Abstract: Solid phase extraction devices including a plurality of packed nominally aligned capillary-channeled polymeric fibers for use as stationary phase materials are disclosed. A plurality of fibers are packed together in a casing so as to provide good flow characteristics through the fibers and high surface area contact between a sample and the fibers. Different polymer compositions of the fibers permit the “chemical tuning” of the extraction process. The fibers can be physically or chemically derivatized to target specific analytes for separation from a test sample. Use of the fibers allows a wide range of liquid flow rates with very low backpressures. The fibers are easily packed into a micropipette tip or a conduit for use with a fluid flow device such as an aspirator or a pump. The devices can be used for isolation and pre-concentration of analytes from samples, for instance for proteins from buffer solutions or extraction of pollutants from remote locations.

Abstract: A novel protein was isolated from Fusarium culmorum and characterized. The protein, termed FCWP1, demonstrated significant antifungal activity against several fungal species. Mutations in proteolytic consensus sequences contained within FCWP1 improved the stability of its antifungal activity. In addition, a class of proteins related to FCWP1 was identified and characterized. This class is made up of ribosomal proteins and displayed similar values for pI and molecular weight. A representative number of proteins from this class were tested and found to have significant antifungal activities. The antifungal proteins disclosed herein are useful in controlling fungal infections in plants. Transgenic plants may be produced that are more resistant to fungal infections relative to non-transgenic plants of the same species. Alternatively, the proteins may be applied to plants exogenously.

Abstract: Transgenic plants and host cells comprising antifungal polypeptides, isolated from alfalfa plants, are shown to possess antifungal activity. DNA encoding the polypeptides was cloned into vectors for transformation of plant, therby providing a method of inhibiting fungal growth on plants.

Abstract: A novel protein was isolated from Fusarium culmorum and characterized. The protein, termed FCWP1, demonstrated significant antifungal activity against several fungal species. Mutations in proteolytic consensus sequences contained within FCWP1 improved the stability of its antifungal activity. In addition, a class of proteins related to FCWP1 was identified and characterized. This class is made up of ribosomal proteins and displayed similar values for pI and molecular weight. A representative number of proteins from this class were tested and found to have significant antifungal activities. The antifungal proteins disclosed herein are useful in controlling fungal infections in plants. Transgenic plants may be produced that are more resistant to fungal infections relative to non-transgenic plants of the same species. Alternatively, the proteins may be applied to plants exogenously.

Abstract: Antifungal polypeptides, isolated from Medicago plants, are shown to control fungal damage to plants. DNA encoding the polypeptides was cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of inhibiting fungal growth on plants. The polypeptides can be formulated into compositions useful in controlling undesired fungi.

Abstract: Antifungal polypeptides, isolated from Medicago plants, are shown to control fungal damage to plants. DNA encoding the polypeptides was cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of inhibiting fungal growth on plants. The polypeptides can be formulated into compositions useful in controlling undesired fungi.

Abstract: Antifungal polypeptides, isolated from Medicago plants, are shown to control fungal damage to plants. The polypeptides can be formulated into compositions useful in controlling undesired fungi.

Abstract: Antifungal polypeptides, isolated from Medicago plants, are shown to control fungal damage to plants. DNA encoding the polypeptides was cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of inhibiting fungal growth on plants. The polypeptides can be formulated into compositions useful in controlling undesired fungi.