Abstract: The present disclosure provides a pumping device of a vehicle seat and its operation mechanism. The pumping device includes: a housing encasing a pumping mechanism; a spring guide which has a ring shape formed with a cavity and is seated on a top surface of the housing, a coil spring being inserted in the spring guide; and a lever bracket which has a protruding portion formed on a central portion, an operating lever being coupled to a flange, and a locking protrusion protruding from the flange and locked to the coil spring through the guide slit of the spring guide.

Abstract: Provided is an apparatus for adjusting a seat height, including: a fixed member fixedly installed at a seat cushion, the fixed member including a plurality of first stoppers at predetermined radial positions from a center of an axial direction; an input member rotatably installed on an axis concentric with the fixed member, the input member having hook parts formed to protrude at radial positions facing the first stoppers; an output member rotatably and concentrically installed with respect to the input member, the output member having a friction flange part formed to protrude along a circumferential direction at a predetermined radial position facing an inner circumferential surface of the input member; and a frictional rotation means rotatably installed between the inner circumferential surface of the input member and an outer circumferential surface of the friction flange part to be flexibly rotated along the circumferential direction.

Abstract: Provided is an apparatus for adjusting a seat height, including: a fixed member fixedly installed at a seat cushion, the fixed member including a plurality of first stoppers at predetermined radial positions from a center of an axial direction; an input member rotatably installed on an axis concentric with the fixed member, the input member having hook parts formed to protrude at radial positions facing the first stoppers; an output member rotatably and concentrically installed with respect to the input member, the output member having a friction flange part formed to protrude along a circumferential direction at a predetermined radial position facing an inner circumferential surface of the input member; and a frictional rotation means rotatably installed between the inner circumferential surface of the input member and an outer circumferential surface of the friction flange part to be flexibly rotated along the circumferential direction.

Abstract: A pharmaceutical composition for preventing or treating a fracture or osteoporosis, includes, as an active ingredient, a gene selected from a group consisting of slit1, slit2, slit3, robo1, robo2 and vilse, or an expressed protein of the gene. A marker composition for predicting the risk of the occurrence of a fracture or osteoporosis includes the protein. A kit for predicting the risk of the occurrence of a fracture or osteoporosis includes an antibody that specifically binds to the protein. An information provision method for predicting the risk of the occurrence of a fracture or osteoporosis includes measuring the level of expression of the slit protein through an antigen-antibody binding reaction using an antibody that specifically binds to the protein. The slit3 may increase bone formation and decrease bone reabsorption in a cellular and animal model, and has a negative correlation with the incidence rate of osteoporosis.

Abstract: A fusion protein that includes a polypeptide binding specifically to a constant region of an antibody and a stabilization protein linked to a terminus of the polypeptide, a polynucleotide encoding the fusion protein, a cell including the polynucleotide, a method of preparing the fusion protein, and a method of isolating an antibody by using the fusion protein.

Abstract: The present invention relates to a reclining apparatus for a rear seat of a vehicle, having a back frame having a upper end and a lower end; a cushion frame having a front side and a back side, wherein the back side of the cushion frame is pivotally connected to the lower end of the back frame; a back frame locking module disposed on the upper end of the back frame, the back frame locking module removably engageable to a body panel and acting as a pivot center when engaged with the body panel; and a guide module configured to guide the cushion frame pivotally connected to the back frame so that the lower portion of the back frame pivots about the upper portion when the cushion frame slides up forward or down rearward to allow the back frame to move between an upright position and a reclined position.

Abstract: A fluid reaction device includes a microfluidic reaction chip which accommodates a fluid, a heater, and a heat transfer facilitating layer which is interposed between the microfluidic reaction chip and the heater, the heat transfer facilitating layer has a higher thermal conductivity than air and can hold particles, wherein formation of an air layer can be prevented.

Abstract: The present invention relates to a reclining apparatus for a rear seat of a vehicle, having a back frame having a upper end and a lower end; a cushion frame having a front side and a back side, wherein the back side of the cushion frame is pivotally connected to the lower end of the back frame; a back frame locking module disposed on the upper end of the back frame, the back frame locking module removably engageable to a body panel and acting as a pivot center when engaged with the body panel; and a guide module configured to guide the cushion frame pivotally connected to the back frame so that the lower portion of the back frame pivots about the upper portion when the cushion frame slides up forward or down rearward to allow the back frame to move between an upright position and a reclined position.

Abstract: Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

Abstract: Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

Abstract: A fusion protein that includes a polypeptide binding specifically to a constant region of an antibody and a stabilization protein linked to a terminus of the polypeptide, a polynucleotide encoding the fusion protein, a cell including the polynucleotide, a method of preparing the fusion protein, and a method of isolating an antibody by using the fusion protein.

Abstract: Provided is a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase, (d) an endonuclease, (e) a DNA polymerase, and (f) a primer.

Abstract: A method of controlling a mobile terminal, and which includes displaying, on a touch screen display of the mobile terminal, an operation screen corresponding to a current operating mode executing on the mobile terminal; detecting, via a detecting device, a touch input on the touch screen display of the mobile terminal; determining, via a controller on the mobile terminal, a first finger characteristic describing a finger touching the touch screen display and a second finger characteristic describing the finger touching the touch screen display that is different than the first finger characteristic; performing, via the controller, a first operation relevant to the current operating mode based on the determined first finger characteristic; and performing, via the controller, a second operation relevant to the current operating mode based on the determined second finger characteristic.

Abstract: Disclosed is a method of determining an initial concentration of a target nucleic acid within a sample using real-time nucleic acid amplification data. Amplification efficiencies of the target nucleic acid with respect to amplification time are obtained from signals of amplified products, and an amplification efficiency function with respect to amplification time is formulated employing the amplification efficiencies.

Abstract: Provided is a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase, (d) an endonuclease, (e) a DNA polymerase, and (f) a primer.

Abstract: Provided is a method for quantifying an initial concentration of a nucleic acid from a real-time nucleic acid amplification data. Nucleic acid (DNA or RNA) extracted from organism or virus is amplified using an enzyme. Then, the initial concentration of the nucleic acid is found by calculating the characteristic amplification cycle number or the characteristic amplification time at which the fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid has half of its maximum value, or the characteristic amplification cycle number or the characteristic amplification time at which the amplification efficiency has the maximum or the minimum value, or the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid. Accordingly, the initial concentration of the nucleic acid can be calculated without differentiation or integration.

Abstract: Provided is kit for and a method of amplifying a nucleic acid using rolling cyclic amplification (RCA), including amplifying a nucleic acid together with formation of a single-strand circular DNA template using RCA by reacting a reaction solution including: (a) two hairpin oligos, (b) a target nucleic acid, (c) a DNA ligase,(d) an endonuclease, (e) a DNA polymerase, and (f) a primer.

Abstract: A transmembrane fusion protein including ubiquitin or a ubiquitin-like protein, a membrane translocation sequence linked to the C-terminus of the ubiquitin or ubiquitin-like protein, and a biologically active molecule linked to the C-terminus of the membrane translocation sequence is disclosed herein. A polynucleotide encoding the transmembrane fusion protein, a recombinant expression vector including the polynucleotide sequence, a cell transformed by the recombinant expression vector, and a method of delivering the biologically active molecule into a cell using the transmembrane fusion protein are also disclosed.

Abstract: Provided are a centrifugal force based microfluidic device which can automatically perform a dilution operation and a microfluidic system including the same. The centrifugal force based microfluidic device for dilution includes a rotatable disk type platform, a mixing chamber disposed on the platform; a buffer solution storage disposed on a portion of the platform which is closer to a center of the platform than the mixing chamber, connected to the mixing chamber through a channel to supply a predetermined amount of buffer solution to the mixing chamber at least one time, and a plurality of diluted solution chambers which are disposed on a portion of the platform which is farther from the center of the platform than the mixing chamber, are each connected to the mixing chamber through flow paths extended from a middle exit corresponding to a predetermined water level, and sequentially receiving a solution which is serially diluted in the mixing chamber at least one time.

Abstract: A method of reducing a temperature difference between a high-temperature and a low-temperature substrate includes interposing a heat transfer facilitating layer which has a higher thermal conductivity than air and can hold particles between the substrates, and maintaining close contact between the high-temperature substrate, the heat transfer facilitating layer, and the low-temperature substrate, wherein formation of an air layer can be at least substantially prevented between the high-temperature substrate and the heat transfer facilitating layer, and between the low-temperature substrate and the heat transfer facilitating layer. A fluid reaction device includes a microfluidic reaction chip which accommodates a fluid, a heater, and a heat transfer facilitating layer which is interposed between the microfluidic reaction chip and the heater, the heat transfer facilitating layer has a higher thermal conductivity than air and can hold particles, wherein formation of an air layer can be prevented.