Patents by Inventor Youming Zhang
Youming Zhang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20120208277Abstract: The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.Type: ApplicationFiled: January 3, 2012Publication date: August 16, 2012Applicant: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Francis STEWART, Youming ZHANG, Frank BUCHHOLZ
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Publication number: 20100317061Abstract: The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.Type: ApplicationFiled: April 9, 2010Publication date: December 16, 2010Applicant: EUROPAISCHES LABORATORIUM FUR MOLEKULARBIOLOGIE (EMBL)Inventors: FRANCIS STEWART, YOUMING ZHANG, FRANK BUCHHOLZ
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Patent number: 7736851Abstract: The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.Type: GrantFiled: May 11, 2004Date of Patent: June 15, 2010Assignee: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Francis Stewart, Youming Zhang, Frank Buchholz
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Publication number: 20090298117Abstract: The invention provides a method for the heterologous expression of a secondary metabolite encoded by a biosynthetic pathway. Also provided is a method for introducing a large sized DNA molecule into the chromosome of a heterologous host using a transposable element. Novel myxochromide S derivatives are also provided.Type: ApplicationFiled: October 26, 2005Publication date: December 3, 2009Inventors: Youming Zhang, Rolf Müller, Francis Stewart, Frank Gross, Silke C. Wenzel, Jun Fu
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Publication number: 20090007280Abstract: The present invention relates to isolated nucleic acid sequences of ANGE, CLLD8 and CLLD7 or sequences complementary or substantially homologous thereto or fragments thereof. Also provided are sequences comprising hybrid nucleic acid sequences from two or more of the genes. Also provided are nucleic acid expression vectors, polypeptides, antibodies to the polypeptides, host cells, non-human transgenic animals and pharmaceutical compositions and agents. Also provided is the use of the nucleic acid sequence and/or protein in medicine and research, methods for diagnosing or determining predisposition to disease or severity of disease, methods for preventing or treating disease, and kits for use in the methods and the use of the nucleic acid sequence and protein in treating or preventing IgE mediated diseases and non-atopic asthma, and in screens for identifying new agents for use in the methods.Type: ApplicationFiled: March 11, 2008Publication date: January 1, 2009Inventors: Youming Zhang, Miriam Moffatt, William Cookson, Jon Tinsley
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Patent number: 7364864Abstract: The present invention relates to isolated nucleic acid sequences of ANGE, CLLD8 and CLLD7 or sequences complementary or substantially homologous thereto or fragments thereof. Also provided are sequences comprising hybrid nucleic acid sequences from two or more of the genes. Also provided are nucleic acid expression vectors, polypeptides, antibodies to the polypeptides, host cells, non-human transgenic animals and pharmaceutical compositions and agents. Also provided is the use of the nucleic acid sequence and/or protein in medicine and research, methods for diagnosing or determining predisposition to disease or severity of disease, methods for preventing or treating disease, and kits for use in the methods and the use of the nucleic acid sequence and protein in treating or preventing IgE mediated diseases and non-atopic asthma, and in screens for identifying new agents for use in the methods.Type: GrantFiled: November 3, 2005Date of Patent: April 29, 2008Assignee: Isis Innovation LimitedInventors: Youming Zhang, Miriam Moffatt, William Cookson, Jon Tinsley
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Publication number: 20060257928Abstract: The present invention relates to isolated nucleic acid sequences of ANGE, CLLD8 and CLLD7 or sequences complementary or substantially homologous thereto or fragments thereof. Also provided are sequences comprising hybrid nucleic acid sequences from two or more of the genes. Also provided are nucleic acid expression vectors, polypeptides, antibodies to the polypeptides, host cells, non-human transgenic animals and pharmaceutical compositions and agents. Also provided is the use of the nucleic acid sequence and/or protein in medicine and research, methods for diagnosing or determining predisposition to disease or severity of disease, methods for preventing or treating disease, and kits for use in the methods and the use of the nucleic acid sequence and protein in treating or preventing IgE mediated diseases and non-atopic asthma, and in screens for identifying new agents for use in the methods.Type: ApplicationFiled: November 3, 2005Publication date: November 16, 2006Inventors: Youming Zhang, Miriam Moffatt, William Cookson, Jon Tinsley
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Patent number: 7083976Abstract: The present invention is directed to methods and compositions for TnpI-mediated genetic engineering using the Bacillus thuringiensis recombinase TnpI and the TnpI recombination substrates TRT or TRT?, and variants thereof. In particular, the invention relates to TRT or TRT? sequences, and variants thereof, vectors, cells and kits useful for TnpI-mediated genetic recombination, as well as methods for the use of TRT or TRT? sequences for TnpI-mediated genetic engineering.Type: GrantFiled: June 30, 2001Date of Patent: August 1, 2006Assignees: The European Molecular Biology Laboratory, l 'Universite catholique de LouvainInventors: A. Francis Stewart, Youming Zhang, Bernard Hallet
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Patent number: 6930094Abstract: The present invention relates to the use of tissue factor for influencing blood vessel formation, particularly for activating blood vessel formation, above all for wound healing.Type: GrantFiled: May 8, 1998Date of Patent: August 16, 2005Assignee: Merckle GmbHInventors: Peter Nawroth, Katsumi Nakagawa, Youming Zhang
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Publication number: 20050085627Abstract: The present invention relates to isolated nucleic acid sequences of ANGE, CLLD8 and CLLD7 or sequences complementary or substantially homologous thereto or fragments thereof. Also provided are sequences comprising hybrid nucleic add sequences from two or more of the genes. Also provided are nucleic acid expression vectors, polypeptides, antibodies to the polypeptides, host cells, non-human transgenic animals and pharmaceutical compositions and agents. Also provided is the use of the nucleic acid sequence and/or protein in medicine and research, methods for diagnosing or determining predisposition to disease or severity of disease, methods for preventing or treating disease, and kits for use in the methods and the use of the nucleic acid sequence and protein in treating or preventing IgE mediated diseases and non-atopic asthma, and in screens for identifying new agents for use in the methods.Type: ApplicationFiled: June 21, 2002Publication date: April 21, 2005Inventors: Youming Zhang, Miriam Moffatt, William Cookson, Jon Tinsley
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Publication number: 20040203057Abstract: The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.Type: ApplicationFiled: May 11, 2004Publication date: October 14, 2004Applicant: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Francis Stewart, Youming Zhang, Frank Buchholz
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Patent number: 6787316Abstract: The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.Type: GrantFiled: August 30, 2002Date of Patent: September 7, 2004Assignee: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Francis Stewart, Youming Zhang, Frank Buchholz
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Publication number: 20040101520Abstract: The invention relates to a novel method for altering the sequence of a nucleic acid molecule using repair recombination in a simple one component system. The frequency of the recombination reaction is high, allowing a range of feasible selection strategies to identify successful recombination events. The method involves the steps of bringing a first nucleic acid molecule into contact with a second nucleic acid molecule in the presence of a phage annealing protein into contact with a second nucleic acid molecule in the presence of a phage annealing protein, or a functional equivalent or fragment thereof, wherein said first nucleic acid molecule comprises at least two regions of shared sequence homology with the second nucleic acid molecule, under conditions suitable for repair recombination to occur between said first and second nucleic acid molecules; ad selecting a nucleic acid molecule whose sequence has been altered so as to include sequence from said second nucleic acid molecule.Type: ApplicationFiled: December 22, 2003Publication date: May 27, 2004Inventors: Joep P P Muijrers, Youming Zhang, Andrian Francis Stewart
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Publication number: 20030113837Abstract: The present invention is directed to methods and compositions for TnpI-mediated genetic engineering using the Bacillus thuringiensis recombinase TnpI and the TnpI recombination substrates TRT or TRT′, and variants thereof. In particular, the invention relates to TRT or TRT′ sequences, and variants thereof, vectors, cells and kits useful for TnpI-mediated genetic recombination, as well as methods for the use of TRT or TRT′ sequences for TnpI-mediated genetic engineering.Type: ApplicationFiled: June 30, 2001Publication date: June 19, 2003Inventors: A. Francis Stewart, Youming Zhang, Bernard Hallet
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Publication number: 20030036198Abstract: The invention refers to a novel method for cloning DNA molecules using a homologous recombination mechanism between at least two DNA molecules comprising: a) providing a host cell capable of performing homologous recombination, b) contacting in said host cell a first DNA molecule which is capable of being replicated in said host cell with a second DNA molecule comprising at least two regions of sequence homology to regions on the first DNA molecule, under conditions which favour homologous recombination between said first and second DNA molecules and c) selecting a host cell in which homologous recombination between said first and second DNA molecules has occurred.Type: ApplicationFiled: August 30, 2002Publication date: February 20, 2003Applicant: Europaisches Laboratorium fur Molekularbiologie (EMBL)Inventors: Francis Stewart, Youming Zhang, Frank Buchholz
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Patent number: 6509156Abstract: The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.Type: GrantFiled: June 5, 2000Date of Patent: January 21, 2003Assignee: Europaisches Laboratorium Fur Molekularoiologie (EMBL)Inventors: Francis Stewart, Youming Zhang, Frank Buchholz
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Patent number: 6355412Abstract: The present invention is directed to methods and compositions for DNA subcloning using bacterial recombinase-mediated homologous recombination. The invention relates to methods for cloning, compositions comprising polynucleotides usefall as cloning vectors, cells comprising such polynucleotide compositions, and kits useful for cloning mediated by bacterial recombinases, such as RecE/T and Red&agr;/&bgr;.Type: GrantFiled: July 9, 1999Date of Patent: March 12, 2002Assignee: The European Molecular Biology LaboratoryInventors: A. Francis Stewart, Youming Zhang, Joep Pieter Paul Muyrers