Abstract: A method for producing benzaldehyde is provided. Benzaldehyde is produced by a method including a step of using amino acid deaminase (AAD), 4-hydroxymandelate synthase (HMAS), (S)-mandelate dehydrogenase (SMDH), and benzoylformate decarboxylase (BFDC) over a time period, wherein the producing step is carried out in the presence of catalase during a portion of said time period.

Abstract: A method for producing benzaldehyde is provided. Benzaldehyde is produced from L-phenylalanine or a carbon source by using microorganism(s) having amino acid deaminase, 4-hydroxymandelate synthase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase.

Abstract: A method for producing benzaldehyde is provided. Benzaldehyde is produced from L-phenylalanine or a carbon source by using microorganism(s) having amino acid deaminase, 4-hydroxymandelate synthase, (S)-mandelate dehydrogenase, and benzoylformate decarboxylase.

Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.

Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.

Abstract: It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.

Abstract: It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.

Abstract: The present invention provides a method of producing 2R,4R-Monatin with a good yield using inexpensive L-Trp rather than expensive D-Trp as a stating material. Specifically, the present invention provides a method for producing 2R,4R-Monatin or a salt thereof, comprising: (1) contacting L-tryptophan with a deamination enzyme to form indole-3-pyruvate; (2) contacting the indole-3-pyruvate and pyruvate with an aldolase to form 4R-IHOG; and (3) contacting the 4R-IHOG with a D-aminotransferase in the presence of a D-amino acid to form the 2R,4R-Monatin; and the like. In (3), it is preferable to use a D-aminotransferase having no or low ability to form D-tryptophan from indole-3-pyruvate.

Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.

Abstract: [Problem] Providing a methodology for improving a yield of 2R,4R-Monatin. [Means for solving the Problem] A method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and oxidizing a tryptophan to form the indole-3-pyruvate.

Abstract: The present invention provides a method for efficiently producing a ?-alanyl-amino acid (e.g., carnosine) or derivative thereof. Specifically, it provides a method for producing a ?-alanyl-amino acid (e.g., ?-alanyl-histidine) or derivative thereof, by reacting a ?-alanyl ester or a ?-alanyl amide and an amino acid or derivative thereof in the presence of an enzyme or an enzyme-containing product that has an ability to form the ?-alanyl-amino acid (e.g., ?-alanyl-histidine) or derivative thereof from the ?-alanyl ester or the ?-alanyl amide and the amino acid (e.g., histidine) or derivative thereof. The present invention also provides a protein which is able to catalyze production of a ?-alanyl-amino acid or derivative thereof from a ?-alanyl ester or a ?-alanyl amide and an amino acid or derivative thereof, and a polynucleotide encoding said protein.

Abstract: It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.

Abstract: The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and an aldolase which can be used in these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).