Abstract: A recombinant protein and a preparation method and application thereof are provided. The recombinant protein includes a peptide, and the peptide includes an amino acid sequence after substitution of the sequence of SEQ ID NO: 1. The substitution includes at least one selected from the group consisting of the following substitutions: the amino acid at position 182 of SEQ ID NO: 1 is substituted with aspartic acid (Asp); the amino acid at position 268 of SEQ ID NO: 1 is substituted with aspartic acid (Asp); and the amino acid at position 384 of SEQ ID NO: 1 is substituted with tyrosine (Tyr). The recombinant protein is suitable for detecting glycated hemoglobin.

Abstract: The fructosyl amino acid oxidase (FPOX-CE) of other species, wild-type fructosyl amino acid oxidase (PnFPOX), and the recombinant proteins (PnFPOX-SS, PnFPOX-DS, and PnFPOX-TS) were respectively dissolved in a 100 mM potassium phosphate buffer (pH value of 8.0) to achieve a concentration of 0.05 mg/ml, and after a heat treatment at 25° C. to 60° C. for 10 minutes, the oxidase activities of the above-mentioned fructosyl amino acid oxidases and recombinant proteins were measured. At the same time, thermal stability (%) was calculated based on the following formula: thermal stability=(value of oxidase activity after heat treatment)/(value of oxidase activity without heat treatment)×100%.

Abstract: Apparatus for immunoassay includes: a cartridge, including at least one test unit; a pin-film assembly, having a second sealing film, a plurality of pierce mechanisms, and a first actuation unit; a plurality of magnetic particles; at least one first magnetic unit; and at least one second magnetic unit. The test unit includes a plurality of fluid chambers, a plurality of pin chambers, a microchannel structure, a buffer chamber, a detection chamber and a waste chamber. The first actuation unit drives the pierce mechanisms to enable a working fluid to flow into the detection chamber storing the magnetic particles. As the second magnetic unit has a magnetic force larger than that of the first magnetic unit and can move reciprocatingly between a third position and a fourth position, the magnetic particles are driven to move reciprocatingly inside the detection chamber, thereby fully mixing the magnetic particles with the working fluid.

Abstract: The present invention provides a biocompatible bilayer porous matrix and preparation thereof. The bilayer porous matrix is composed of gelatin, chondroitin 6 sulfate, and hyaluronic acid, also, prepared through freeze-drying technique at different temperature and time duration to form varied pore sizes on each layer. The present invention also provides a method of cell culture using the bilayer porous matrix.