, Luca Redivo, Alexandre Richez, Xavier von Hatten
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Publication number: 20230272469Abstract: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.Type: ApplicationFiled: May 9, 2023Publication date: August 31, 2023Inventors: Sean M. Ramirez, Brian D. Mather, Edwin Li, Sojeong Moon, Innsu Daniel Kim, Alexandre Richez, Ludovic Vincent, Xavier von Hatten, Hai Quang Tran, Maxwell Zimmerley, Julia Morrison, Gianluca Andrea Artioli, Krystal Sly, Hayden Black, Lewis J. Kraft, Hong Xie, Wei Wei, Ryan Sanford
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Patent number: 11733147Abstract: In an example, a flow cell includes a substrate, a selectively removable porous molecular network on the substrate and defining exposed substrate regions, and sequencing surface chemistry on at least some of the exposed regions. The sequencing surface chemistry is selected from the group consisting of i) an activated pad, a polymer layer attached to the activated pad, and a primer attached to the polymer layer; or ii) a nanostructure and an enzyme attached to the nanostructure.Type: GrantFiled: July 25, 2019Date of Patent: August 22, 2023Assignee: Illumina Cambridge LimitedInventor: Xavier von Hatten
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Patent number: 11667969Abstract: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.Type: GrantFiled: May 2, 2018Date of Patent: June 6, 2023Assignees: Illumina, Inc., Illumina Cambridge Limited, Illumina Singapore Pte. Ltd.Inventors: Sean M. Ramirez, Brian D. Mather, Edwin Li, Sojeong Moon, Innsu Daniel Kim, Alexandre Richez, Ludovic Vincent, Xavier von Hatten, Hai Quang Tran, Maxwell Zimmerley, Julia Morrison, Gianluca Andrea Artioli, Krystal Sly, Hayden Black, Lewis J. Kraft, Hong Xie, Wei Wei, Ryan Sanford
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Publication number: 20220364977Abstract: An example method includes introducing a first fluid to a flow channel of a flow cell including a working electrode having a surface that is at least partially exposed to the flow channel, the surface being unmodified or modified with a first member of a transition metal complex binding pair, whereby a linking moiety of a complex present in the first fluid chemically attaches the complex to the surface to form a temporarily modified surface of the working electrode; performing a sensing operation involving the complex of the temporarily modified surface; and applying a desorption voltage of the linking moiety to the working electrode, thereby detaching the linking moiety and regenerating the surface.Type: ApplicationFiled: April 22, 2022Publication date: November 17, 2022Inventors: Hayden Black, Xavier von Hatten
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Patent number: 11447582Abstract: Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications.Type: GrantFiled: January 10, 2020Date of Patent: September 20, 2022Assignee: Illumina Cambridge LimitedInventors: Andrew A. Brown, Wayne N. George, Alexandre Richez, Anne-Cecile Dingwall, Xavier von Hatten
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Patent number: 11390864Abstract: In the examples set forth herein, nucleic acid extraction materials are capable of selectively extracting cell free nucleic acids, including cell free DNA, directly from whole blood samples or plasma. Also included are methods of making and using the nucleic acid extraction materials. One example of a nucleic acid extraction material includes a substrate. This example of the nucleic acid extraction material also includes a polycation bonded to at least a portion of a surface of the substrate. In this example, the polycation consists of a polymer of a quaternized monomer selected from the group consisting of a quaternized 1-vinylimidazole monomer and a quaternized dimethylaminoethyl methacrylate monomer, or a copolymer of a neutral monomer and the quaternized monomer.Type: GrantFiled: June 28, 2018Date of Patent: July 19, 2022Assignees: Illumina, Inc., Illumina Cambridge LimitedInventors: Brian D. Mather, Cyril Delattre, Tarun Kumar Khurana, Yir-Shyuan Wu, Pallavi Daggumati, Behnam Javanmardi, Filiz Gorpe-Yasar, Sebastien Georg Gabriel Ricoult, Xavier von Hatten, Daniel Leonard Fuller
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Publication number: 20220185927Abstract: A hydrogel includes a dendritic core with 2 to 30 arms, and first and second acrylamide monomers incorporated into each arm. The first acrylamide monomer is: (I), wherein R1 and R2 are independently selected from an alkyl, an alkylamino, an alkylamido, an alkylthio, an aryl, a glycol, and optionally substituted variants thereof; and the second acrylamide monomer is: (II), wherein R3 and R4 are independently hydrogen or an alkyl; L is a linker including a linear chain of 2 to 20 atoms selected from carbon, oxygen, and nitrogen and optional substituents on the carbon and any nitrogen atoms; A is an N substituted amide: (III), where R5 is hydrogen or an alkyl; E is a linear chain of 1 to 4 atom(s) selected from carbon, oxygen and nitrogen, and optional substituents on the carbon and any nitrogen atoms; and Z is an optional nitrogen containing heterocycle.Type: ApplicationFiled: December 1, 2020Publication date: June 16, 2022Inventors: Gianluca Andrea Artioli, Andrew A. Brown, Wayne N. George, Colin Pilkington, Jem Pitcairn, Alexandre Richez, Xavier von Hatten
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Publication number: 20220154273Abstract: An example of an incorporation mix includes a liquid carrier, a complex, and a labeled nucleotide. The complex includes a polymerase and a plasmonic nanostructure linked to the polymerase. The labeled nucleotide includes a nucleotide, a 3? OH blocking group attached to a sugar of the nucleotide, and a dye label attached to a base of the nucleotide.Type: ApplicationFiled: November 12, 2021Publication date: May 19, 2022Inventors: Gianluca Andrea Artioli, Mathieu Lessard-Viger, Brian D. Mather, Seth M. McDonald, Kaitlin M. Pugliese, Xavier von Hatten
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Publication number: 20220155211Abstract: In an example method, a hydrogel is applied to a surface of a substrate and primers are grafted to the applied hydrogel. Before or after the primers are grafted, plasmonic nanostructures are introduced to the applied hydrogel. This substrate can make up one surface of a flow cell. When the flow cell is used in a sequencing operation, the plasmonic nanostructures can enhance fluorescent signals that are generated.Type: ApplicationFiled: November 12, 2021Publication date: May 19, 2022Inventors: Gianluca Andrea Artioli, Xavier von Hatten
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Publication number: 20220154177Abstract: An example of a functionalized plasmonic nanostructure includes a plasmonic nanostructure core; a polymeric hydrogel attached to the plasmonic nanostructure core, the polymeric hydrogel having a thickness ranging from about 10 nm to about 200 nm; and a plurality of primers attached to side chains or arms of the polymeric hydrogel, wherein at least some of the plurality of primers are attached to the polymeric hydrogel at different distances from the plasmonic nanostructure core.Type: ApplicationFiled: November 12, 2021Publication date: May 19, 2022Inventors: Gianluca Andrea Artioli, Mathieu Lessard-Viger, Brian D. Mather, Xavier von Hatten
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Patent number: 11199540Abstract: Some examples described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.Type: GrantFiled: June 3, 2020Date of Patent: December 14, 2021Assignee: Illumina Cambridge LimitedInventors: Xavier von Hatten, Wayne N. George, Alexandre Richez, Anne-Cecile Dingwall, Andrew A. Brown
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Patent number: 10906044Abstract: Embodiments of present application are directed to micro fluidic devices and particularly digital micro fluidic devices with improved droplet operations, and methods of improving droplet operations in micro fluidic devices.Type: GrantFiled: August 31, 2016Date of Patent: February 2, 2021Assignee: ILLUMINA CAMBRIDGE LIMITEDInventor: Xavier Von Hatten
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Publication number: 20200325467Abstract: An example of a nucleic acid extraction material includes a substrate. The nucleic acid extraction material also includes a polycation bonded to at least a portion of a surface of the substrate. The polycation consists of a polymer of a quaternized monomer selected from the group consisting of a quaternized 1-vinylimidazole monomer and a quaternized dimethylaminoethyl methacrylate monomer, or a copolymer of a neutral monomer and the quaternized monomer.Type: ApplicationFiled: June 28, 2018Publication date: October 15, 2020Inventors: Brian D. Mather, Cyril Delattre, Tarun Kumar Khurana, Yir-Shyuan Wu, Pallavi Daggumati, Behnam Javanmardi, Filiz Gorpe-Yasar, Sebastien Georg Gabriel Ricoult, Xavier von Hatten, Daniel Leonard Fuller
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Publication number: 20200292540Abstract: Some examples described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.Type: ApplicationFiled: June 3, 2020Publication date: September 17, 2020Inventors: Xavier von Hatten, Wayne N. George, Alexandre Richez, Anne-Cecile Dingwall, Andrew A. Brown
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Patent number: 10684281Abstract: Some embodiments described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.Type: GrantFiled: May 3, 2018Date of Patent: June 16, 2020Assignee: ILLUMINA CAMBRIDGE LIMITEDInventors: Xavier von Hatten, Wayne N. George, Alexandre Richez, Anne-Cecile Dingwall, Andrew A. Brown