Abstract: In one aspect, embodiments disclosed herein are directed to engineered CRISPRCas effector proteins that comprise at least one modification compared to an unmodified CRISPR-Cas effector protein that enhances binding of the of the CRISPR complex to the binding site and/or alters editing preference as compared to wild type. In certain example embodiments, the CRISPR-Cas effector protein is a Type II effector protein. In certain other example embodiments, the Type V effector protein is Cas9 or an orthologs or engineered variant thereof. Example Cas9 proteins suitable for use in the embodiments disclosed herein are discussed in further detail below.

Abstract: Disclosed a method for predicting permeability of a multi-mineral phase digital core based on deep learning. In the present disclosure, a three-dimensional digital core is constructed and a pore structure is randomly generated; after a plurality of multi-mineral digital core images is acquired by performing image segmentation on the three-dimensional digital core, permeability corresponding to each of the multi-mineral digital core images is acquired by simulation using multi-physics field simulation software and a multi-mineral digital core data set is constructed based on the plurality of multi-mineral digital core images and the permeability corresponding to each of the multi-mineral digital core images; an SE-ResNet18 convolutional neural network is constructed and trained with the multi-mineral digital core data set; and an image of a multi-mineral core to be predicted is input into the trained SE-ResNet18 convolutional neural network to determine the permeability of the multi-mineral core.

Abstract: Embodiments disclosed herein are directed to engineered CRISPR-Cas effector proteins that comprise at least one modification compared to an unmodified CRISPR-Cas effector protein that enhances binding of the CRISPR complex to the binding site and/or alters editing preference as compared to wild type. In certain example embodiments, the CRISPR-Cas effector protein is a Type V effector protein. In certain other example embodiments, the Type V effector protein is Cpf1. Embodiments disclosed herein are directed to viral vectors for delivery of CRISPR-Cas effector proteins, including Cpf1. In certain example embodiments, the vectors are designed so as to allow packaging of the CRISPR-Cas effector protein within a single vector. There is also an increased interest in the design of compact promoters for packing and thus expressing larger transgenes for targeted delivery and tissue-specificity.

Abstract: The invention discloses a charging state analysis method of a electric vehicle based on electrical characteristic sequence analysis. The method includes following steps: step S1, obtaining voltage sampling data and current sampling data of the electric vehicle during charging; step S2, setting a time interval, so as to divide the voltage sample data and the current sampling data obtained in step S1 into multiple data sets; step S3, calculating an electrical characteristic vector of each time interval; step S4. adding the calculation results of Step S3 to a temperature sensing value T, and generate an electrical characteristic sequence of whole charging cycle; step S5, inputting the electrical characteristic sequence of the electric vehicle into a trained Time Recurrent Neural Network (TRNN) in sequence to obtain corresponding results; if the result is 1, it means normal; if the result is 0, it means abnormal.

Abstract: Embodiments disclosed herein are directed to engineered CRISPR-Cas effector proteins that comprise at least one modification compared to an unmodified CRISPR-Cas effector protein that enhances binding of the of the CRISPR complex to the binding site and/or alters editing preference as compared to wild type. In certain example embodiments, the CRISPR-Cas effector protein is a Type V effector protein. In certain other example embodiments, the Type V effector protein is Cpf1. Embodiments disclosed herein are directed to viral vectors for delivery of CRISPR-Cas effector proteins, including Cpf1. In certain example embodiments, the vectors are designed so as to allow packaging of the CRISPR-Cas effector protein within a single vector. There is also an increased interest in the design of compact promoters for packing and thus expressing larger transgenes for targeted delivery and tissue-specificity.

Abstract: A method for programming the energy storage device in power-gas coupling system based on reliability constraints is provided. The method includes: obtain the parameters and operation condition of each equipment of the power-gas coupling system in a year; determine the different operating states of the system. A programming model of the energy storage device based on reliability constraints is constructed based on the operating state of the system, and benders decomposition algorithm is adopted to calculate the programming model, so that the programming scheme of the energy storage device is obtained. Considering not only the economy but also the reliability of the system, which is more accurate, comprehensive and effective than the previous programming method; the present invention is of great significance to improve the reliability of the electric power system and ensure the safe and reliable operation of the electric power system.

Abstract: A non-intrusive load identification method based on the Power Fingerprint characteristics of the load is provided. The method includes: S1, collecting Power Fingerprint characteristic data of several loads of the same type; S2, after preprocessing Power Fingerprint characteristic data of load, establishing convolution neural network based on attention mechanism to learn load characteristics; S3, using sliding time window algorithm to realize load switching event detection, In order to extract the change of electrical data of user bus before and after the switching event, the non-intrusive load identification problem is converted into the single load identification problem; S4, the load identification is realized, and the extracted electrical information features of single load are identified using the trained model.

Abstract: The present invention relates to an automatic history matching system for an oil reservoir based on transfer learning, comprising a data reading module, a population reinitializing module, an optimization module, a simulated calculation module, a comparative judgment module and an output module, wherein the data reading module reads an optimized result of an existing oil reservoir, outputs the optimized result to the population reinitializing module, obtains an initial population of a new oil reservoir by calculation and outputs the initial population to the optimization module; the optimized result is outputted to the simulated calculation module to obtain oil reservoir production simulated data, and the oil reservoir production simulated data is outputted to the comparative judgment module; when an error between the simulated data and observed data meets the requirement, the optimized result is outputted to the output module, and the system operation is completed; and if the error does not meet the requirem

Abstract: Embodiments disclosed herein are directed to engineered CRISPR-Cas effector proteins that comprise at least one modification compared to an unmodified CRISPR-Cas effector protein that enhances binding of the of the CRISPR complex to the binding site and/or alters editing preference as compared to wild type. In certain example embodiments, the CRISPR-Cas effector protein is a Type V effector protein. In certain other example embodiments, the Type V effector protein is Cpf1. Embodiments disclosed herein are directed to viral vectors for delivery of CRISPR-Cas effector proteins, including Cpf1. In certain example embodiments, the vectors are designed so as to allow packaging of the CRISPR-Cas effector protein within a single vector. There is also an increased interest in the design of compact promoters for packing and thus expressing larger transgenes for targeted delivery and tissue-specificity.

Abstract: The present invention discloses a method for programming the energy storage device in power-gas coupling system based on reliability constraints, obtain the parameters and operation condition of each equipment of the power-gas coupling system in a year; determine the different operating states of the system; A programming model of the energy storage device based on reliability constraints is constructed based on the operating state of the system, and benders decomposition algorithm is adopted to calculate the programming model, so that the programming scheme of the energy storage device is obtained. Considering not only the economy but also the reliability of the system, which is more accurate, comprehensive and effective than the previous programming method; the present invention is of great significance to improve the reliability of the electric power system and ensure the safe and reliable operation of the electric power system.

Abstract: In one aspect, embodiments disclosed herein are directed to engineered CRISPR-Cas effector proteins that comprise at least one modification compared to an unmodified CRISPR-Cas effector protein that enhances binding of the of the CRISPR complex to the binding site and/or alters editing preference as compared to wild type. In certain example embodiments, the CRISPR-Cas effector proteins a Type II effector protein. In certain other example embodiments, the Type V effector protein is Cas9 or an orthologs or engineered variant thereof. Example Cas9 proteins suitable for use in the embodiments disclosed herein are discussed in further detail below.

Abstract: Provided herein are methods of detecting a nucleic acid modification, methods for detecting off-target activity of a targeted nuclease specific for a selected target sequence, methods for determining cleavage efficiency of a targeted nuclease specific for a selected target sequence, methods for selecting a guide RNA from a plurality of guide RNAs specific for a selected target sequence, methods for enrichment of one or more nucleic acid molecules wherein a nucleic acid modification is made and kits of parts for use in such methods.

Abstract: Embodiments disclosed herein are directed to engineered CRISPR-Cas effector proteins that comprise at least one modification compared to an unmodified CRISPR-Cas effector protein that enhances binding of the of the CRISPR complex to the binding site and/or alters editing preference as compared to wild type. In certain example embodiments, the CRISPR-Cas effector protein is a Type V effector protein. In certain other example embodiments, the Type V effector protein is Cpf1. Embodiments disclosed herein are directed to viral vectors for delivery of CRISPR-Cas effector proteins, including Cpf1. In certain example embodiments, the vectors are designed so as to allow packaging of the CRISPR-Cas effector protein within a single vector. There is also an increased interest in the design of compact promoters for packing and thus expressing larger transgenes for targeted delivery and tissue-specificity.

Abstract: Disclosed are a halogen-free fire-retardant polyamide composition, and a preparation method and application thereof. The halogen-free fire-retardant polyamide composition is prepared from 35 to 71.5% of a semi-aromatic polyamide, 10 to 35% of a fire retardant and 0 to 50% of an inorganic reinforcing filler, the terminal amino group content of the semi-aromatic polyamide being 80 to 150 mol/t. The halogen-free fire-retardant polyamide composition of the present invention has good heat stability and releases only a small amount of gas in production; mold dirt is not easily formed, and the halogen-free fire-retardant polyamide composition can be produced continuously.

Abstract: The present invention discloses a heat-resistant polyamide composition and application thereof. The composition comprises the following components in percentage by weight: 40% to 90% of heat-resistant polyamide resin, 5% to 35% of mineral fiber A, 0 to 35% of mineral filler B, 0.1% to 1% of light stabilizer, 0.1% to 1% of flow modifier and 0.1% to 1% of antioxidant. In the present invention, heat-resistant polyamide resin with the ratio of amine-terminated group and carboxyl-terminated group between 0.1 and 0.8 is selected to be matched with deformed glass fibers with an aspect ratio of 2 to 6, the mineral filler B and the flow modifier to obtain the heat-resistant polyamide composition.

Abstract: Disclosed are a halogen-free fire-retardant polyamide composition, and a preparation method and application thereof. The halogen-free fire-retardant polyamide composition is prepared from 35 to 71.5% of a semi-aromatic polyamide, 10 to 35% of a fire retardant and 0 to 50% of an inorganic reinforcing filler, the terminal amino group content of the semi-aromatic polyamide being 80 to 150 mol/t. The halogen-free fire-retardant polyamide composition of the present invention has good heat stability and releases only a small amount of gas in production; mold dirt is not easily formed, and the halogen-free fire-retardant polyamide composition can be produced continuously.

Abstract: The present invention discloses a heat-resistant polyamide composition and application thereof. The composition comprises the following components in percentage by weight: 40% to 90% of heat-resistant polyamide resin, 5% to 35% of mineral fiber A, 0 to 35% of mineral filler B, 0.1% to I% of light stabilizer, 0.1% to 1% of flow modifier and 0.1% to 1% of antioxidant. In the present invention, heat-resistant polyamide resin with the ratio of amine-terminated group and carboxyl-terminated group between 0.1 and 0.8 is selected to be matched with deformed glass fibers with an aspect ratio of 2 to 6, the mineral filler B and the flow modifier to obtain the heat-resistant polyamide composition.

Abstract: A socket protector for fixing a protective cover to a housing includes a connecting member and a receiving groove defined in the housing. The connecting member is connected to the protective cover. The connecting member has an elastic connecting portion defining a restricting hole. A restricting portion is formed in the receiving groove. When the elastic connecting portion resists the restricting portion, the elastic connecting portion deforms to extend the restricting portion through the restricting hole of the elastic connecting portion, thus connecting the protective cover to the housing.

Abstract: A socket protector for fixing a protective cover to a housing includes a connecting member and a receiving groove defined in the housing. The connecting member is connected to the protective cover. The connecting member has an elastic connecting portion defining a restricting hole. A restricting portion is formed in the receiving groove. When the elastic connecting portion resists the restricting portion, the elastic connecting portion deforms to extend the restricting portion through the restricting hole of the elastic connecting portion, thus connecting the protective cover to the housing.