Abstract: Provided herein are polynucleotides comprising novel bidirectional dual expression cassettes, recombination adeno-associated virus (rAAV) comprising these polynucleotides, and methods of making and using the polynucleotides and rAAV. Also provided are novel transcriptional control elements (e.g., promoters, enhancers, introns, polyadenylation sequences, and combinations thereof), and novel antibody coding sequences. The compositions and methods disclosed herein are particularly advantageous in that they allow for the efficient expression of two different polypeptides (e.g., an antibody heavy chain and an antibody light chain) in a cell. In particular, they allow for the efficient expression of antibodies (e.g., anti-C5 antibodies) in a subject, for the treatment of diseases (e.g., C5-mediated diseases, such as PNH).

Abstract: Provided are recombinant adeno-associated virus (rAAV) compositions for the expression of antibodies (e.g., anti-complement component 5 (C5) antibodies) in cells, and methods of treating disorders with the same (e.g., disorders associated with C5 activity (e.g., Paroxysmal Nocturnal Hemoglobinuria)). Also provided are compositions, systems and methods for making the rAAV compositions.

Abstract: Pressure and flow measurements made during extraction of fluid samples from a subsurface earth formation using a borehole logging tool having a single extraction probe are analyzed to derive separate values for both horizontal and vertical formation permeability. Build-up measurements are used to derive the slope of variation of formation pressure with respect to a spherical time function, and this value is incorporated in an expression for a dimensionless variable relating pressure, flowrate, porosity, compressibility and probe radius. The resulting value of the dimensionless constant provides an index into a look-up table obtained by a new analysis of the fluid dynamics in the immediate vicinity of the probe for an anisotropic formation. The table gives values for two or more dimensionless variables from which the permeability values are derived.