Abstract: Disclosed are a microfluidic system and method for isolating target cells or vesicles in a fluid. The system of the present invention comprises a fluid passageway having an inlet and an outlet; one or more ultra-high frequency acoustic resonator capable of generating bulk acoustic waves in the fluid passageway at a frequency of about 0.5-50 GHz; a power regulator which adjusts the power of the bulk acoustic waves generated by the ultra-high frequency resonator; and a flow rate regulating device that regulates the velocity of the solution flowing through the bulk acoustic wave region. Adjusting the power of the generated bulk acoustic waves by means of the power regulator and/or adjusting the velocity of the solution flowing through the bulk acoustic wave region by means of the flow rate regulating device allow cells or vesicles to stay in a bulk acoustic wave-affected region.

Abstract: A microfluidic control system and method for separating flexible particles such as cell vesicles or biomacromolecules such as exosomes in a sample. The system of the present invention comprises one or more ultrahigh frequency acoustic resonators. The ultrahigh frequency acoustic resonators are capable of generating in a fluid channel an acoustic wave of which the frequency is about 0.5-50 GHz and propagated towards a wall opposite the fluid channel. By adjusting the power of the generated acoustic wave and/or the speed at which a conditioning solution flows through an acoustic wave area, flexible particles in a specified range are pushed to and remain at the top part of the flow channel in the acoustic wave area, while flexible particles outside of the specified range go downstream via the acoustic wave area to be collected, thus capturing or releasing the flexible particles in a solution such as cell vesicles or biomacromolecules, particularly exosomes.

Abstract: A microfluidic control system and method for controlling the movement of target particles in a fluid. The microfluidic system comprises a fluid channel which is provided with an inlet and a plurality of outlets, and one or more ultra-high frequency sound wave resonators which may generate a bulk acoustic wave having a frequency of about 0.5-50 GHz in the fluid channel; by means of adjusting the shape and orientation of a bulk acoustic wave generating region of the ultra-high frequency bulk acoustic wave resonator, the particles enter a vortex channel caused by the bulk acoustic wave in a solution and move according to the specified position and direction, and the particles in the solution may be controlled and separated to obtain specified particles, or obtain a purified solution after the particles are separated.

Abstract: A PETx polymer including a main backbone which is a first poly-L-lysine, and a side chain which is sequentially connected with a first polyethylene glycol and a second poly-L-lysine, where the second poly-L-lysine is saturately connected with a second polyethylene glycol and a third polyethylene glycol with no remaining amino groups, and the third polyethylene glycol is connected with a functional group at its end, where the first poly-L-lysine and the second poly-L-lysine have the same or different chain lengths, and the first polyethylene glycol, the second polyethylene glycol and the third polyethylene glycol have the same or different chain lengths. Preferably, the PETx polymer is PLL-g-{PEGk-PLL-g-[(PEGj-biological recognition group)y %(PEGi)1-y %]}x %, where i, j, k, m, and n are all integers greater than or equal to 1, j is not equal to i, and x and y are all greater than 0 and less than 100.

Abstract: A PETx polymer including a main backbone which is a first poly-L-lysine, and a side chain which is sequentially connected with a first polyethylene glycol and a second poly-L-lysine, where the second poly-L-lysine is saturately connected with a second polyethylene glycol and a third polyethylene glycol with no remaining amino groups, and the third polyethylene glycol is connected with a functional group at its end, where the first poly-L-lysine and the second poly-L-lysine have the same or different chain lengths, and the first polyethylene glycol, the second polyethylene glycol and the third polyethylene glycol have the same or different chain lengths. Preferably, the PETx polymer is PLL-g-{PEGk-PLL-g-[(PEGj-biological recognition group)y%(PEGi)1-y%]}x%, where i, j, k, m, and n are all integers greater than or equal to 1, j is not equal to i, and x and y are all greater than 0 and less than 100.

Abstract: The present invention provides a regenerative nanosensor device for the detection of one or more analytes of interest. In certain embodiments, the device comprises a nanostructure having a reversible functionalized coating comprising a supramolecular assembly. Controllable and selective disruption of the assembly promotes desorption of at least part of the reversible functionalized coating thereby allowing for reuse of the regenerative device.

Abstract: The present invention provides a regenerative nanosensor device for the detection of one or more analytes of interest. In certain embodiments, the device comprises a nanostructure having a reversible functionalized coating comprising a supramolecular assembly. Controllable and selective disruption of the assembly promotes desorption of at least part of the reversible functionalized coating thereby allowing for reuse of the regenerative device.