Abstract: This invention provides a process for sequencing single-stranded DNA employing modified nucleotides.

Abstract: Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing of a nucleic acid.

Abstract: The present application discloses novel and inventive methods, apparatuses, and the required molecules to detect compounds (analytes) utilizing antibodies or other protein-binding molecules and nanopore-detectable tags using specially designed nanopores, coupled with ionic current readout, to provide a single molecule electronic detection solution, offering unique opportunities for multiplexing and quantitation.

Abstract: The invention provides various orthogonal nucleotide analogues and methods for using combinations of said various orthogonal nucleotide analogues for sequencing by synthesis.

Abstract: This invention provides a process for sequencing single-stranded DNA employing modified nucleotides.

Abstract: Disclosed herein, inter alia, are compounds, compositions, and methods of thereof in the sequencing a nucleic acid.

Abstract: The subject invention provides a method of determining whether a cytosine at a predefined position within a single strand of a double-stranded DNA of known sequence is hydroxymethylated. The invention also provides a method of determining whether a cytosine at a predefined position within a single strand of a double-stranded DNA of known sequence is unmethylated. The invention further provides a method of determining whether a cytosine at a predefined position within a single strand of a double-stranded DNA of known sequence is methylated but not hydroxymethylated. The invention also provides a method of determining whether a cytosine present at a predefined position within a single strand of a double-stranded DNA of known sequence, and within a CpG site, is unmethylated.

Abstract: The present invention provides a method of determining whether cytosine residues present at a predetermined positions within a single strand of a double stranded DNA of known sequence are methylated as well as compounds for carrying out this method.

Abstract: This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-stranded

Abstract: This invention provides a process for sequencing single-stranded DNA employing modified nucleotides.

Abstract: This invention provides nucleoside polyphosphate analogues each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs. This invention also provides nucleotide polymerases with one or more attached and/or conjugated noble metal nanoparticles, wherein the noble metal nanoparticles are surface-enhanced Raman spectroscopy (SERS) substrates thereby creating a region of enhanced sensitivity for surface enhanced Raman spectroscopy (SERS) within or adjacent to the polymerase. This invention also provides a surface with regions of enhanced sensitivity for surface enhanced Raman spectroscopy comprising interspersed rough or nanostructured noble metal surface. This invention also provides methods for determining the sequence of a single stranded DNA or RNA polynucleotide using one or more of nucleoside polyphosphate analogues, polymerase with noble metal nanoparticles, and surface with noble metal.

Abstract: A method for abandonment of a subsea well includes: setting a packer of a lower cementing tool against a bore of an inner casing hung from a subsea wellhead; fastening a pressure control assembly (PCA) to the subsea wellhead; hanging an upper cementing tool from the PCA and stabbing the upper cementing tool into a polished bore receptacle of the lower cementing tool; perforating a wall of the inner casing below the packer; perforating the inner casing wall above the packer by operating a perforator of the upper cementing tool; mixing a resin and a hardener to form a sealant; and pumping a fluid train through bores of the cementing tools and into an inner annulus formed between the inner casing and an outer casing hung from the subsea wellhead. The fluid train includes the sealant followed by a cement slurry.

Abstract: This disclosure is related to a method for determining the identity of a nucleotide residue of a single-stranded DNA or RNA, or sequencing DNA or RNA, in a solution using an ion-sensing field effect transistor and reversible nucleotide terminators.

Abstract: Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing of a nucleic acid.

Abstract: This invention provides a process for sequencing single-stranded DNA employing modified nucleotides.

Abstract: This invention provides methods of derivatizing a double-stranded DNA comprising contacting double-stranded DNA with a CpG methyltransferase and an s-adenosylmethionine analog. This invention also provides methods of sequencing DNA to determine methylation patterns.

Abstract: This invention provides a process for sequencing single-stranded DNA employing modified nucleotides.

Abstract: A method for abandonment of a subsea well includes: setting a packer of a lower cementing tool against a bore of an inner casing hung from a subsea wellhead; fastening a pressure control assembly (PCA) to the subsea wellhead; hanging an upper cementing tool from the PCA and stabbing the upper cementing tool into a polished bore receptacle of the lower cementing tool; perforating a wall of the inner casing below the packer; perforating the inner casing wall above the packer by operating a perforator of the upper cementing tool; mixing a resin and a hardener to form a sealant; and pumping a fluid train through bores of the cementing tools and into an inner annulus formed between the inner casing and an outer casing hung from the subsea wellhead. The fluid train includes the sealant followed by a cement slurry.

Abstract: This invention provides a process for sequencing single-stranded DNA employing modified nucleotides.

Abstract: The present invention provides a method of determining whether cytosine residues present at a predetermined positions within a single strand of a double stranded DNA of known sequence are methylated as well as compounds for carrying out this method.