Abstract: A metal filter includes a plurality of through holes. An opening shape of each of the plurality of through holes is a rectangle or rounded-rectangle and a length of a short side of the rectangle or a rounded rectangle is 5 to 15 ?m. A method for concentrating cancer cells using the metal filter includes separating cancer cells from blood and blood cell constituents by passing blood, red blood cells, white blood cells, and platelets through the plurality of through holes while capturing cancer cells to concentrate the cancer cells.

Abstract: A cell trapping device 1 includes: a housing 10, a filter, and a plurality of fastening members 60. The housing 10 includes: a lid member 100 having an introduction channel 101 configured to introduce a test liquid into an inside; and a storage member 200 having a discharge channel 201 configured to discharge the test liquid to an outside. The filter is provided on a channel in the housing 10 and supported between the lid member 100 and the storage member 200 by the lid member 100 and the storage member 200. The fastening members 60 configured to clamp the lid member 100 and the storage member 200 from both sides in an intersecting direction that is a direction intersecting with the filter to fasten the lid member 100 and the storage member 200 to each other.

Abstract: Provided are a cancer cell-trapping metal filter which has a high opening ratio, a cancer cell-trapping metal filter sheet, a cancer cell-trapping device using the cancer cell-trapping filter, and manufacturing methods therefor. According to a cancer cell-trapping metal filter 1, openings of connected through-holes 12 that are formed in a metal sheet 11 have a wave shape, and thus it is possible to extract a CTC from other components by utilizing a hole diameter on a short-side side of the openings, and it is possible to make the connected through-holes be closer to each other due to the wave shape while maintaining a CTC trapping ability. Accordingly, it is possible to further improve the opening ratio in the cancer cell-trapping metal filter 1.

Abstract: A cell-capturing device includes: one or more introduction channels for introducing test liquid or treatment liquid; a discharge channel for discharging the test liquid or the treatment liquid to the outside; a filter having a plurality of through-holes, and being disposed in a channel so that the test liquid or the treatment liquid passes through the through-holes; an introduction region formed between the filter and the introduction channel in a channel; a discharge region formed between the filter and the discharge channel; and a housing part accommodating at least a part of the introduction channel, the introduction region, and the discharge region therein, wherein the cell-capturing device further includes a pre-treatment part formed at a position apart from a connection part with the introduction region on at least one of the introduction channels, and formed by a spatial region having a diameter larger than that of the introduction channel.

Abstract: Provided is a cell trapping method for selectively trapping a specific cell included in a cell-containing solution at a filter surface by filtering a liquid, and the method includes a step of draining a liquid for n (n is a natural number) times, in which from the first step of draining the liquid to the n-th step of draining the liquid, a liquid surface of the liquid in the introduction region on the filter is maintained at a predetermined liquid surface height, and when the n-th step of draining the liquid is completed, the discharging of the liquid from the cell trapping device is stopped in a state where the liquid surface is at a predetermined height in the filter.

Abstract: Provided are a cancer cell-trapping metal filter which has a high opening ratio, a cancer cell-trapping metal filter sheet, a cancer cell-trapping device using the cancer cell-trapping filter, and manufacturing methods therefor. According to a cancer cell-trapping metal filter 1, openings of connected through-holes 12 that are formed in a metal sheet 11 have a wave shape, and thus it is possible to extract a CTC from other components by utilizing a hole diameter on a short-side side of the openings, and it is possible to make the connected through-holes be closer to each other due to the wave shape while maintaining a CTC trapping ability. Accordingly, it is possible to further improve the opening ratio in the cancer cell-trapping metal filter 1.

Abstract: By providing a pre-processing unit on a processing liquid channel included in a cell capturing apparatus, air bubbles accumulate in an introduction region inside of the pre-processing unit and thus the air bubbles are removed while a processing liquid moves through the introduction region and a discharge region, even when the air bubbles are contained in the processing liquid. By providing a foreign matter removal filter, at least a part of foreign matters (foreign matters that cannot pass through through-holes) contained in the processing liquid can be captured and thus the processing liquid from which the foreign matters are removed can be supplied to a cell capturing device at the subsequent stage.

Abstract: A metal filter includes a plurality of through holes. An opening shape of each of the plurality of through holes is a rectangle or rounded-rectangle and a length of a short side of the rectangle or a rounded rectangle is 5 to 15 ?m. A method for concentrating cancer cells using the metal filter includes separating cancer cells from blood and blood cell constituents by passing blood, red blood cells, white blood cells, and platelets through the plurality of through holes while capturing cancer cells to concentrate the cancer cells.

Abstract: Cells in a test liquid are captured and observed easily and accurately. A cell capture filter 30 is fixed by a first protrusion part 125 of a lid member 100 and a second protrusion part 225 of a housing member 200 in a cell capture device 1, and a first fitting part 130 and a second fitting part 230 are fitted with each other. This allows the cell capture filter 30 to be fixed between the lid member 100 and the housing member 200 in a state where a filter region of the cell capture filter 30 is surrounded by the looped first protrusion part 125 and the looped second protrusion part 225.

Abstract: A cancer cell isolation method isolates cancer cells trapped on a filter without any damage. In a cancer cell isolation device, cancer cells X trapped on a filter are captured by a camera as an image capturing unit, and the cancer cells X are carried one by one by using a handling unit while the filter and a storage container are moved by an X-axis movement stand, a Y-axis movement stand, and a Z-axis movement stand as movement units while the captured image is output from an output unit. Accordingly, it is possible to isolate the cancer cells on the filter without damaging the cancer cells and hence to appropriately perform an observation or the like.

Abstract: A cell trapping device 1 includes: a housing 10, a filter, and a plurality of fastening members 60. The housing 10 includes: a lid member 100 having an introduction channel 101 configured to introduce a test liquid into an inside; and a storage member 200 having a discharge channel 201 configured to discharge the test liquid to an outside. The filter is provided on a channel in the housing 10 and supported between the lid member 100 and the storage member 200 by the lid member 100 and the storage member 200. The fastening members 60 configured to clamp the lid member 100 and the storage member 200 from both sides in an intersecting direction that is a direction intersecting with the filter to fasten the lid member 100 and the storage member 200 to each other.

Abstract: Disclosed is to isolate cancer cells trapped on a filter without any damage. In a cancer cell isolation device (1), cancer cells X trapped on a filter (100) are captured by a camera (30) as an image capturing unit, and the cancer cells X are carried one by one by using a handling unit (40) while the filter (100) and a storage container (200) are moved by an X-axis movement stand (14), a Y-axis movement stand (16), and a Z-axis movement stand (18) as movement units while the captured image is output from an output unit (32). Accordingly, it is possible to isolate the cancer cells on the filter without damaging the cancer cells and hence to appropriately perform an observation or the like.