Abstract: The disclosure provides materials and methods for detection of pathogens. In particular, the disclosure provides primers and compositions for detection of viral pathogens, such as SARS-CoV-2. In addition, the disclosure provides a warm-start digital CRISPR assay for detection of viral pathogens, including SARS-CoV-2.

Abstract: A method includes receiving an input specifying a recognition target. The method further includes selecting a plurality of models of an initial recognition layer based on the recognition target, and selecting a plurality of models of a final recognition layer based on the recognition target. The method includes obtaining sensor data from two or more sensors of a plurality of sensors, providing the sensor data to the plurality of models of the initial recognition layer to obtain an initial set of identifications, providing sensor data to the plurality of models of the final recognition layer to obtain a final set of identifications, and outputting an identification from at least one of the initial set of identifications or the final set of identifications.

Abstract: Disclosed herein are “dual-priming” isothermal amplification method (including “self-priming” and “pairing-priming” strand extension, termed “DAMP”) for rapid nucleic acid detection.