Abstract: The disclosure discloses an ultrasonic-assisted pretreatment method for extraction of multiple steroid hormones in a sediment, including the following steps: (1) lyophilizing the sediment, grinding the sediment, and passing the ground sediment through a 40-60-mesh sieve; (2) placing the sample obtained in step (1) in a container; (3) adding an extractant to the container in step (2), shaking the mixture for 15 s-30 s, centrifuging the mixture to collect an supernatant after ultrasonication, and repeating extraction three times; where the extractants used in the three times of extraction are two of methanol, acetonitrile and acetone; and (4) after mixing the supernatants of the three times of extraction obtained in step (3), concentrating the mixture under a nitrogen flow at 20-30° C., passing the concentrated mixture through a filter, and performing detection. By using the method of the disclosure, the maximum recovery can be up to 100%.

Abstract: Disclosed herein are high Tm RNA nanostructures that can be composed of one or more modules or motifs to build RNA nanostructures with or without layers. The RNA nanostructures can have a core domain and three or more double-stranded arms and formulations thereof to conjugate high copy numbers of therapeutics, pH responsive or enzyme cleavable drug cargo. Also described herein is a design strategy for generation of synthetic RNA oligonucleotides that can self assemble into highly thermostable RNA structures. Also described herein are uses of the RNA nanostructures described herein.

Abstract: An antenna device includes a plurality of dipole antennas and a port. Each of the dipole antennas is connected to the port. The dipole antennas are arranged around the port. Each of the dipole antennas comprises two ends. The device further includes a plurality of passive elements. The ends of the dipole antennas and the passive elements are interchangeably arranged around the port such that each of the passive elements is situated between ends of two different antennas from the plurality of dipole antennas. One or more switches are configured to switch between an omnidirectional state, in which the ends of the dipole antennas are not connected to the plurality of passive elements, and a directional state, in which at least one end of one of the passive elements is connected to at least one end of one of the antennas.

Abstract: The present invention relates to adeno-associated viral (AAV) particles encoding siRNA molecules and methods for treating Huntington's Disease (HD).

Abstract: A microcapsule type polycarboxylate superplasticizer and a preparation method thereof. The microcapsule type polycarboxylate superplasticizer is a polycarboxylic acid aqueous solution encapsulated by a closed capsule, and the closed capsule is an organic/inorganic composite shell layer of calcium ion gel of sodium alginate or gelatin, and calcium carbonate. The organic/inorganic composite shell layer is obtained through diffusion-interface reaction of sodium alginate or gelatin, calcium salt and urea under the catalysis of urease. The microcapsule type polycarboxylate superplasticizer meets requirement of long-term and stable slump retaining of concrete in some special engineering. The preparation method has simple processes, free of organic solvent, and has high encapsulating efficiency.

Abstract: Providing a multi-functional group superplasticizer for an ultra-high performance concrete and a method for preparing the same. Its backbone is an alkyl chain, and its side chain are some side chains with carboxylic acid or carboxylate at terminals, some polyether side chains, and some polyol amine side chains substituted with phosphoric acid or phosphite at terminals, the polyol amine side chains substituted with phosphoric acid or phosphite at terminals is connected to the backbone through a phenyl or an alkyl group of 1-9 carbons, and a ratio of a number of the side chains with carboxylic acid or carboxylate at terminals to a total number of side chains is ?0 and ?0.8; and a ratio of a number of the polyether side chains to the total number of side chains is ?0.1 and ?0.9.

Abstract: The present application discloses a polyhydroxy aromatic intermediate, preparation thereof and use thereof in a polycondensate water-reducer with branched side chains. The polycondensate water-reducer with branched side chains has a branched side chain structure which provides a stronger steric hindrance. The synergistic effect of the branched side chains and the rigid skeleton of the aromatic ring greatly improves the water-reducing ability. Especially under a condition of low water/cement ratio, the improvement in water-reducing ability is more obvious. The branched polyether side chain is more conducive to the formation of a thicker water film layer, which has an obvious viscosity reduction effect. The conformation of the branched polyether side chain is less affected by different ionic environments in the pore solution in cement, and thus has a stronger adaptability to various raw materials.

Abstract: Provided are compositions, methods, kits, and articles of manufacture for use in RNA sequencing. The approach is referred to as easy RNA-adenylation sequencing (“Ezra-seq”). The approach provides an alternative to 3? end linker ligation and circularization by way of an enzymatic system capable of 3? end poly(A) tailing and 5?-end adenylation for the same RNA, using two separate enzymes, or a single fusion protein. The two enzymes or the fusion protein containing them as distinct segments are a cyclase and a polymerase.

Abstract: Disclosed is a preparation method of a polycarboxylate water reducer having a regular sequence structure. The preparation method of the present invention comprises: performing Michael addition on polyethylene glycol ether acrylate macromonomer A and amino-containing carboxylic acid B for 5-12 hours, then cooling to 0° C., dropwise adding acryloyl chloride slowly and evenly, and further reacting for 12 hours at room temperature to obtain carboxyl-containing polyether macromonomer C; mixing the carboxyl-containing polyether macromonomer C, a small molecular RAFT reagent, an initiator, and water adequately; and holding the polymerization mass concentration at 30%-60% and heating to 60-80° C. in the atmosphere of N2 to react for 2-5 hours to obtain the polycarboxylate water reducer having a regular sequence structure.

Abstract: The subject invention pertains to methods and systems for classifying leukocytes using artificial intelligence called AIRFIHA (artificial-intelligence enabled reagent-free imaging hematology analyzer) that can accurately classify subpopulations of leukocytes in a label-free manner. AIRFIHA can not only subtype lymphocytes into B and T cell but is capable of sorting different types of T cells subtypes. AIRFIHA is realized through training a two-step neural network using label-free images of separated leukocytes acquired from a custom-built quantitative phase microscope. Owing to its easy operation, low cost, and strong discerning capability of complex leukocyte subpopulations, AIRFIHA is clinically translatable and can also be deployed in resource-limited settings.

Abstract: The invention relates to a serpentine inspection robot mechanism for lifting cage guide driven by magnetic wheels, comprising a magnetic wheel drive part and a pitching yawing part, wherein the magnetic wheel drive part comprises a worm and gear transmission mechanism (I) and a magnetic wheel driving mechanism (II), and the pitching yawing part comprises a pitching yawing mechanism (III). Magnetic wheels are driven by the worm and gear transmission mechanism (I) and the magnetic wheel driving mechanism (II), while the pitching and yawing control of the robot are realized by the pitching yawing mechanism (III). The serpentine robot driven by the magnetic wheels (22) resolves the problems that the efficiency of ordinary serpentine robot is low, and it is difficult for the simple-wheel-type robot to span the large gap and to reach some concealed corners, with the cage guide of the lifting machine sucked by the magnetic wheels (22).

Abstract: The present invention discloses a polycarboxylic acid water-reducing agent with high adaptability to temperature and a preparation method for preparing the same. The polycarboxylic acid water-reducing agent is prepared by introducing an unsaturated macromonomer D with a temperature-sensitive side chain which could be bonded to the backbone of the polycarboxylic acid through free radical polymerization. The polycarboxylic acid water-reducing agent prepared by the invention has an adsorption group density that can be automatically adjusted with the change of ambient temperature, and thereby shows similar dispersing capability and dispersion retention capability at different ambient temperatures, demonstrating high adaptability to different ambient temperatures. It can be used in a larger temperature range with a constant dosage, which is beneficial to further popularization and application of the polycarboxylic acid water-reducing agent in regions of different climates.

Abstract: The present invention discloses a multitype-adsorptive-group polycarboxylic acid water-reducing agent, consisting of 30-60 wt % of a macromolecule with multitype-adsorptive groups and water, the macromolecule with multitype-adsorptive groups has a polyethylene glycol side chain, and adsorption groups of the polymer backbone include a carboxylic acid group, a sulfonic acid group and a phosphoric acid group, the phosphoric acid group being linked to the backbone of the macromolecule with multitype-adsorptive groups by nucleophilic addition. The water-reducing agent of the present invention has significantly improved adaptability to different cements and aggregates while maintaining a high water reduction efficiency, compared with the conventional polycarboxylic acid water-reducing agent. It has a good water reducing efficiency in many different grades of cement, as well as a better resistance to aggregates containing more impurities such as machine-made sand.

Abstract: Disclosed are a highly adaptable viscosity-reducing polycarboxylate water reducer and its preparation method and application. The highly adaptable viscosity-reducing polycarboxylate water reducer is composed of a polymer and water, and the polymer accounts for 30-50 wt %; since the main chain of the polymer contains a hydrophobic benzene ring and hydrophilic amine, the polymer has strong rigidity and good solubility in an aqueous solution, and is rich in two adsorption groups of carboxyl group/phosphono group. The highly adaptable viscosity-reducing polycarboxylate water reducer of the present invention has excellent water-reducing and slump retention properties and is suitable for general construction projects and commercial concrete projects.

Abstract: Disclosed is an amphiphilic multifunctional hybrid nanoparticle. The nanoparticle of the present invention has a detachable hydrophilic organic polymer with both a water-soluble long chain and a hydrophobic long hydrocarbon functional group attached to the surface, wherein the body of the nanoparticle is a silica or an organofunctional group substituted silica or an organofunctional group substituted silicon-oxygen bond network, and the nanoparticle contains a free organosiloxane with hydrophobic long hydrocarbon functional groups and a fatty acid or fatty acid ester or aluminum complex of fatty acid with hydrophobic long hydrocarbon functional groups.

Abstract: The invention relates to a serpentine inspection robot mechanism for lifting cage guide driven by magnetic wheels, comprising a magnetic wheel drive part and a pitching yawing part, wherein the magnetic wheel drive part comprises a worm and gear transmission mechanism (I) and a magnetic wheel driving mechanism (II), and the pitching yawing part comprises a pitching yawing mechanism (III). Magnetic wheels are driven by the worm and gear transmission mechanism (I) and the magnetic wheel driving mechanism (II), while the pitching and yawing control of the robot are realized by the pitching yawing mechanism (III). The serpentine robot driven by the magnetic wheels (22) resolves the problems that the efficiency of ordinary serpentine robot is low, and it is difficult for the simple-wheel-type robot to span the large gap and to reach some concealed corners, with the cage guide of the lifting machine sucked by the magnetic wheels (22).

Abstract: Disclosed is a preparation method of a polycarboxylate water reducer having a regular sequence structure. The preparation method of the present invention comprises: performing Michael addition on polyethylene glycol ether acrylate macromonomer A and amino-containing carboxylic acid B for 5-12 hours, then cooling to 0° C., dropwise adding acryloyl chloride slowly and evenly, and further reacting for 12 hours at room temperature to obtain carboxyl-containing polyether macromonomer C; mixing the carboxyl-containing polyether macromonomer C, a small molecular RAFT reagent, an initiator, and water adequately; and holding the polymerization mass concentration at 30%-60% and heating to 60-80° C. in the atmosphere of N2 to react for 2-5 hours to obtain the polycarboxylate water reducer having a regular sequence structure.

Abstract: A multiple-state health monitoring apparatus for critical components in a hoisting system includes a frame. The frame is a square structure formed by welding a plurality of rectangular steels. A steel wire rope is arranged around a periphery of the square structure. A power system, a friction-and-wear apparatus, a brake-and-wear apparatus, and a tensioning apparatus are sequentially mounted from left to right on a bottom layer of the square structure. A bearing signal collection system, a tension sensor, an excitation apparatus, and a steel-wire-rope image collection system are sequentially mounted from left to right on a top layer of the square structure. The steel wire rope sequentially passes through all the apparatuses or systems and is driven by the power system to perform circling. All the apparatuses or systems are used to monitor an operation status of the steel wire rope.

Abstract: Embodiments of the present application disclose a method and system for identifying whether an application is genuine by means of digital watermarks, which can conveniently determine whether an application is genuine.

Abstract: A multiple-state health monitoring apparatus for critical components in a hoisting system includes a frame. The frame is a square structure formed by welding a plurality of rectangular steels. A steel wire rope is arranged around a periphery of the square structure. A power system, a friction-and-wear apparatus, a brake-and-wear apparatus, and a tensioning apparatus are sequentially mounted from left to right on a bottom layer of the square structure. A bearing signal collection system, a tension sensor, an excitation apparatus, and a steel-wire-rope image collection system are sequentially mounted from left to right on a top layer of the square structure. The steel wire rope sequentially passes through all the apparatuses or systems and is driven by the power system to perform circling. All the apparatuses or systems are used to monitor an operation status of the steel wire rope.