Patents by Inventor Xingyong Wu
Xingyong Wu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11788124Abstract: The present invention relates to detection of nucleic acids and provides a composition comprising a Signal Generating Complex, wherein the composition comprises: (A) a pair of target probes (TPs), wherein a first TP of the pair of TPs comprises a nucleic acid sequence comprising two segments; (B) a pair of base PPAs comprising the first and second base PPAs, wherein the first base PPA comprises a nucleic acid sequence comprising three segments; (C) a set of extension PPAs comprising the first and second extension PPAs, wherein the first extension PPA comprises a nucleic acid sequence comprising two segments; (D) a plurality of pre-amplifiers (PAs), wherein the PAs comprise a nucleic acid sequence comprising three segments; (E) a plurality of amplifiers (AMPs), wherein the AMPs comprise a nucleic acid sequence comprising two segments; and (F) a plurality of label probes (LPs), wherein the LPs comprise a nucleic acid sequence comprising two segments.Type: GrantFiled: April 8, 2019Date of Patent: October 17, 2023Assignee: ADVANCED CELL DIAGNOSTICS, INC.Inventors: Yuling Luo, Xiao-Jun Ma, Steve Chen, Nan Su, Emerald Doolittle, Bingqing Zhang, Xiaoming Wang, Xingyong Wu, Xiao Yan Pimentel, Helen Jarnagin
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Publication number: 20210395806Abstract: The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites. The method can comprise the steps of contacting the sample with a probe that detects the nucleic acid variation or splice site and a neighbor probe; contacting the sample with pre-amplifiers that bind to the nucleic acid variation probe or splice site probe and neighbor probe, respectively; contacting the sample with a collaboration amplifier that binds to the pre-amplifiers; and contacting the sample with a label probe system, wherein hybridization of the components forms a signal generating complex (SGC) comprising a target nucleic acid with the nucleic acid variation or splice site, the probes and amplifiers; and detecting in situ signal from the SGC on the sample.Type: ApplicationFiled: June 29, 2021Publication date: December 23, 2021Inventors: Yuling Luo, Xingyong Wu, Liuliu Pan, Xiaoming Wang, Xiao-Jun Ma, Nan Su, Steve Chen
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Patent number: 11078528Abstract: The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites. The method can comprise the steps of contacting the sample with a probe that detects the nucleic acid variation or splice site and a neighbor probe; contacting the sample with pre-amplifiers that bind to the nucleic acid variation probe or splice site probe and neighbor probe, respectively; contacting the sample with a collaboration amplifier that binds to the pre-amplifiers; and contacting the sample with a label probe system, wherein hybridization of the components forms a signal generating complex (SGC) comprising a target nucleic acid with the nucleic acid variation or splice site, the probes and amplifiers; and detecting in situ signal from the SGC on the sample.Type: GrantFiled: October 11, 2016Date of Patent: August 3, 2021Assignee: ADVANCED CELL DIAGNOSTICS, INC.Inventors: Yuling Luo, Xingyong Wu, Liuliu Pan, Xiaoming Wang, Xiao-Jun Ma, Nan Su, Steve Chen
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Publication number: 20210032690Abstract: The present invention relates to detection of nucleic acids and provides a composition comprising a Signal Generating Complex, wherein the composition comprises: (A) a pair of target probes (TPs), wherein a first TP of the pair of TPs comprises a nucleic acid sequence comprising two segments; (B) a pair of base PPAs comprising the first and second base PPAs, wherein the first base PPA comprises a nucleic acid sequence comprising three segments; (C) a set of extension PPAs comprising the first and second extension PPAs, wherein the first extension PPA comprises a nucleic acid sequence comprising two segments; (D) a plurality of pre-amplifiers (PAs), wherein the PAs comprise a nucleic acid sequence comprising three segments; (E) a plurality of amplifiers (AMPs), wherein the AMPs comprise a nucleic acid sequence comprising two segments; and (F) a plurality of label probes (LPs), wherein the LPs comprise a nucleic acid sequence comprising two segments.Type: ApplicationFiled: April 8, 2019Publication date: February 4, 2021Inventors: Yuling LUO, Xiao-Jun MA, Steve CHEN, Nan SU, Emerald DOOLITTLE, Bingqing ZHANG, Xiaoming WANG, Xingyong WU, Xiao Yan PIMENTEL, Helen JARNAGIN
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Publication number: 20170101672Abstract: The invention relates to methods of in situ detection of a nucleic acid variation of a target nucleic acid in a sample, including single nucleotide variations, multi-nucleotide variations or splice sites. The method can comprise the steps of contacting the sample with a probe that detects the nucleic acid variation or splice site and a neighbor probe; contacting the sample with pre-amplifiers that bind to the nucleic acid variation probe or splice site probe and neighbor probe, respectively; contacting the sample with a collaboration amplifier that binds to the pre-amplifiers; and contacting the sample with a label probe system, wherein hybridization of the components forms a signal generating complex (SGC) comprising a target nucleic acid with the nucleic acid variation or splice site, the probes and amplifiers; and detecting in situ signal from the SGC on the sample.Type: ApplicationFiled: October 11, 2016Publication date: April 13, 2017Inventors: Yuling Luo, Xingyong Wu, Liuliu Pan, Xiaoming Wang, Xiao-Jun Ma, Nan Su, Steve Chen
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Publication number: 20160201117Abstract: Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of RNAscope® method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of RNAscope® assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.Type: ApplicationFiled: March 17, 2016Publication date: July 14, 2016Inventors: Xingyong WU, Huei-Yu Fay WANG, Nan SU, Li-Chong WANG, Yuling LUO
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Publication number: 20160115555Abstract: Disclosed is a method for diagnosing melanoma in a human subject, as well as a method for providing a prognosis to a human subject who is at risk of developing melanoma recurrence, and a method for determining the stage of melanoma in a human subject, comprising the step of determining the level of expression of phosphatase and actin regulator 1 (PHACTR1) gene, or fragments thereof, either alone or in combination with the level of expression of secreted integrin-binding phosphoprotein (SPP1), preferentially expressed antigen in melanoma (PRAME), growth differentiation factor 15 (GDF15), and chemokine C-X-C motif ligand 10 (CXCL10) genes. Further, the invention relates to a diagnostic kit, comprising at least one substance for detection of the expression of PHACTR1, or fragments thereof, either alone or in combination with the detection of SPP1, PRAME, GDF15, and CXCL10, for the diagnosis or prognosis of melanoma.Type: ApplicationFiled: November 10, 2015Publication date: April 28, 2016Inventors: Xiao-Jun MA, Xingyong WU, Yuling LUO
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Patent number: 9315854Abstract: Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of an in situ hybridization (ISH) assay method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of the ISH assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.Type: GrantFiled: January 9, 2014Date of Patent: April 19, 2016Assignee: Advanced Cell Diagnostics, Inc.Inventors: Xingyong Wu, Huei-Yu Fay Wang, Nan Su, Li-Chong Wang, Yuling Luo
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Publication number: 20140357509Abstract: The invention relates to methods of categorizing a cervical tissue or cytology sample by performing an in situ hybridization assay using an antisense E6 or E7 probe on a cervical tissue sample, wherein the antisense E6 or E7 probe can simultaneously detect HPV DNA and HPV RNA; detecting the presence of HPV nucleic acid; and categorizing the cervical tissue sample based on HPV nucleic acid expression.Type: ApplicationFiled: March 28, 2014Publication date: December 4, 2014Applicant: Advanced Cell Diagnostics, Inc.Inventors: Xiao-Jun MA, Hongwei WANG, Xingyong WU, Yuling LUO
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Publication number: 20140249040Abstract: Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of RNAscope® method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of RNAscope® assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.Type: ApplicationFiled: January 9, 2014Publication date: September 4, 2014Applicant: Advanced Cell Diagnostics, Inc.Inventors: Xingyong WU, Huei-Yu Fay WANG, Nan SU, Li-Chong WANG, Yuling LUO
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Patent number: 8658361Abstract: Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of an in situ hybridization (ISH) assay method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of the ISH assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.Type: GrantFiled: October 21, 2011Date of Patent: February 25, 2014Assignee: Advanced Cell Diagnostics, Inc.Inventors: Xingyong Wu, Huei-Yu Fay Wang, Nan Su, Li-Chong Wang, Yuling Luo
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Publication number: 20120100540Abstract: Disclosed is a method for in situ detection of one or more target nucleic acids based on a combination of RNAscope® method and a general ISH signal amplification method. This new method produces high signal intensity and while keeps low background noise of signal amplification. The result can be consistently reproduced and the method can be easily adopted for routine clinic diagnostic use. Further, the invention relates to a kit, comprising the components of RNAscope® assay and a general ISH signal amplification assay, for sensitive detection of one or more target nucleic acids.Type: ApplicationFiled: October 21, 2011Publication date: April 26, 2012Applicant: Advanced Cell Diagnostics, Inc.Inventors: XINGYONG WU, Huei-Yu Fay WANG, Nan SU, Li-Chong WANG, Yuling LUO
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Publication number: 20120071343Abstract: Disclosed is a method for diagnosing melanoma in a human subject, as well as a method for providing a prognosis to a human subject who is at risk of developing melanoma recurrence, and a method for determining the stage of melanoma in a human subject, comprising the step of determining the level of expression of phosphatase and actin regulator 1 (PHACTR1) gene, or fragments thereof, either alone or in combination with the level of expression of secreted integrin-binding phosphoprotein (SPP1), preferentially expressed antigen in melanoma (PRAME), growth differentiation factor 15 (GDF 15), and chemokine C-X-C motif ligand 10 (CXCL10) genes. Further, the invention relates to a diagnostic kit, comprising at least one substance for detection of the expression of PHACTR1, or fragments thereof, either alone or in combination with the detection of SPP1, PRAME, GDF15, and CXCL10, for the diagnosis or prognosis of melanoma.Type: ApplicationFiled: September 20, 2011Publication date: March 22, 2012Applicant: Advanced Cell Diagnostics, IncInventors: XIAO-JUN MA, Xingyong Wu, Yuling Luo
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Publication number: 20040185495Abstract: This invention provides methods and compositions useful for identifying and diagnosing rare fetal cells in a mixed cell population such as a maternal blood sample. The methods entail the use of specific nucleic acid probes that hybridize to fetal cell associated RNAs to identify the rare fetal cells or antibodies that bind to polypeptides encoded by the fetal cell associated RNAs for fetal cell detection. The cells detected by the methods of the present invention are useful for diagnosing the fetal cells for a genetic trait of interest, such as trisomy 21. Novel methods for simultaneous screening for fetal cells and diagnosing the fetal cells are also provided. Compositions comprising the fetal cell associated nucleic acids of the invention and their encoded proteins are also provided. The present invention further provides kits useful for practicing the present methods.Type: ApplicationFiled: April 5, 2004Publication date: September 23, 2004Inventors: Paula A. Schueler, Hongxia Xu, Lisa Foltz, Xingyong Wu, Yehsiung Sha, Alexandra Nagy, Walter C. Mahoney
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Publication number: 20030165852Abstract: This invention provides methods and compositions useful for identifying and diagnosing rare fetal cells in a mixed cell population such as a maternal blood sample. The methods entail the use of specific nucleic acid probes that hybridize to fetal cell associated RNAs to identify the rare fetal cells or antibodies that bind to polypeptides encoded by the fetal cell associated RNAs for fetal cell detection. The cells detected by the methods of the present invention are useful for diagnosing the fetal cells for a genetic trait of interest, such as trisomy 21. Novel methods for simultaneous screening for fetal cells and diagnosing the fetal cells are also provided. Compositions comprising the fetal cell associated nucleic acids of the invention and their encoded proteins are also provided. The present invention further provides kits useful for practicing the present methods.Type: ApplicationFiled: November 15, 2001Publication date: September 4, 2003Inventors: Paula A. Schueler, Hongxia Xu, Lisa Foltz, Xingyong Wu, Yehsiung Sha, Alexandra Nagy, Walter C. Mahoney