Patents by Inventor Yoshifumi Jigami
Yoshifumi Jigami has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8409825Abstract: This invention provides a means for high-level secretory production of a protein, and, in particular, a protein having a complicated structure such as an antibody, in a host cell such as a yeast cell. This invention provides a method for high-level secretory production of a foreign protein with the use of a transformed host cell having one or more types of chaperone protein genes and via suppression of O sugar chain inherent to a host cell such as a yeast cell.Type: GrantFiled: October 29, 2008Date of Patent: April 2, 2013Assignees: National Institute of Advanced Industrial Science and Technology, Kyowa Hakko Kirin Co., Ltd, Daiichi Sankyo Company, LimitedInventors: Yasunori Chiba, Yoshifumi Jigami, Yoshie Takahashi, Kosuke Kuroda, Kazuo Kobayashi, Kimihisa Ichikawa, Koichi Nonaka, Takeshi Suzuki, Minako Ono
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Patent number: 8323917Abstract: The present invention enables screening for compounds that inhibit the transport of GPI-anchored proteins to fungal cell walls, using a simple assay for transacylation to GlcN-PI using membrane fraction expressing GWT1 protein. New antifungal agents can be created that inhibit the synthesis of fungal cell walls and also inhibit adhesion to host cells by inhibiting the transport of GPI-anchored proteins to fungal cell walls.Type: GrantFiled: November 21, 2003Date of Patent: December 4, 2012Assignees: National Institute of Advanced Industrial Science and Technology, Eisai R&D Management Co., Ltd.Inventors: Kappei Tsukahara, Mamiko Tsuchiya, Yoshifumi Jigami, Kenichi Nakayama, Mariko Umemura, Michiyo Okamoto
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Patent number: 8232377Abstract: This invention provides a means for enabling high-level secretory production of proteins, in particular proteins having complicated structures such as antibodies, in host cells such as yeast cells. The invention also provides transformed yeast cells having the activated HAC1 gene and the RRBP1 gene and a method for enabling high-level secretory production of foreign proteins using such transformed host cells by inhibiting O-sugar chain formation indigenous to host cells such as yeast cells.Type: GrantFiled: May 16, 2007Date of Patent: July 31, 2012Assignees: National Institute of Advanced Industrial Science and Technology, Kyowa Hakko Kirin Co., Ltd., Daiichi Sankyo Company, LimitedInventors: Yasunori Chiba, Yoshifumi Jigami, Kosuke Kuroda, Kazuo Kobayashi, Kimihisa Ichikawa, Koichi Nonaka, Takeshi Suzuki
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Patent number: 7972809Abstract: This invention is to provide a process for producing a glycoprotein comprising a mammalian type sugar chain, characterized in that the process comprises introducing an ?-1,2-mannosidase gene into a methylotrophic yeast having a mutation of a sugar chain biosynthesizing enzyme gene, so that the ?-1,2-mannosidase gene is expressed under the control of a potent promoter in the yeast; culturing in a medium the methylotrophic yeast cells with a heterologous gene transferred thereinto; and obtaining the glycoprotein comprising a mammalian type sugar chain from the culture. Using the newly created methylotrophic yeast having a sugar chain mutation, a neutral sugar chain identical with a high mannose type sugar chain produced by mammalian cells such as human cells, or a glycoprotein comprising such a neutral sugar chain, can be produced in a large amount at a high purity.Type: GrantFiled: April 28, 2003Date of Patent: July 5, 2011Assignees: National Institute of Advanced Industrial Science & Technology, Kirin Beer Kabushiki KaishaInventors: Kazuo Kobayashi, Yoshinori Kitagawa, Toshihiro Komeda, Nagako Kawashima, Yoshifumi Jigami, Yasunori Chiba
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Publication number: 20110014651Abstract: This invention provides a means for high-level secretory production of a protein, and, in particular, a protein having a complicated structure such as an antibody, in a host cell such as a yeast cell. This invention provides a method for high-level secretory production of a foreign protein with the use of a transformed host cell having one or more types of chaperone protein genes and via suppression of O sugar chain inherent to a host cell such as a yeast cell.Type: ApplicationFiled: October 29, 2008Publication date: January 20, 2011Applicants: KYOWA HAKKO KIRIN CO., LTD., DAIICHI SANKYO COMPANY, LIMITEDInventors: Yasunori Chiba, Yoshifumi Jigami, Yoshie Takahashi, Kosuke Kuroda, Kazuo Kobayashi, Kimihisa Ichikawa, Koichi Nonaka, Takeshi Suzuki, Minako Ono
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Patent number: 7803605Abstract: A method for breeding yeast having thermotolerance or recovering growth activity and a method for breeding yeast which produces beta-glucan efficiently as well as an yeast obtained by such methods for breeding are presented by a method for breeding yeast having thermotolerance or recovering growth activity including a step for controlling proofreading function of DNA polymerase in a loss-of-function mutant of yeast (for example, a step for including mutant pol3 gene or mutant cdc6? gene in a gene-disruptant.Type: GrantFiled: March 2, 2007Date of Patent: September 28, 2010Assignees: National Institute of Advanced Industrial Science and Technology, Neo-Morgan Laboratory IncorporatedInventors: Hiroko Abe, Kenichi Nakayama, Yoshifumi Jigami, Yasunori Chiba, Yuki Takaoka, Akiko Itadani
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Patent number: 7741087Abstract: This invention provides proteins and genes thereof involved in the GPI lipid remodeling process and, thereby and constructing a system for screening for useful substances such as anticancer agents and a system for detecting abnormalities in the GPI lipid remodeling process.Type: GrantFiled: August 7, 2007Date of Patent: June 22, 2010Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Yoshifumi Jigami, Takehiko Yoko-O, Mariko Umemura, Morihisa Fujita
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Patent number: 7579166Abstract: A process for producing a lysosomal enzyme having a mannose-6-phosphate-containing acidic sugar chain, wherein the process comprising: culturing in a medium yeast cells obtained by introducing a lysosomal enzyme gene into a sugar chain biosynthetic enzyme gene mutant strain of yeast, collecting a lysosomal enzyme having a phosphate-containing sugar chain from the culture, and then treating the enzyme with ?-mannosidase; and pharmaceutical compositions for treatment of human lysosomal enzyme deficiencies produced by the process. The genetic engineering technique using the yeast according to the present invention allows large-amount and high-purity production of a glycoprotein having a phosphate-containing acidic sugar chain which can serve as a labeling marker for transporting into lysosomes in cells of mammals such as human. The glycoprotein having a phosphate-containing acidic sugar chain according to the invention may be utilized as a drug effective in treatment of human lysosomal enzyme deficiencies, etc.Type: GrantFiled: June 14, 2002Date of Patent: August 25, 2009Assignees: National Institute of Advanced Industrial Science and Technology, Tokyo Metropolitan Organization for Medical ResearchInventors: Yasunori Chiba, Yoshifumi Jigami, Hitoshi Sakuraba, Kazuo Kobayashi, Makoto Takeuchi, Yoriko Takeuchi, legal representative
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Publication number: 20090191587Abstract: This invention provides a means for enabling high-level secretory production of proteins, in particular proteins having complicated structures such as antibodies, in host cells such as yeast cells. The invention also provides transformed yeast cells having the activated HAC1 gene and the RRBP1 gene and a method for enabling high-level secretory production of foreign proteins using such transformed host cells by inhibiting O-sugar chain formation indigenous to host cells such as yeast cells.Type: ApplicationFiled: May 16, 2007Publication date: July 30, 2009Inventors: Yasunori Chiba, Yoshifumi Jigami, Kosuke Kuroda, Kazuo Kobayashi, Kimihisa Ichikawa, Koichi Nonaka, Takeshi Suzuki
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Publication number: 20090068702Abstract: The present invention relates to a means for generating a mucin-type glycopeptide or glycoprotein on a large scale in yeast. Specifically, the invention relates to a method which comprises introducing into a yeast at least one selected from the group consisting of a gene encoding UDP-GalNAc synthetase, a gene encoding UDP-GalNAc transporter, and a gene encoding polypeptide:O-GalNAc transferase, and, if desired, a gene encoding a mucin-type glycopeptide; and producing a mucin-type glycoprotein having O-GalNAc by use of the yeast.Type: ApplicationFiled: February 26, 2007Publication date: March 12, 2009Inventors: Yasunori CHIBA, Kou Amano, Yoshifumi Jigami, Kazuo Kobayashi
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Publication number: 20080233608Abstract: This invention provides proteins and genes thereof involved in the GPI lipid remodeling process and, thereby and constructing a system for screening for useful substances such as anticancer agents and a system for detecting abnormalities in the GPI lipid remodeling process.Type: ApplicationFiled: August 7, 2007Publication date: September 25, 2008Inventors: Yoshifumi Jigami, Takehiko Yoko-O, Mariko Umemura, Morihisa Fujita
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Publication number: 20080064069Abstract: A protein having (a) an amino acid sequence represented by SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 20, SEQ ID NO: 6 or SEQ ID NO: 8, or (b) an amino acid sequence represented by SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 20, SEQ ID NO: 6 or SEQ ID NO: 8, wherein one or more amino acids are deleted, substituted and/or added in the amino acid sequence set forth in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 20, SEQ ID NO: 6 or SEQ ID NO: 8, and said sequence has a UDP-glucose 4,6-dehydratase activity, and/or UDP-4-keto-6-deoxyglucose 3,5-epimerase and UDP-4-keto-rhamnose 4-keto-reductase activities; and a method of producing UDP-rhamnose using the above-described protein.Type: ApplicationFiled: May 22, 2007Publication date: March 13, 2008Applicant: National Institute of Advanced Industrial Science and TechnologyInventors: Takuji Oka, Yoshifumi Jigami
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Publication number: 20080038778Abstract: A method for breeding yeast having thermotolerance or recovering growth activity and a method for breeding yeast which produces beta-glucan efficiently as well as an yeast obtained by such methods for breeding are presented by a method for breeding yeast having thermotolerance or recovering growth activity including a step for controlling proofreading function of DNA polymerase in a loss-of-function mutant of yeast (for example, a step for including mutant pol3 gene or mutant cdc6?gene in a gene-disruptant.Type: ApplicationFiled: March 2, 2007Publication date: February 14, 2008Applicants: National Institute of Advanced Industrial Science and Technology, Neo-Morgan Laboratory IncorporatedInventors: Hiroko Abe, Kenichi Nakayama, Yoshifumi Jigami, Yasunori Chiba, Yuki Takaoka, Akiko Itadani
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Patent number: 7217548Abstract: The present invention relates to an enzyme having novel N-acetylglucosaminyltransferase (OMGnT) activity, a polynucleotide encoding the enzyme, a recombinant polynucleotide comprising the polynucleotide, a host cell comprising the recombinant polynucleotide, a method for producing an enzyme protein having OMGnT activity by culturing the host cell in a medium, and a method for modifying a sugar chain structure with the enzyme and a carbohydrate having a sugar chain structure modified by the method. This enzyme enables the production of complex carbohydrates, which could not be formed with conventional glycosyltransferases.Type: GrantFiled: October 5, 2001Date of Patent: May 15, 2007Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Aruto Yoshida, Yoriko Takeuchi, legal representative, Tamao Endo, Hiroshi Manya, Yasunori Chiba, Yoshifumi Jigami, Shigemi Sugioka, Makoto Takeuchi, deceased
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Patent number: 7198932Abstract: The present invention relates to a gene for an enzyme involving in the synthesis of GDP-L-fucose. Particularly, the present invention relates to a GDP-4-keto-6-deoxy-D-mannose-3, 5-epimerase-4-reductase gene derived from Arabidopsis thaliana, and a process for producing GDP-L-fucose using the gene. An enzyme encoded by the gene is (a) a protein comprising an amino acid sequence represented by SEQ ID NO: 1; or (b) a protein comprising an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by deletion, substitution, addition or insertion of one or several amino acid residues, and having GDP-4-keto-6-deoxy-D-mannose-3, 5-epimerase-4-reductase activity. The present invention enables efficient mass production of GDP-L-fucose which is essential in performing addition of fucose, which has a very important function in sugar chains.Type: GrantFiled: March 30, 2000Date of Patent: April 3, 2007Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Ken-ichi Nakayama, Yoshifumi Jigami
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Publication number: 20060240429Abstract: The present invention enables screening for compounds that inhibit the transport of GPI-anchored proteins to fungal cell walls, using a simple assay for transacylation to GlcN-PI using membrane fraction expressing GWT1 protein. New antifungal agents can be created that inhibit the synthesis of fungal cell walls and also inhibit adhesion to host cells by inhibiting the transport of GPI-anchored proteins to fungal cell walls.Type: ApplicationFiled: November 21, 2003Publication date: October 26, 2006Inventors: Kappei Tsukahara, Mamiko Tsuchiya, Yoshifumi Jigami, Kenichi Nakayama, Mariko Umemura, Michiyo Okamoto
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Publication number: 20060148039Abstract: This invention is to provide a process for producing a glycoprotein comprising a mammalian type sugar chain, characterized in that the process comprises introducing an ?-1,2-mannosidase gene into a methylotrophic yeast having a mutation of a sugar chain biosynthesizing enzyme gene, so that the ?-1,2-mannosidase gene is expressed under the control of a potent promoter in the yeast; culturing in a medium the methylotrophic yeast cells with a heterologous gene transferred thereinto; and obtaining the glycoprotein comprising a mammalian type sugar chain from the culture. Using the newly created methylotrophic yeast having a sugar chain mutation, a neutral sugar chain identical with a high mannose type sugar chain produced by mammalian cells such as human cells, or a glycoprotein comprising such a neutral sugar chain, can be produced in a large amount at a high purity.Type: ApplicationFiled: April 28, 2003Publication date: July 6, 2006Inventors: Kazuo Kobayashi, Yoshinori Kitagawa, Toshihiro Komeda, Nagako Kawashima, Yoshifumi Jigami, Yasumori Chiba
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Publication number: 20060099680Abstract: An object of the present invention is to provide a means for producing O-fucosylated protein in large quantities, a means for searching for new genes associated with the synthesis system of O-fucosylated protein or the proteins expressed by the genes, and a means for elucidating the functions thereof. According to the present invention, a yeast transformant characterized in that the genes associated with the synthesis system of O-fucosylated protein are a GDP-fucose synthase gene, a GDP-fucose transporter gene, a fucosyltransferase gene, and a fucose receptor gene is provided.Type: ApplicationFiled: April 25, 2005Publication date: May 11, 2006Applicant: Nat Inst of Adv Industrial Sci and TechInventors: Yoshifumi Jigami, Yuuko Chigira, Xiao-Dong Gao, Si jun Dong
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Publication number: 20050064539Abstract: A process for producing a lysosomal enzyme having a mannose-6-phosphate-containing acidic sugar chain, wherein the process comprising: culturing in a medium yeast cells obtained by introducing a lysosomal enzyme gene into a sugar chain biosynthetic enzyme gene mutant strain of yeast, collecting a lysosomal enzyme having a phosphate-containing sugar chain from the culture, and then treating the enzyme with ?-mannosidase; and pharmaceutical compositions for treatment of human lysosomal enzyme deficiencies produced by the process. The genetic engineering technique using the yeast according to the present invention allows large-amount and high-purity production of a glycoprotein having a phosphate-containing acidic sugar chain which can serve as a labeling marker for transporting into lysosomes in cells of mammals such as human. The glycoprotein having a phosphate-containing acidic sugar chain according to the invention may be utilized as a drug effective in treatment of human lysosomal enzyme deficiencies, etc.Type: ApplicationFiled: June 14, 2002Publication date: March 24, 2005Inventors: Yasunori Chiba, Yoshifumi Jigami, Hitoshi Sakuraba, Kazuo Kobayashi, Makoto Takeuchi, Yoriko Takeuchi
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Publication number: 20040132130Abstract: The present invention relates to an enzyme having novel N-acetylglucosaminyltransferase (OMGnT) activity, a polynucleotide encoding the enzyme, a recombinant polynucleotide comprising the polynucleotide, a host cell comprising the recombinant polynucleotide, a method for producing an enzyme protein having OMGnT activity by culturing the host cell in a medium, and a method for modifying a sugar chain structure with the enzyme and a carbohydrate having a sugar chain structure modified by the method. This enzyme enables the production of complex carbohydrates, which could not be formed with conventional glycosyltransferases.Type: ApplicationFiled: September 15, 2003Publication date: July 8, 2004Inventors: Aruto Yoshida, Makoto Takeuchi, Yoriko Takeuchi, Tamao Endo, Hirosi Manya, Yasunori Chiba, Yoshifumi Jigami, Shigemi Sugioka