Abstract: A primer for isothermal amplification of nucleic acid and its application are described. The primer includes a forward primer and a reverse primer used for amplifying a target gene. The forward primer is a sequence with an exogenous self-paired hairpin structure at the 5? end, or a sequence with a hairpin structure matched with any source sequence in the target gene close to the forward primer end at the 5? end. Nucleic acid amplification technology using the primer of the invention can produce repeated tandem structure of the target gene, and the repetition times can be controlled precisely. The technology can also be applied to the third generation sequencing technology. The amplification steps are not only simple and fast, but also improve sequencing accuracy.

Abstract: Provided is a nucleic acid isothermal self-amplification method comprising, adding suitable palindrome complementary sequences at both ends of a target template to form a stem-loop structure spontaneously, and providing reagents and conditions as needed to perform self-amplification. The method does not require addition of additional amplification primers. The reagent comprises a DNA polymerase having a strand displacement activity. The method does not rely on exogenous amplification primers for amplification, has a constant amplification temperature without a complex temperature control equipment, and achieves rapid amplification. The amplification product is a long single-stranded DNA of a continuous complementary sequence and can be applied to special occasions. In addition, the amplification has no GC bias.

Abstract: A primer for isothermal amplification of nucleic acid and its application are described. The primer includes a forward primer and a reverse primer used for amplifying a target gene. The forward primer is a sequence with an exogenous self-paired hairpin structure at the 5? end, or a sequence with a hairpin structure matched with any source sequence in the target gene close to the forward primer end at the 5? end. Nucleic acid amplification technology using the primer of the invention can produce repeated tandem structure of the target gene, and the repetition times can be controlled precisely. The technology can also be applied to the third generation sequencing technology. The amplification steps are not only simple and fast, but also improve sequencing accuracy.

Abstract: Provided is a nucleic acid isothermal self-amplification method comprising, adding suitable palindrome complementary sequences at both ends of a target template to form a stem-loop structure spontaneously, and providing reagents and conditions as needed to perform self-amplification. The method does not require addition of additional amplification primers. The reagent comprises a DNA polymerase having a strand displacement activity. The method does not rely on exogenous amplification primers for amplification, has a constant amplification temperature without a complex temperature control equipment, and achieves rapid amplification. The amplification product is a long single-stranded DNA of a continuous complementary sequence and can be applied to special occasions. In addition, the amplification has no GC bias.

Abstract: A preparation method for a high-purity benzoxazoleoxazine ketone compound, a crystal form thereof, and an intermediate compound for preparing a compound of formula (I), and a preparation method therefor.

Abstract: A preparation method for a high-purity benzoxazoleoxazine ketone compound, a crystal form thereof, and an intermediate compound for preparing a compound of formula (I), and a preparation method therefor.