Abstract: Disclosed are a composition for gene transfer, a method for introducing a gene into cell of a non-human animal, a method for introducing a gene into an in vitro cell, and a kit for gene transfer. The composition comprises (A) at least one lipid selected from the group consisting of sorbitan sesquioleate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan trioleate, sorbitan monooleate, L-?-phosphatidylinositol, L-?-dioleoyl phosphatidylethanolamine, octadecylamine, hexadecylamine, DOTAP, and cardiolipin; (B) at least one protein selected from the group consisting of albumin, casein, gelatin, and sericin; and (C) at least one positively charged substance selected from the group consisting of protamine sulfate, polyarginine, polylysine, polyethyleneimine, and hexadimethrine bromide.

Abstract: This invention relates to a monoclonal antibody against a human HIG-1 polypeptide, the antibody binding to at least one epitope included in the amino acid sequence at positions 1-19 of a human HIG-1 polypeptide; an antibody fragment derived from the antibody; a DNA comprising a base sequence encoding a variable region of the antibody; an expression vector comprising the DNA; a cell line producing the antibody; a reagent for detecting a human HIG-1 polypeptide comprising the antibody or the antibody fragment, and a method for detecting a human HIG-1 polypeptide using the antibody or the antibody fragment.

Abstract: Delivery proteins are provided for transferring a protein, antibody or foreign substance into a cell without impairing the function or structure thereof. Further, methods of transferring a foreign substance into a cell at a high efficiency by using the delivery protein or an envelope virus or inactivated envelope virus in combination with said delivery protein are provided. The inventors discovered that a protein containing a polypeptide having an affinity for a constituent of the envelope virus contributes to the efficient enclosure of the foreign substance in the envelope. Moreover, the inventors discovered that use of the delivery protein enables foreign substances to be included in an envelope virus or inactivated envelope virus and therefore makes it possible to efficiently transfer the substances into cells without damaging the physiological function thereof.

Abstract: Delivery proteins are provided for transferring a protein, antibody or foreign substance into a cell without impairing the function or structure thereof. Further, methods of transferring a foreign substance into a cell at a high efficiency by using the delivery protein or an envelope virus or inactivated envelope virus in combination with said delivery protein are provided. The inventors discovered that a protein containing a polypeptide having an affinity for a constituent of the envelope virus contributes to the efficient enclosure of the foreign substance in the envelope. Moreover, the inventors discovered that use of the delivery protein enables foreign substances to be included in an envelope virus or inactivated envelope virus and therefore makes it possible to efficiently transfer the substances into cells without damaging the physiological function thereof.

Abstract: This invention relates to a monoclonal antibody against a human HIG-1 polypeptide, the antibody binding to at least one epitope included in the amino acid sequence at positions 1-19 of a human HIG-1 polypeptide; an antibody fragment derived from the antibody; a DNA comprising a base sequence encoding a variable region of the antibody; an expression vector comprising the DNA; a cell line producing the antibody; a reagent for detecting a human HIG-1 polypeptide comprising the antibody or the antibody fragment, and a method for detecting a human HIG-1 polypeptide using the antibody or the antibody fragment.

Abstract: Proteins are provided for transferring a protein, an antibody or another foreign substance into a cell without impairing the function or structure thereof; and methods of transferring a foreign substance into a cell in a time and quantity controllable manner at a high efficiency by using the above-described delivery protein or an envelope virus or inactivated envelope virus in combination with said delivery protein. As the results of intensive studies on a method of enclosing a foreign substance in the envelope of an envelope virus, it is found out that a protein containing a polypeptide having an affinity for a constituent of the envelope virus contributes to the efficient enclosure of the foreign substance in the envelope.

Abstract: An annular bearing has its bearing surface on the inner surface. The bearing surface is formed to improve bearing properties, that is, to improve adaptability to an impact load and an imbalanced load. A method of manufacturing a bearing having such a bearing surface is given. An annular bearing blank, together with a mandrel having a plurality of arch-shaped ridges extending axially on the outer surface of the mandrel and a sleeve fitted on a periphery of the mandrel, is pushed into an inner hole of a molding die. The material is accordingly closed in a space defined by the mandrel, the inner hole, the sleeve and an ejector inserted into the bottom part of the inner hole. Thus, a plurality of arch-shaped recesses are formed extending axially on the inner surface of the bearing blank.

Abstract: An annular bearing has its bearing surface on the inner surface. The bearing surface is formed to improve bearing properties, that is, to improve adaptability to an impact load and an imbalanced load. A method of manufacturing a bearing having such a bearing surface is given. An annular bearing blank, together with a mandrel having a plurality of arch-shaped ridges extending axially on the outer surface of the mandrel and a sleeve fitted on a periphery of the mandrel, is pushed into an inner hole of a molding die. The material is accordingly closed in a space defined by the mandrel, the inner hole, the sleeve and an ejector inserted into the bottom part of the inner hole. Thus, a plurality of arch-shaped recesses are formed extending axially on the inner surface of the bearing blank.

Abstract: A bushing having an outer sliding surface is produced by a die forming method without machining. A crowning shape is provided to the outer surface so that the outer diameter of the bushing is smaller at its opposite longitudinal ends, and increases progressively toward a central zone of the bushing. A cylindrical bushing blank is fitted on distal end portion of a vertically-movable mandrel. Next, one of an upper die and a lower die is reactively moved toward the other to plastically work the bushing blank by the both dies and the mandrel, thereby working the bushing. An inner diameter of the upper die is larger at its lower end, and decreases progressively away from the lower end, and an inner diameter of the lower die is larger at its upper end, and decreases progressively away from the upper end.

Abstract: An inner sliding surface layer of a bearing bushing is formed into a crowning shape in an axial direction by a die shaping method. A bushing blank fitted on a generally-tapered lower end portion of an upper mandrel is inserted, together with the upper mandrel, into a hole in a forming die, and the bushing blank is deformed between the forming die, the upper mandrel and a lower mandrel having an upper end portion similar in shape to the lower end portion of the upper mandrel, thereby obtaining the bushing having the sliding inner peripheral surface of a crowning shape.

Abstract: An NC lathe with a robot for automatically exchanging works and chuck claws comprising a numerically controlled lathe having a rotatable chuck, a robot disposed in the vicinity of the numerically controlled lathe, and a feeder disposed in the vicinity of the robot. Groups of works and magazines containing chuck claws are placed on the pallets carried on the feeder, forming respective sets. The robot executes the exchanging operation of the works and the chuck claws between the feeder and the chuck.