Patents by Inventor Xudong Lou
Xudong Lou has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230236105Abstract: Described herein are reagents and methods for improving signal in imaging mass cytometry. Aspects include mass tags with a large number of labeling atoms, chemical modifications to mass tags and additional reagents to reduce background and/or maintain target binding of mass tagged specific binding partners (SBPs), and schemes for associating a plurality of mass tags with a single SBP. As such, embodiments include any combination of one or more reagents and their use. The reagents, kits and methods herein may be used for mass cytometry, including imaging mass cytometry. In some aspects, reagents, kits or methods may be used for delivery of a large number of radioisotopes to a target analyte, for example for therapeutic use or radiometric detection. In certain aspects, only non-radioactive isotopes may be used for mass cytometry.Type: ApplicationFiled: December 18, 2020Publication date: July 27, 2023Applicant: Standard BioTools Canada Inc.Inventors: Bedilu ALLO, Xudong LOU, Peng LIU, Daniel MAJONIS, Anastasia MAVROPOULOS, Ladan GHEIRATMAND
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Publication number: 20210181186Abstract: The present disclosure relates to reagents and their use for elemental imaging mass spectrometry of biological samples.Type: ApplicationFiled: June 8, 2018Publication date: June 17, 2021Applicant: Fluidigm Canada Inc.Inventors: Olga Ornatsky, Qing Chang, Bedilu Allo, Xudong Lou, Alexander Bouzekri
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Publication number: 20200362062Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: ApplicationFiled: May 5, 2020Publication date: November 19, 2020Inventors: MITCHELL A. WINNIK, MARK NITZ, VLADIMIR BARANOV, XUDONG LOU
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Patent number: 10752707Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: GrantFiled: July 9, 2019Date of Patent: August 25, 2020Assignee: FLUIDIGM CANADA INC.Inventors: Mitchell A. Winnik, Mark Nitz, Vladimir Baranov, Xudong Lou
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Patent number: 10669358Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: GrantFiled: July 27, 2018Date of Patent: June 2, 2020Assignee: FLUIDIGM CANADA INC.Inventors: Mitchell A. Winnik, Mark Nitz, Vladimir Baranov, Xudong Lou
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Publication number: 20190345272Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: ApplicationFiled: July 9, 2019Publication date: November 14, 2019Inventors: MITCHELL A. WINNIK, MARK NITZ, VLADIMIR BARANOV, XUDONG LOU
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Publication number: 20190077890Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: ApplicationFiled: July 27, 2018Publication date: March 14, 2019Applicant: FLUIDIGM CANADA INC.Inventors: MITCHELL A. WINNIK, MARK NITZ, VLADIMIR BARANOV, XUDONG LOU
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Patent number: 10072104Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: GrantFiled: October 5, 2015Date of Patent: September 11, 2018Assignee: Fluidigm Canada Inc.Inventors: Mitchell A. Winnik, Mark Nitz, Vladimir Baranov, Xudong Lou
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Patent number: 9296838Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: GrantFiled: March 16, 2015Date of Patent: March 29, 2016Assignee: Fluidigm Canada, Inc.Inventors: Mitchell A. Winnik, Mark Nitz, Vladimir Baranov, Xudong Lou
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Publication number: 20160025731Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: ApplicationFiled: October 5, 2015Publication date: January 28, 2016Inventors: MITCHELL A. WINNIK, MARK NITZ, VLADIMIR BARANOV, XUDONG LOU
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Patent number: 9169335Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: GrantFiled: March 16, 2015Date of Patent: October 27, 2015Assignee: Fluidigm Canada, Inc.Inventors: Mitchell A. Winnik, Mark Nitz, Vladimir Baranov, Xudong Lou
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Publication number: 20150183895Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: ApplicationFiled: March 16, 2015Publication date: July 2, 2015Inventors: Mitchell A. Winnik, MARK NITZ, VLADIMIR BARANOV, XUDONG LOU
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Patent number: 9012239Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: GrantFiled: May 28, 2007Date of Patent: April 21, 2015Assignee: Fluidigm Canada Inc.Inventors: Mitchell A. Winnik, Mark Nitz, Vladimir Baranov, Xudong Lou
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Publication number: 20080003616Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: ApplicationFiled: May 28, 2007Publication date: January 3, 2008Inventors: Mitchell Winnik, Mark Nitz, Vladimir Baranov, Xudong Lou
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Publication number: 20040144655Abstract: Process for depositing by electrografting a strong adherent polymer coating onto an electrically conductive surface comprising the step of electrochemical grafting of an active monomer for forming a primer coating P onto the surface and having as general formula: X0 (meth)acrylate wherein X is either part of a preformed polymer or is an intermediate agent for polyaddition reaction or is an anchoring group for attachment of a molecule having at least one complementary reactive group. Such process allows formation of new primer by one-step electro-grafting of a macromonomer. Such process also allows further modification of an initial electrografted polymer to increase the coating thickness by the grafting-from technique. Such process also allows to graft onto the primer coating compounds like functional polymer, peptide, protein, oligonucleotide, dyes, drugs, anti-bacterian compounds.Type: ApplicationFiled: December 4, 2003Publication date: July 29, 2004Inventors: Olivier Bertrand, Robert Jerome, Sandrine Gautier, Veronique Maquet, Christophe Detrembleur, Christine Jerome, Samuel Voccia, Michael Claes, Xudong Lou, David-Emmanuel Labaye