Abstract: Provided is use of immobilized transaminase in preparation of sitagliptin and/or (R)-3-amino-1-morpholine-4-(2,4,5-trifluorophenyl)-1-butanone. The immobilized transaminase comprises resin and a transaminase mutant, the amino acid sequence of the transaminase mutant is as shown in SEQ ID NO: 3 or SEQ ID NO: 7. Also provided is an immobilized transaminase, a transaminase mutant, a preparation method therefor and use thereof. The enzyme activity of the transaminase mutant in the catalysis of a ketoamide substrate is high, and the enzyme activity is still high after the transaminase mutant is prepared into the immobilized transaminase.

Abstract: A charging method includes that after receiving a charging request, a master control device arranges a parking space for an electric vehicle to be charged and sends a traction device a movement instruction generated from the charging request; the traction device drives, according to the movement instruction, a charging controller to move along a guide rail to above the electric vehicle to be charged; the master control device sends a cable transferring instruction to a cable transferring device and the cable transferring device starts to lower a cable; after the cable transferring device lowers the cable to a preset position, the master control device sends a stop instruction to the cable transferring device and the cable transferring device stops lowering the cable; and the cable charges the electric vehicle to be charged.

Abstract: A charging method includes that after receiving a charging request, a master control device arranges a parking space for an electric vehicle to be charged and sends a traction device a movement instruction generated from the charging request; the traction device drives, according to the movement instruction, a charging controller to move along a guide rail to above the electric vehicle to be charged; the master control device sends a cable transferring instruction to a cable transferring device and the cable transferring device starts to lower a cable; after the cable transferring device lowers the cable to a preset position, the master control device sends a stop instruction to the cable transferring device and the cable transferring device stops lowering the cable; and the cable charges the electric vehicle to be charged.

Abstract: A method for charging an electric vehicle includes steps that a master device sorts multiple received charging request information according to a preset sorting rule, where the charging request information includes position information of the electric vehicle to be charged; the master device sends a charging instruction to a charging system according to a sorting result; the charging system receives and processes the charging instruction and then transmits the charging instruction to an automated guided vehicle; and the automated guided vehicle travels, according to the position information, to a position of the electric vehicle to be charged, and charges the electric vehicle to be charged.

Abstract: A grounding grid breakpoint diagnostic method for a transient electromagnetic method, based on a transient electromagnetic detection apparatus. The method comprises: (101) disposing a test line at a position above a grounding grid to be diagnosed on the ground, and determining coordinates of a detection point; (102) obtaining information about coordinates of the detection point, and coinciding the center of the transient electromagnetic detection apparatus with the coordinates of the detection point; (103) performing, by the transient electromagnetic detection apparatus, measurement along the test line point by point, so as to obtain measurement data; (104) processing, by using diagram forming software, the measurement data to form a longitudinal resistivity cross-section diagram of the detection point according to a preset formula; (105) determining whether a breakpoint occurs at the detection point according to the longitudinal resistivity cross-section diagram.

Abstract: A grounding grid breakpoint diagnostic method for a transient electromagnetic method, based on a transient electromagnetic detection apparatus. The method comprises: (101) disposing a test line at a position above a grounding grid to be diagnosed on the ground, and determining coordinates of a detection point; (102) obtaining information about coordinates of the detection point, and coinciding the center of the transient electromagnetic detection apparatus with the coordinates of the detection point; (103) performing, by the transient electromagnetic detection apparatus, measurement along the test line point by point, so as to obtain measurement data; (104) processing, by using diagram forming software, the measurement data to form a longitudinal resistivity cross-section diagram of the detection point according to a preset formula; (105) determining whether a breakpoint occurs at the detection point according to the longitudinal resistivity cross-section diagram.

Abstract: An inertial navigation system with error correction based on a navigation map is disclosed herein. The inertial navigation system can include a trajectory calculator for calculating a position and an orientation of a moving object based on moving information of the moving object, an error correcting unit coupled to the trajectory calculator for correcting the position and the orientation of the moving object based on reference road information, and a storage unit coupled to the error correcting unit for storing geographical information of a road network and providing the reference road information according to the geographical information of the road network.