Abstract: A test system for stability of video interfaces includes a display, a signal generator, a controller, and an image capturing sensor. The signal generator is configured to transmit multiple video signals to multiple video interfaces of the display. The controller is configured to control the display to sequentially display images according to each video signal. The image capturing sensor is configured to capture the images displayed sequentially by the display according to each video signal, and detect whether an image corresponding to each video signal is abnormal, and transmit a detection result to the controller.

Abstract: An emulsification device disclosed herein comprises: an outer tank having a first pressing end and a first exit end; and an inner tank having a second pressing end and a second exit end, wherein the inner tank is disposed inside the outer tank and the second exit end is located closer than the second pressing end to the first exit end, the outer tank is configured to house a first liquid and the inner tank is configured to house a second liquid, the first and second pressing ends are arranged so that one pressure can be applied onto both the first and second liquids, and under the pressure, the second liquid flows out of the inner tank through the second exit end and contacts with the first liquid in the outer tank so that an emulsion droplet comprising the second liquid within the first liquid is formed.

Abstract: The present invention discloses a pressurized test device and method for in-situ mining natural gas hydrates by jets, relating to the field of exploitation of marine natural gas hydrates. The device comprises an injection system, a jet breakup system, an annular pressure system, an axial pressure system, a backpressure system, a vacuum system, a simulation system, a collecting and processing system and a metering system, all of which can operate independently by controlling pipe valves on pipelines. The loading of the confining pressure of the device is independent of the loading of the axial pressure, without interference to each other. Meanwhile, the jet breakup process of natural gas hydrate-containing sediments can be observed in real time by a video camera.

Abstract: The present invention discloses a pressurized test device and method for in-situ mining natural gas hydrates by jets, relating to the field of exploitation of marine natural gas hydrates. The device comprises an injection system, a jet breakup system, an annular pressure system, an axial pressure system, a backpressure system, a vacuum system, a simulation system, a collecting and processing system and a metering system, all of which can operate independently by controlling pipe valves on pipelines. The loading of the confining pressure of the device is independent of the loading of the axial pressure, without interference to each other. Meanwhile, the jet breakup process of natural gas hydrate-containing sediments can be observed in real time by a video camera.

Abstract: This disclosure provides methods, kits, and compositions to amplify sample DNA of low quantity by adding an amount of buffer DNA before amplification. The method protects the sample DNA against degradation and PCR bias. The method disclosed herein are useful to amplify DNA of low amount, such as genomic DNA from a single cell and therefore are especially useful for genetic diagnostic tests and precision medicine.

Abstract: This disclosure provides methods, kits, and compositions to amplify sample DNA of low quantity by adding an amount of buffer DNA before amplification. The method protects the sample DNA against degradation and PCR bias. The method disclosed herein are useful to amplify DNA of low amount, such as genomic DNA from a single cell and therefore are especially useful for genetic diagnostic tests and precision medicine.

Abstract: This disclosure provides a droplet thermal cycling reaction (DTCR) device comprising a helical tubing through which a colloid flows; a pump that drives the flow of the colloid; and one or more temperature control sheets (TCS). The pump is configured to drive the colloid to flow through the helical tubing. Optionally, the pump is connected to either the inlet or the outlet of the helical tubing. The TCS sheets, configured to control temperatures for reactions occurring on the device, can be placed either outside or inside helical tubing, and contain at least two temperature zones so that the colloid flows through the different temperature zones along inside the helical tubing. The DTCR of this disclosure has technical benefits of reducing device complexity, enabling device miniaturization, reducing PCR reaction volume, dropletizing PCR reactions, and reducing the cost of digital PCR.

Abstract: Algorithm-based molecular assays that involve measurement of expression levels of prognostic and/or predictive genes, or co-expressed genes thereof, from a biological sample obtained from a cancer patient, and analysis of the measured expression levels to provide information concerning the likelihood of recurrence of colorectal cancer and/or the likelihood of a beneficial response to chemotherapy for the patient are provided herein. Methods of analysis of gene expression values of prognostic and/or predictive genes, as well as methods of identifying gene expression-tumor region ratios, tumor-associated stromal surface area, and gene cliques, i.e. genes that co-express with a validated biomarker and thus may be substituted for that biomarker in an assay, are also provided.

Abstract: This disclosure provides methods, kits, and compositions to amplify sample DNA of low quantity by adding an amount of buffer DNA before amplification. The method protects the sample DNA against degradation and PCR bias. The method disclosed herein are useful to amplify DNA of low amount, such as genomic DNA from a single cell and therefore are especially useful for genetic diagnostic tests and precision medicine.

Abstract: This invention provides a quantitative method to determine transcriptionally active regions and quantify sequence abundance from large scale sequencing data. The invention also provides a system based on reference sequences to design and implement the method. The system processes large scale sequence data from high throughput sequencing, generates transcriptionally active region sequences as necessary, and quantifies the sequence abundance of the gene or transcriptionally active region. The method and system are useful for many analyses based on RNA expression profiling.

Abstract: Algorithm-based molecular assays that involve measurement of expression levels of prognostic and/or predictive genes, or co-expressed genes thereof, from a biological sample obtained from a cancer patient, and analysis of the measured expression levels to provide information concerning the likelihood of recurrence of colorectal cancer and/or the likelihood of a beneficial response to chemotherapy for the patient are provided herein. Methods of analysis of gene expression values of prognostic and/or predictive genes, as well as methods of identifying gene expression-tumor region ratios, tumor-associated stromal surface area, and gene cliques, i.e. genes that co-express with a validated biomarker and thus may be substituted for that biomarker in an assay, are also provided.

Abstract: This invention provides a quantitative method to determine transcriptionally active regions and quantify sequence abundance from large scale sequencing data. The invention also provides a system based on reference sequences to design and implement the method. The system processes large scale sequence data from high throughput sequencing, generates transcriptionally active region sequences as necessary, and quantifies the sequence abundance of the gene or transcriptionally active region. The method and system are useful for many analyses based on RNA expression profiling.

Abstract: This invention provides a method for the quantitation and quality assurance of QPCR data. In particular, the invention provides a method for extracting data curves from a QPCR assay, estimating the shape and characteristics of an amplification curve and producing a quality score metric. The invention also provides a robust method for calculating a threshold cycle (CT) value and classifying the status of the QPCR results.

Abstract: This invention provides a method for the quantitation and quality assurance of QPCR data. In particular, the invention provides a method for extracting data curves from a QPCR assay, estimating the shape and characteristics of an amplification curve and producing a quality score metric. The invention also provides a robust method for calculating a threshold cycle (CT) value and classifying the status of the QPCR results.

Abstract: A system for measuring data includes a first processor for extracting data curves from an assay. The first processor estimates a Quality Score (QS) based on the shape and variation of an amplification curve. A second processor calculates a threshold cycle (CT) value. The CT value is the fractional cycle number at which the assay signal rises above a threshold. The processor makes a status classification of the amplification curve. A database is provided to store the QS, C, and classification results.

Abstract: The invention concerns an integrated, qRT-PCR-based system for analyzing and reporting RNA expression profiles of biological samples. In particular, the invention concerns a fully optimized and integrated multiplex, multi-analyte method for expression profiling of RNA in biological samples, including fixed, paraffin-embedded tissue samples. The gene expression profiles obtained can be used for the clinical diagnosis, classification and prognosis of various pathological conditions, including cancer.