Abstract: An Escherichia bacterium having dihydrodipicolinate synthase and aspartokinase, both of which are desensitized to feedback inhibition by L-lysine. The intracellular activity of dihydrodipicolinate reductase in this bacterium can also be enhanced. Furthermore, a diaminopimelate dehydrogenase gene can be introduced into this bacterium, or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase can be enhanced. Finally, the intracellular activities of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase can be enhanced in this bacterium. The bacterium can be cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.

Abstract: The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.

Abstract: An Escherichia bacterium (1) which harbors dihydrodipicolinate synthase of which feedback inhibition by L-lysine is desensitized and aspartokinase of which feedback inhibition by L-lysine is desensitized, (2) in which intracellular activity of dihydrodipicolinate reductase is enhanced, and (3) in which a diaminopimelate dehydrogenase gene is introduced or intracellular activities of tetrahydrodipicolinate succinylase and succinyl diaminopimelate deacylase are enhanced, wherein intracellular activity of aspartate-semialdehyde dehydrogenase or phosphoenolpyruvate carboxylase is enhanced, is cultured in a suitable medium to produce and accumulate L-lysine in culture, and the L-lysine is collected from the culture.

Abstract: The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium. According to the present invention, a process is provided for the secretory production of a foreign protein, in particular, transglutaminase, by making a coryneform bacterium to produce an industrially useful foreign protein, in particular, transglutaminase and efficiently release the product extracellularly (i.e., secretory production).

Abstract: The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.

Abstract: It is an object of the present invention to obtain an ultrathin angle detector by disposing an annular rotor board face to face with stator windings of a plurality of stators provided on an annular stator board. An angle detector according to the present invention is constructed by providing magnetic cores and the stator windings on the respective stators provided on a surface of the annular stator board via slots, and disposing a corrugated curved surface formed on a board inner surface of the annular rotor board face to face with the stators so as to obtain an angular signal that changes according to a sinusoidal waveform.

Abstract: In an angle detector according to the present invention, a magnetic rotor is rotated while being face-to-face between a pair of magnetic stator strips of each of magnetic stator portions provided separately to an annular stator frame, so high-accuracy detection is carried out irrespective of decentering of a rotary shaft.

Abstract: By culturing a methanol-assimilating bacterium which harbors a DNA construct which contains a promoter sequence that functions in the methanol-assimilating bacterium and a nucleotide sequence that encodes a polypeptide containing a signal sequence and an objective protein which is functionally connected to the promoter sequence, in a liquid medium containing methanol as a major carbon source, the bacterium is allowed to secrete the objective protein, and the secreted objective protein is recovered.

Abstract: The present invention provides a method for efficiently producing an industrially useful protein in coryneform bacteria, and more particularly, a method for efficiently producing a protein for which secretion was difficult using conventional protein secretion pathways. In particular, the present invention provides a method for efficiently producing heterologous proteins comprising culturing coryneform bacteria containing an genetic construction containing a promoter sequence which functions in coryneform bacteria, a nucleic acid sequence encoding a Tat system-dependent signal peptide region, and a nucleic acid sequence encoding a heterologous protein, in the direction from 5?-end to 3?-end, and secretory producing the heterologous protein by coryneform bacteria.

Abstract: The object of the present invention is to provide a method of producing a heterologous protein by making a coryneform bacterium to produce and efficiently extracellularly secrete (secreto-production) an industrially useful heterologous protein. According to the present invention, a genetic construct is used where a gene sequence encoding an intended protein which is ligated to the downstream of a sequence encoding the signal peptide derived from a coryneform bacterium, the gene construct is introduced into a mutant coryneform bacterium which has a capacity of secreting the heterologous protein at least 2-fold higher than the wild type Corynebacterium glutamicum ATCC 13869, the mutant coryneform bacterium is cultured and the extracellularly released heterologous protein is recovered.

Abstract: The present invention provides a neutral metalloprotease from actinomycetes which selectively cleaves a pro-structure part of a microbial protransglutaminase and a gene encoding said neutral metalloprotease. An active microbial transglutaminase having the pro-structure part cleaved can be obtained by culturing a microorganism into which a gene encoding the neutral metalloprotease from actinomycetes according to the present invention has been introduced, where by producing the neutral metalloprotease from actinomycetes, and reacting it on a microbial protransglutaminase.

Abstract: The object of the present invention is to provide a method of producing a heterologous protein by making a coryneform bacterium to produce and efficiently extracellularly secrete (secreto-production) an industrially useful heterologous protein. According to the present invention, a genetic construct is used where a gene sequence encoding an intended protein which is ligated to the downstream of a sequence encoding the signal peptide derived from a coryneform bacterium, the gene construct is introduced into a mutant coryneform bacterium which has a capacity of secreting the heterologous protein at least 2-fold higher than the wild type Corynebacterium glutamicum ATCC 13869, the mutant coryneform bacterium is cultured and the extracellularly released heterologous protein is recovered.

Abstract: A guanidine derivative containing an amido group, represented by 1

Abstract: A recombinant wherein an arginine decarboxylase gene (adi) was amplified and expressed is created by genetic engineering means, and the recombinant, or arginine decarboxylase or an arginine decarboxylase-containing material obtained from the recombinant, is used to decarboxylate arginine to produce agmatine.

Abstract: An automatic balancing apparatus may be mounted in a rotator body such as a spindle motor having a rotary shaft defining an axis of rotation. The automatic balancing apparatus has a plurality of balancing members rotatable about the axis of rotation in a non-contact manner. Each of the balancing members has a balance action section spaced a distance in a radial direction from the axis of rotation and a connection section that rotatably couples the balance action section to the axis of rotation. The balance action section has a mass that effects a balance correction action to cancel a rotational unbalance of the rotator body when the rotator body rotates at a rotational speed that exceeds a resonant rotational speed CR thereof. The connection section has a mass that substantially does not affect the balance correction action compared to the balance action section.

Abstract: A method for producing a fermentative product by utilizing a microorganism, the method comprising culturing the microorganism in a medium to produce and accumulate the fermentative product in the medium, and collecting the fermentative product, wherein the microorganism expresses a heat shock protein derived from a hyperthermophilic archaeon strain KOD-1 in a cell of the microorganism by introduction of a gene coding for the heat shock protein.

Abstract: The present invention relates to a process for secretory production of a foreign protein, in particular, transglutaminase by a coryneform bacterium.

Abstract: A cylindrical case provided with a chamber is coaxially attached to a rotary shaft of a motor. A plurality of balancers are movably provided within the chamber so as to cancel a rotation eccentricity of the motor. A viscous member such as high viscosity fluid is provided on at least one of the surfaces of the balancers and an inner face of the chamber.

Abstract: An automatic balancing apparatus which includes a support shaft which is rotated by a drive; a case body which is fixed to the support shaft and which rotates together with the support shaft; a ring magnet which is placed at the inside corner, in the radial direction, of an inner space of the case body; and a balancing member. The balancing member further includes a plurality of magnetic bodies which are movable in the inner space of the case body and which can be drawn up by the magnetic field of the ring magnet. When the rotational frequency of a rotor, which rotates together with the case body, exceeds the resonant frequency, the automatic balancing apparatus reduces vibrations of the rotor; and the outer circumference of the ring magnet has a flat portion.

Abstract: An automatic balancing apparatus may be mounted in a rotator body such as a spindle motor having a rotary shaft defining an axis of rotation. The automatic balancing apparatus has a plurality of balancing members rotatable about the axis of rotation in a non-contact manner. Each of the balancing members has a balance action section spaced a distance in a radial direction from the axis of rotation and a connection section that rotatably couples the balance action section to the axis of rotation. The balance action section has a mass that effects a balance correction action to cancel a rotational unbalance of the rotator body when the rotator body rotates at a rotational speed that exceeds a resonant rotational speed CR thereof. The connection section has a mass that substantially does not affect the balance correction action compared to the balance action section.