Patents by Inventor Yohei SASAGAWA

Yohei SASAGAWA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHOD FOR NUCLEIC ACID AMPLIFICATION
    Publication number: 20200048693
    Abstract: A kit for amplifying a nucleic acid, using RNA as a template, which can realize elimination of the risk of non-specific amplification caused by DNA mixed from reagents and/or working environment, an increase in the detection sensitivity of trace RNA, and a reduction in amplification bias. The kit includes a degrading enzyme specific to DNA in an RNA-DNA hybrid that is a double strand-specific DNA degrading enzyme or a non-specific DNA degrading enzyme, and an RNase H minus reverse transcriptase. If the degrading enzyme specific to DNA in an RNA-DNA hybrid is a non-specific DNA degrading enzyme, then the kit further comprises a single-stranded DNA-binding protein.
    Type: Application
    Filed: September 12, 2019
    Publication date: February 13, 2020
    Applicant: RIKEN
    Inventors: Tetsutaro HAYASHI, Yohei SASAGAWA, Itoshi NIKAIDO
  • Method for nucleic acid amplification
    Patent number: 10457982
    Abstract: It is an object of the present invention to provide a method for amplifying a nucleic acid, using RNA as a template, which can realize elimination of the risk of non-specific amplification caused by DNA mixed from reagents and/or working environment, an increase in the detection sensitivity of trace RNA, and a reduction in amplification bias. According to the present invention, there is provided a method for amplifying a nucleic acid, which comprises a step of incubating a mixture comprising template RNA, a primer, a degrading enzyme specific to DNA in RNA-DNA hybrid, an RNase H minus reverse transcriptase, and a substrate.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: October 29, 2019
    Assignee: RIKEN
    Inventors: Tetsutaro Hayashi, Yohei Sasagawa, Itoshi Nikaido
  • METHOD FOR NUCLEIC ACID AMPLIFICATION
    Publication number: 20170275685
    Abstract: It is an object of the present invention to provide a method for amplifying a nucleic acid, using RNA as a template, which can realize elimination of the risk of non-specific amplification caused by DNA mixed from reagents and/or working environment, an increase in the detection sensitivity of trace RNA, and a reduction in amplification bias. According to the present invention, there is provided a method for amplifying a nucleic acid, which comprises a step of incubating a mixture comprising template RNA, a primer, a degrading enzyme specific to DNA in RNA-DNA hybrid, an RNase H minus reverse transcriptase, and a substrate.
    Type: Application
    Filed: September 30, 2015
    Publication date: September 28, 2017
    Applicant: RIKEN
    Inventors: Tetsutaro HAYASHI, Yohei SASAGAWA, Itoshi NIKAIDO