Abstract: The present disclosure provides a method and apparatus for biomolecular multiplexed imaging through the iterative unmixing of fluorophores. According to the first embodiments of the present disclosure, although signals of two fluorophores are detected in the first fluorescent detection spectral range, the signals of the two images obtained from two detection spectral ranges can be unmixed through the iterative minimization of mutual information. Furthermore, the present disclosure provides a multi-color unmixing method and apparatus through the iterative minimization of mutual information. In the second embodiments of the present disclosure, a plurality of images of a plurality of fluorophores marking different biomolecules, respectively, for example, N fluorophores are obtained. Images, each containing the signals of single fluorophore can be obtained from the obtained images while minimizing the mutual information shared by images of each of pairs each consisting of two of the obtained images.

Abstract: Provided are a rubber latex used as a substrate for an impact modifier, a preparation method thereof, and an impact modifier prepared using the rubber latex. The rubber latex includes a rubber monomer as a main component and has a decreasing gel content from a core to a shell(s). The preparation method includes polymerizing a core followed by polymerization of a shell(s) has a lower gel content that the core. The impact modifier is prepared by common graft polymerization using the rubber latex as a substrate. The rubber latex has a high gel content core and a low gel content shell (s), and thus, is free from problems involved in low or high gel content rubber particles. The rubber latex can be used as a substrate for a high efficiency impact modifier with high rubber content and enhanced impact strength and processability.