Abstract: Provided in the present invention are a method and a kit for multiple detection of respiratory virus nucleic acids, and in particular, disclosed are a method, a primer, a probe and a kit for detecting a plurality of influenza A viruses such as H1N1(2019), H3N2, H5N1, H1N1 and H7N9, influenza B viruses such as Yamagata and Victoria, 2019-nCoV OFR1ab and N genes, and human internal standard gene GAPDH on the basis of a real-time fluorescent quantitative PCR technical platform.

Abstract: The present invention relates to the technical field of sample preservation, and more specifically, to a preservation tube for a sampling swab which includes a tube body. A first cavity for storing a sample releasing agent and a second cavity for placing the sampling swab are provided in the tube body, the first cavity is in communication with the second cavity, and a central axis of the second cavity deviates from a central axis of the tube body. When the present invention is in use, the sampling swab is placed in the second cavity, moved up and down and rotated to elute in the second cavity, and nucleic acid samples of the sampling swab are evenly distributed in the sample releasing agent, and can be used for detection after sucking the sample releasing agent in the first cavity.

Abstract: A microfluidic chip-equipped detection cassette includes a housing, provided with a functional chamber, a first flow channel assembly, a second flow channel assembly, and a result display assembly, wherein the first flow channel assembly and the second flow channel assembly are both in communication with the functional chamber, the functional chamber is in communication with the result display assembly, and the result display assembly is configured to display a detection result.

Abstract: Disclosed are a hand-held nucleic acid detection apparatus equipped with microfluidic chip, and a use method thereof. The detection apparatus includes a sample collection tube, a chip connector, and a detection chip. The chip connector is provided with a piercing tube configured to pierce the sample collection tube. The detection chip is connected to the chip connector. The detection chip is provided with a plurality of chambers and a plurality of flow channels. The flow channels are in communication with the piercing tube and the chambers. The reaction reagent and all the reaction processes are integrated on a chip, with no need of solution injection by virtue of an external instrument, solution transfer or the like operations, and the nucleic acid detection may be carried out even in the case of no dedicated experiment sites or special conditions.

Abstract: To the Abstract: Disclosed in the present invention are a stabilizer for a color developing agent and application thereof, an application of a composition in preparation of the stabilizer, and a kit. A stabilizer for a color developing agent is provided in the present invention. The stabilizer includes a reducing substance and a weakly acidic buffer, and the weakly acidic buffer has a pH of 3.8-6.2. The reducing substance includes one or more of sodium sulfite, sodium bisulfite, sodium thiosulfate, or 1-mercaptoglycerol. The color developing agent includes one or two of a phenothiazine color developing agent or a triphenylmethane color developing agent. The color developing agent can be stably preserved by using the stabilizer. A method for stably preserving a color developing agent includes dissolving the color developing agent in the stabilizer.

Abstract: The present invention discloses a swab sample nucleic acid releaser and use thereof. The sample releaser includes 1-20 mol/L of a metal ion chelating agent, 5-80 mmol/L of a buffer at pH 6-9, 5%-40% (v/v) of a polar organic solvent, 0.2%-10% (v/v) of a surfactant, and 0.1-1 mol/L of a nuclease inhibitor. The sample releaser can integrate three steps of sampling and preservation, inactivation, and nucleic acid extraction into one step, and after sampling, the sample can be directly put into a sampling tube equipped with the sample releaser for storage and transportation; and when in use, the sampling tube is turned upside down and mixed well, and after the sample and the sample releaser are fully mixed, it is let stand for a few minutes at room temperature to release the nucleic acid of the sample.

Abstract: Provided are a separator for separating a nucleic acid amplification system, comprising a polyethylene wax, a solid paraffin wax, and a liquid paraffin wax. Also provided is a method for separating a nucleic acid amplification system, comprising using the separator as a separation layer to separate a nucleic acid amplification system within a same container. The separating layer can be broken by means of applying an external force, thereby mixing the nucleic acid amplification system.

Abstract: The present invention provides a novel coronavirus rapid detection kit based on thermal convection PCR and a method for multiple detection of novel coronavirus nucleic acid. The kit comprises a combination of primers and probes for detecting N genes and ORFlab genes of a novel coronavirus genome.

Abstract: Embodiments of the present disclosure, pertaining to the technical field of high-throughput sequencing, relate to a method for high-throughput sequencing based on an internal reference with a known index. The method includes: generating a random DNA sequence, adding a single-ended adapter DNA sequence containing the known index at both ends of the random DNA sequence to obtain an internal reference sequence, and synthesizing a sequencing quality control sequence based on the internal reference sequence; performing, based on the sequencing quality control sequence, high-throughput sequencing on a library of samples to be tested to obtain sequencing data; and performing result analysis on the sequencing data to obtain a sample error distribution rate of the library of samples to be tested, and ending the high-throughput sequencing. According to the present disclosure, index hopping in the process of sequencing may be monitored based on the internal reference.

Abstract: Disclosed is a kit for testing a COVID-19 antigen. The kit includes a test card, a conjugate pad and a reaction pad being arranged on the test card; wherein the conjugate pad is provided with a labeled antibody Ab1 and a labeled antibody Ab3 of the COVID-19 antigen, and a quality control line and a test line are arranged in parallel with an interval on the reaction pad, the test line being provided with a coating antibody Ab2, and the quality control line being provided with a coating antibody Ab4. With the kit, by the AIE immunofluorescence chromatographic technique, the AIE fluorescent microspheres are used as labels, and the test result of the test card is directly determined and read by using a fluorescent flashlight, or the test result is read by using a fluorescent tester. Such kits have the advantages of high sensitivity, strong specificity and simple operation.

Abstract: The present invention provides a novel coronavirus duplex detection kit. Specifically, the present invention discloses a kit and method for multiplex detection of novel coronavirus 2019-nCoV nucleic acid, which can simultaneously detect two nucleic acid targets of the novel coronavirus 2019-nCoV and possess extremely high sensitivity and specificity, and significantly improve the accuracy of virus identification.

Abstract: A real-time fluorescence RT-PCR detection method and a kit for a novel coronavirus 2019-nCoV. Specifically, the present invention relates to a kit and a method for detecting the nucleic acid of an E gene of the novel coronavirus 2019-nCoV. The kit and the method have extremely high sensitivity and specificity, and can significantly improve the accuracy of virus identification.

Abstract: The present disclosure provides a liquid-based cell preservation solution and a method for preparing the same. The preservation solution includes, by mass percentage: 15 to 20% of an alcohol, 0.4 to 0.7% of a buffer component, 0.2 to 0.5% of an ionic strength modifying agent, 0.05 to 2% of an anticoagulant, 0.05 to 0.2% of a mucus dissociating agent, 0.5 to 0.9% of a preservative, and purified water as the balance. The components of cell preservation solution are set reasonably, and the cells may be preserved at normal temperature for a long time to maintain the cell morphology. Addition of the mucus dissociating agent may effectively soften cell mucus, and separates cells adhered by mucus in the sample, thereby preventing cell accumulation. Addition of the preservative may effectively inhibit the proliferation of mold, bacteria or other pathogens and facilitate cell preservation.

Abstract: The embodiments of the present application, belonging to the technical field of medical test and assay, and provide a preservative for an in vitro diagnostic reagent. The preservative includes a combination of a sulfadoxine solution and a dimethoprim solution, wherein a molar ratio of sulfadoxine in the sulfadoxine solution to dimethoprim in the dimethoprim solution is from 0.002 to 1. The present disclosure also provides use of the preservative for the in vitro diagnostic reagent. The technical solutions according to the embodiments of the present disclosure improve stability of the reagent, do not affect reactions of the reagent, and may be extensively applied.

Abstract: Provided is a nucleic acid detection kit for novel coronavirus 2019-nCoV, and in particular, provided are a kit and a method for multiple detection of the nucleic acid of the novel coronavirus 2019-nCoV. Three nucleic acid targets of the novel coronavirus 2019-nCoV can be detected at the same time. False positives can be prevented by means of mutual authentication among different targets, and detection omissions, which may be caused by mutations, are confirmed, such that the accuracy of virus identification is significantly improved.

Abstract: Disclosed is a method for constructing a second-generation sequencing library of RNA and DNA. The method includes: performing first-strand synthesis on an RNA nucleic acid and a DNA nucleic acid to obtain a first-strand cDNA; performing second-strand synthesis on the first-strand cDNA to obtain a second-strand cDNA; obtaining an A-tailed product by fragmentation, end repair, phosphorylation, and A-tailing on the second-strand cDNA; performing adapter ligation and a first purification treatment on the A-tailed product to obtain a target RNA fragment and DNA fragment, and recovering the same; and carrying out a PCR amplification reaction on target RNA fragment and DNA fragment to construct and obtain the second-generation sequencing library of RNA and DNA.

Abstract: Disclosed is a preparation method for a DNA next-generation sequencing library, including steps: digestion, end repair, and A-tailing of genomic DNA; adapter ligation of DNA fragments; bead purification of product after adapter ligation; PCR amplification of DNA fragments; and selection and purification of PCR product fragments. The preparation method for the DNA next-generation sequencing library includes steps: fractionating by VVN and T7 through a single-step reaction process with double digestion and end repair, blunting a 5?-overhang under the polymerization action of a Taq DNA polymerase, adding an A (adenine) to a 3?-end, and achieving the preparation of the DNA next-generation sequencing library under an integrated single-step reaction. After the single-step reaction ends, bead purification isn't required, so that the preparation process is simple. In the preparation process, there is no preference for two restriction enzymes, which achieves the sequencing of target fragments.

Abstract: The present invention provides an oligonucleotide conjugate with high hybridization performance and use thereof. The oligonucleotide conjugate of the present invention can be used as a probe for nucleic acid detection, the oligonucleotide conjugate of the present invention can be used to design probes more flexibly, and the conservative regions are more accessible. The specificity of the probe is better, and the fluorescence value of the probe can be significantly improved, the fluorescence background is significantly reduced, and the sensitivity of the probe is greatly improved.

Abstract: The present invention provides a heat-resistant DNA polymerase mutant with high amplification activity. Particularly, the present invention uses protein directed evolution technology to construct a random mutation library for the polymerase active domain of Taq enzyme, and gradually adds screening pressure, so that unsuitable mutations will be eliminated naturally, and mutations with dominant traits will gradually accumulate. Finally, a series of amino acid sites and their mutations that are critical to Taq enzyme amplification and polymerization performance will be selected, and a Taq enzyme mutant with high amplification activity will be obtained.

Abstract: The present invention discloses a swab sample nucleic acid releaser and use thereof. The sample releaser includes 1-20 mol/L of a metal ion chelating agent, 5-80 mmol/L of a buffer at pH 6-9, 5%-40% (v/v) of a polar organic solvent, 0.2%-10% (v/v) of a surfactant, and 0.1-1 mol/L of a nuclease inhibitor. The sample releaser can integrate three steps of sampling and preservation, inactivation, and nucleic acid extraction into one step, and after sampling, the sample can be directly put into a sampling tube equipped with the sample releaser for storage and transportation; and when in use, the sampling tube is turned upside down and mixed well, and after the sample and the sample releaser are fully mixed, it is let stand for a few minutes at room temperature to release the nucleic acid of the sample.