Patents by Inventor Yanghua XU

Yanghua XU has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methionine lyase, encoding gene and biosynthetic method thereof
    Patent number: 10308925
    Abstract: Embodiment of the present invention discloses a kind of methionine lyase and its encoding gene and biosynthetic method. According to the present invention, the gene encoding methionine lyase as shown in SEQ ID No. 1 is separated from the genome of C. rosea. Embodiment of the present invention further provides an efficient biosynthetic method of methionine lyase, comprising: (1) cloning gene (shown in SEQ ID No.1) encoding methionine degradation enzyme into a yeast expression vector to construct recombinant yeast expression vector; (2) transforming the recombinant yeast expression vector into Saccharomyces cerevisiae to obtain expression strain; (3) inducing the expression strain to express the methionine lyase, collecting induced strains, purifying expressed recombinant methionine lyase. Purity of recombinant methionine lyase prepared according to the present invention is above 90%, and its efficiency of degradating methionine can reach 0.53±0.0030 ?M MTL·h?1·mg protein?1.
    Type: Grant
    Filed: December 3, 2015
    Date of Patent: June 4, 2019
    Assignee: HUBEI UNIVERSITY OF TECHNOLOGY
    Inventors: Yajie Tang, Kaizhi Jia, Yanghua Xu, Quan Zhang, Hongmei Li
  • METHIONINE LYASE, ENCODING GENE AND BIOSYNTHETIC METHOD THEREOF
    Publication number: 20170260518
    Abstract: Embodiment of the present invention discloses a kind of methionine lyase and its encoding gene and biosynthetic method. According to the present invention, the gene encoding methionine lyase as shown in SEQ ID No. 1 is separated from the genome of C. rosea. Embodiment of the present invention further provides an efficient biosynthetic method of methionine lyase, comprising: (1) cloning gene (shown in SEQ ID No. 1) encoding methionine degradation enzyme into a yeast expression vector to construct recombinant yeast expression vector; (2) transforming the recombinant yeast expression vector into Saccharomyces cerevisiae to obtain expression strain; (3) inducing the expression strain to express the methionine lyase, collecting induced strains, purifying expressed recombinant methionine lyase. Purity of recombinant methionine lyase prepared according to the present invention is above 90%, and its efficiency of degradating methionine can reach 0.53±0.0030 ?M MTL·h?1·mg protein?1.
    Type: Application
    Filed: December 3, 2015
    Publication date: September 14, 2017
    Applicant: Hubei University Of Technology
    Inventors: Yajie TANG, Kaizhi JIA, Yanghua XU, Quan ZHANG, Hongmei LI