Patents by Inventor Yonathan Zohar

Yonathan Zohar has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10709119
    Abstract: Methods are provided for delivery of at least one agent into egg(s) from an egg-producing aquatic animal including contacting fertilized or unfertilized egg(s) from said egg-producing aquatic animal with the at least one agent in the presence of a guanidine-containing compound capable of enhancing the permeability of the chorion of the egg(s). Methods are provided for the drug screening and compound toxicity assays. Methods are also provided for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of agents that lead to the failure of fertile gonadal development. Agents may be delivered to the eggs directly before the fertilization or post fertilization by contacting eggs in an immersion medium including the agent of interest.
    Type: Grant
    Filed: May 17, 2016
    Date of Patent: July 14, 2020
    Assignee: UNIVERSITY OF MARYLAND BALTIMORE COUNTY
    Inventors: Ten-Tsao Wong, Yonathan Zohar
  • Publication number: 20190104710
    Abstract: Methods for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of compounds that lead to the failure of fertile gonadal development. Compounds may be delivered to the eggs prior to fertilization or water activation or post fertilization and water activation by contacting unfertilized or pre-water-activated fertilized eggs or fertilized eggs in an immersion medium including the compound of interest. Compounds may be conjugated with a molecular transporter compound effective for chorionic transport of the conjugate. The compounds may be antisense Morpholino oligomers that are capable of effectively suppressing the expression of the dead end gene or other essential genes for germ cell development in fish and other egg-producing aquatic animals.
    Type: Application
    Filed: October 22, 2018
    Publication date: April 11, 2019
    Inventors: Yonathan Zohar, Ten-Tsao Wong
  • Patent number: 10136621
    Abstract: Methods for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of compounds that lead to the failure of fertile gonadal development. Compounds may be delivered to the eggs prior to fertilization or water activation or post fertilization and water activation by contacting unfertilized or pre-water-activated fertilized eggs or fertilized eggs in an immersion medium including the compound of interest. Compounds may be conjugated with a molecular transporter compound effective for chorionic transport of the conjugate. The compounds may be antisense Morpholino oligomers that are capable of effectively suppressing the expression of the dead end gene or other essential genes for germ cell development in fish and other egg-producing aquatic animals.
    Type: Grant
    Filed: June 18, 2018
    Date of Patent: November 27, 2018
    Assignee: UNIVERSITY OF MARYLAND BALTIMORE COUNTY
    Inventors: Yonathan Zohar, Ten-Tsao Wong
  • Publication number: 20180295817
    Abstract: Methods are provided for delivery of at least one agent into egg(s) from an egg-producing aquatic animal including contacting fertilized or unfertilized egg(s) from said egg-producing aquatic animal with the at least one agent in the presence of a guanidine-containing compound capable of enhancing the permeability of the chorion of the egg(s). Methods are provided for the drug screening and compound toxicity assays. Methods are also provided for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of agents that lead to the failure of fertile gonadal development. Agents may be delivered to the eggs directly before the fertilization or post fertilization by contacting eggs in an immersion medium including the agent of interest.
    Type: Application
    Filed: May 17, 2016
    Publication date: October 18, 2018
    Applicant: University of Maryland Baltimore County
    Inventors: Ten-Tsao Wong, Yonathan Zohar
  • Publication number: 20180288984
    Abstract: Methods for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of compounds that lead to the failure of fertile gonadal development. Compounds may be delivered to the eggs prior to fertilization or water activation or post fertilization and water activation by contacting unfertilized or pre-water-activated fertilized eggs or fertilized eggs in an immersion medium including the compound of interest. Compounds may be conjugated with a molecular transporter compound effective for chorionic transport of the conjugate. The compounds may be antisense Morpholino oligomers that are capable of effectively suppressing the expression of the dead end gene or other essential genes for germ cell development in fish and other egg-producing aquatic animals.
    Type: Application
    Filed: June 18, 2018
    Publication date: October 11, 2018
    Inventors: Yonathan Zohar, Ten-Tsao Wong
  • Patent number: 9999208
    Abstract: Methods for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of compounds that lead to the failure of fertile gonadal development. Compounds may be delivered to the eggs prior to fertilization or water activation or post fertilization and water activation by contacting unfertilized or pre-water-activated fertilized eggs or fertilized eggs in an immersion medium including the compound of interest. Compounds may be conjugated with a molecular transporter compound effective for chorionic transport of the conjugate. The compounds may be antisense Morpholino oligomers that are capable of effectively suppressing the expression of the dead end gene or other essential genes for germ cell development in fish and other egg-producing aquatic animals.
    Type: Grant
    Filed: November 14, 2014
    Date of Patent: June 19, 2018
    Assignee: UNIVERSITY OF MARYLAND BALTIMORE COUNTY
    Inventors: Yonathan Zohar, Ten-Tsao Wong
  • Publication number: 20160286768
    Abstract: Methods for the production of reproductively sterile fish and aquatic animals for aquaculture, the aquarium trade, and control of invasive species are described. The methods include disruption of gonadal development through the administration of compounds that lead to the failure of fertile gonadal development. Compounds may be delivered to the eggs prior to fertilization or water activation or post fertilization and water activation by contacting unfertilized or pre-water-activated fertilized eggs or fertilized eggs in an immersion medium including the compound of interest. Compounds may be conjugated with a molecular transporter compound effective for chorionic transport of the conjugate. The compounds may be antisense Morpholino oligomers that are cap able of effectively suppressing the expression of the dead end gene or other essential genes for germ cell development in fish and other egg-producing aquatic animals.
    Type: Application
    Filed: November 14, 2014
    Publication date: October 6, 2016
    Applicant: University of Maryland Baltimore County
    Inventors: Yonathan Zohar, Ten-Tsao Wong
  • Patent number: 7194978
    Abstract: The present invention provides for a method for inducing sterility in fish by administering at least one compound that disrupts the establishment of the gonadotropin-releasing hormone system during early development thereby inhibiting sexual maturity in the treated fish. Effective compounds include GABA, GABA receptor agonists, or GABA receptor antagonists.
    Type: Grant
    Filed: August 23, 2004
    Date of Patent: March 27, 2007
    Assignees: University of Maryland Biotechnology Institute, Ramot at Tel Aviv University, United States of America
    Inventors: Yonathan Zohar, Yoav Gothilf, Susan Wray
  • Patent number: 7082893
    Abstract: The present invention relates to a two-stage biofilter reactor system for removing nitrogenous compounds from a recirculating aquaculture system. The system includes a aerobic nitrification unit and a downstream anaerobic denitrification unit, wherein both units have a mobile bed of suspended media whereon bacteria can grow and reduce nitrate and/or ammonia levels in the recirculating aquaculture system. Use of the two-stage system has the advantage of reducing the water exchange rates and consumption of salt when maintaining a saline effluent.
    Type: Grant
    Filed: April 5, 2004
    Date of Patent: August 1, 2006
    Assignee: University of Maryland Biotechnology Institute
    Inventors: Harold J. Schreier, Yossi Tal, Yonathan Zohar
  • Publication number: 20050132969
    Abstract: The present invention provides for a method for inducing sterility in fish by administering at least one compound that disrupts the establishment of the gonadotropin-releasing hormone system during early development thereby inhibiting sexual maturity in the treated fish. Effective compounds include GABA, GABA receptor agonists, or GABA receptor antagonists.
    Type: Application
    Filed: August 23, 2004
    Publication date: June 23, 2005
    Inventors: Yonathan Zohar, Yoav Gothilf, Susan Wray
  • Publication number: 20040244715
    Abstract: The present invention relates to a two-stage biofilter reactor system for removing nitrogenous compounds from a recirculating aquaculture system. The system includes a aerobic nitrification unit and a downstream anaerobic denitrification unit, wherein both units have a mobile bed of suspended media whereon bacteria can grow and reduce nitrate and/or ammonia levels in the recirculating aquaculture system. Use of the two-stage system has the advantage of reducing the water exchange rates and consumption of salt when maintaining a saline effluent.
    Type: Application
    Filed: April 5, 2004
    Publication date: December 9, 2004
    Inventors: Harold J. Schreier, Yossi Tal, Yonathan Zohar
  • Patent number: 6584935
    Abstract: The present invention relates to a recirculating marine aquaculture process for production of a crab species, including (i) a broodstock conditioning, (ii) spawning, (iii) egg incubation, (iv) larval growth, (v) nursery post-larval growth, and (vi) grow-out of the crab to a final product weight, in which each stage (i)-(vi) of the process involves operation in an aqueous medium that is coupled in liquid recirculation relationship with waste component removal from the aqueous medium and returning purified aqueous medium to the external environment. The process involves operation in a closed, recirculating aquaculture system in which photoperiod, water temperature, water chemistry, and diet are optimized and then continuously monitored and controlled to obtain optimal production at each of the six phases (i)-(vi) of the life cycle.
    Type: Grant
    Filed: August 19, 2002
    Date of Patent: July 1, 2003
    Assignee: University of Maryland Biotechnology Institute
    Inventors: Yonathan Zohar, Oded Zmora, Anson Hines
  • Publication number: 20030070624
    Abstract: The present invention relates to a recirculating marine aquaculture process for production of a crab species, including (i) a broodstock conditioning, (ii) spawning, (iii) egg incubation, (iv) larval growth, (v) nursery post-larval growth, and (vi) grow-out of the crab to a final product weight, in which each stage (i)-(vi) of the process involves operation in an aqueous medium that is coupled in liquid recirculation relationship with means for removing waste components from the aqueous medium and returning purified aqueous medium to the external environment. The process involves operation in a closed, recirculating aquaculture system in which photoperiod, water temperature, water chemistry, and diet are optimized and then continuously monitored and controlled to obtain optimal production at each of the six phases (i)-(vi) of the life cycle.
    Type: Application
    Filed: August 19, 2002
    Publication date: April 17, 2003
    Inventors: Yonathan Zohar, Oded Zmora, Anson Hines
  • Patent number: 6443097
    Abstract: A high efficiency recirculating marine aquaculture process for producing fish at variable yield densities of up to 60 kg/meter3 of the aquaculture tank, including fish that spawn under short photoperiods such as gilthead seabream (Sparus aurata) as well as fish that spawn under long photoperiods such as striped bass (Morone saxatilis). The process involves broodstock conditioning, spawning/reproduction, larval growth, nursery, and adult grow-out operations. The process is characterized by high-rate growth at optimal process conditions requiring less than 10% daily water exchange, thereby enabling effluent waste from the system to be discharged to a municipal sewer after disinfection treatment, which in turn permits siting of the aquaculture process facility in urban/suburban locations where aquaculture processes have been previously infeasible.
    Type: Grant
    Filed: March 16, 2001
    Date of Patent: September 3, 2002
    Assignee: University of Maryland Biotechnology Institute
    Inventors: Yonathan Zohar, Stanley Serfling, John Stubblefield, Alan Place, Mordechai Harrel
  • Patent number: 6210927
    Abstract: Novel peptide hormones which influence the release of gonadotropins by the pituitary gland in fish are disclosed. Methods in which such peptides may be administered to fish to control their reproduction are also described. Furthermore, novel isolated cDNA encoding the precursor of the novel gonadotropin-releasing hormone is disclosed. The use of such cDNA in controlling the gonadal development and spawning of fish is also described.
    Type: Grant
    Filed: June 30, 1997
    Date of Patent: April 3, 2001
    Assignee: University of Maryland Biotechnology Institute
    Inventors: Yonathan Zohar, Yoav Gothilf
  • Patent number: 5643877
    Abstract: Novel peptide hormones which influence the release of gonadotropins by the pituitary gland in fish are disclosed. Methods in which such peptides may be administered to fish to control their reproduction are also described. Furthermore, novel isolated cDNA encoding the precursor of the novel gonadotropin-releasing hormone is disclosed. The use of such cDNA in controlling the gonadal development and spawning of fish is also described.
    Type: Grant
    Filed: December 5, 1994
    Date of Patent: July 1, 1997
    Assignees: University of Maryland Biotechnology Institute, University of Victoria Innovation and Development Corporation
    Inventors: Yonathan Zohar, Nancy M. Sherwood, Jean Rivier, Jim Powell, Yoav Gothilf
  • Patent number: 5076208
    Abstract: A method for administering compounds, including proteins, non-protein drugs, and nucleic acids, to aquatic animals, especially fish, in an aquatic medium wherein the compound is added to the medium and ultrasound is applied to the medium to enhance or effect the uptake of the compound by the animal. In one example, gonadotropin-releasing hormone analogue (GnRHa) was administered to fish via water to which ultrasound was applied for ten to fifteen minutes at an intensity of 1.7 W/cm.sup.2. Fish treated with ultrasound had blood levels of 3.29.+-.1.0 ng/ml of GnRHa as compared to levels of 0.50.+-.0.23 ng/ml for fish exposed to GnRHa in the absence of ultrasound.
    Type: Grant
    Filed: September 14, 1990
    Date of Patent: December 31, 1991
    Assignee: Massachusetts Institute of Technology
    Inventors: Yonathan Zohar, Antony D'Emanuele, Joesph Kost, Robert S. Langer