Abstract: Disclosed herein are a transformed Synechococcus elongatus strain having improved capability of producing acetone and a method for producing acetone and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce acetone with high selectivity using carbon dioxide as a carbon source. The present disclosure is economical because the Synechococcus elongatus strain can economically produce high value-added acetone using carbon dioxide existing in the atmosphere as a carbon source without requiring an additional catalytic reaction. Also, the present disclosure is environment-friendly because carbon dioxide in the atmosphere can be removed or reduced using the microorganism.

Abstract: Provided is a method for preparing a lignocellulosic biomass hydrolysate having toxicity reduced or removed and being used for fermentation by microorganisms, comprising the steps of: pretreating, hydrolyzing, and using an electrochemical detoxification method.

Abstract: The present disclosure discloses a transformed Synechococcus elongatus strain capable of producing biodiesel directly from carbon dioxide and a method for producing biodiesel and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce biodiesel in large scale using carbon dioxide as a carbon source. The Synechococcus elongatus strain is environment-friendly because it can be used to remove or reduce carbon dioxide in the atmosphere. The strain of the present disclosure is advantageous in that it can produce biodiesel in large scale because it grows faster and exhibits excellent carbon dioxide fixation capability as compared to other photosynthetic microorganisms.

Abstract: The present disclosure relates to a novel strain capable of saccharifying and fermenting biomass-derived cellulose and a recombinant strain thereof with improved biomass saccharification capability. The present disclosure also relates to a method for producing a material useful as a bioenergy source material such as ethanol, acetic acid, formic acid, etc. using the strain or the recombinant strain. The strain or the recombinant strain may be usefully used in bioenergy industry.

Abstract: Disclosed herein are an expression vector capable of expressing myrcene, an Escherichia coli strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed Escherichia coli strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the Escherichia coli strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.

Abstract: The present disclosure relates to a hydrolysate of a mixture of lignocellulosic biomass and seaweed biomass. By mixing seaweed biomass with lignocellulosic biomass and then preparing a hydrolysate, lignocellulosic biomass-derived acetic acid is consumed together with seaweed biomass-derived mannitol. As a result, high sugar productivity can be maintained while reducing fermentation inhibitors. Because the present disclosure can solve the problem of lignocellulosic biomass of decreased fermentation efficiency due to lignocellulose-derived fermentation inhibitors and the problem of seaweed biomass of very low productivity in spite of long fermentation time, the hydrolysate according to the present disclosure may be used to produce biofuels and biochemicals economically.

Abstract: The present disclosure relates to a novel strain capable of saccharifying and fermenting biomass-derived cellulose and a recombinant strain thereof with improved biomass saccharification capability. The present disclosure also relates to a method for producing a material useful as a bioenergy source material such as ethanol, acetic acid, formic acid, etc. using the strain or the recombinant strain. The strain or the recombinant strain may be usefully used in bioenergy industry.

Abstract: Disclosed is a shuttle vector that can be used for Corynebacterium and E. coli, containing: a repressor selected from a group consisting of a lacI repressor and a tetR repressor; a promoter selected from a group consisting of a trc promoter, a tetA promoter and a LacUV5 promoter; a replication origin pBL1 derived from Corynebacterium glutamicum; and a replication origin ColE1 of E. coli. A host cell transformed with the shuttle vector can effectively produce industrially useful substances. Also, the shuttle vector may be used to easily insert various combinations of target genes and, as a result, a variety of vectors can be prepared effectively.

Abstract: Disclosed herein are a transformed Synechococcus elongatus strain having improved capability of producing acetone and a method for producing acetone and a method for removing carbon dioxide using the same. In an aspect, the transformed Synechococcus elongatus strain of the present disclosure can produce acetone with high selectivity using carbon dioxide as a carbon source. The present disclosure is economical because the Synechococcus elongatus strain can economically produce high value-added acetone using carbon dioxide existing in the atmosphere as a carbon source without requiring an additional catalytic reaction. Also, the present disclosure is environment-friendly because carbon dioxide in the atmosphere can be removed or reduced using the microorganism.

Abstract: The present disclosure discloses a vector that can be used for both cyanobacteria and E. coli, which contains, sequentially, a pUC replication origin as a replication origin; a spectinomycin-resistant gene as a selection marker; and a promoter selected from a group consisting of a trc promoter, a tetA promoter or a modified tetA promoter, a BAD promoter and a cbbL promoter. An industrially useful substance may be produced effectively using a host cell transformed with the vector. Also, the vector may be used to insert a variety of target genes through simple combination and, as a result, various vectors can be prepared effectively.

Abstract: A method for isolating a highly furfural-resistant strain is disclosed. The method includes (A) mixing soil with an isotonic solution and collecting the soil supernatant from the mixture, (B) diluting the soil supernatant and spreading the dilution on a medium supplemented with carboxymethyl cellulose as a nutrient source, and (C) growing a desired strain by culture in the medium spread with the diluted soil supernatant and isolating the strain. Also disclosed is a strain isolated by the method. The strain is Enterobacter cloacae strain GGT036 [Accession No. KCTC 12672BP].

Abstract: The present disclosure relates to a method for simultaneously degrading lignin and cellulose and for boosting effect on the cellulase activity using a specific catalyst. Since the present disclosure allows for the preparation of sugars by degrading not only lignin but also cellulose and hemicellulose using the enzymes which were previously known only as lignin-degrading biocatalysts, it provides the advantage that the preparation of a hydrolysate as a source material for the production of biofuels or biochemicals from lignocellulosic biomass can be simplified and facilitated. As a result, the present disclosure can reduce enzyme cost and can provide improved production efficiency by simplifying the biofuel production process.

Abstract: Disclosed is a shuttle vector that can be used for Corynebacterium and E. coli, containing: a repressor selected from a group consisting of a lacI repressor and a tetR repressor; a promoter selected from a group consisting of a trc promoter, a tetA promoter and a LacUV5 promoter; a replication origin pBL1 derived from Corynebacterium glutamicum; and a replication origin ColE1 of E. coli. A host cell transformed with the shuttle vector can effectively produce industrially useful substances. Also, the shuttle vector may be used to easily insert various combinations of target genes and, as a result, a variety of vectors can be prepared effectively.

Abstract: Provided is a method for producing a saccharified solution of wood-based biomass having toxicity reduced or removed, comprising the steps of: preparing a saccharified solution in which wood-based biomass has been pretreated by hydrolysis; and reducing toxicity in the saccharified solution using an electrochemical detoxification method. The method is capable of efficiently removing the toxicity of compounds which are produced during the pretreatment process and inhibit growth of microorganisms and fermentation. In addition, the method is capable of increasing the efficiency of production by minimizing sugar loss and additional costs during the toxicity removal process.

Abstract: The present disclosure relates to a strain capable of producing succinate using starch accumulated in microalgae which grow using carbon dioxide as a direct carbon source without converting it to glucose and a method for producing succinate using the same. The present disclosure provides a strain producing succinate from carbon dioxide, selected from a group consisting of Corynebacterium glutamicum BL-1-pBlAmyS (KCTC 12585BP) and Corynebacterium glutamicum BL-1-pSbAmyA (KCTC 12587BP). The present disclosure also provides a method for producing succinate from carbon dioxide, including fermenting starch by inoculating the strain producing succinate from carbon dioxide in a starch-containing medium.

Abstract: The furfural-resistant strain containing the furfural-resistant gene according to the present disclosure may be effectively grown in a furfural-containing medium. Accordingly, the problem that microorganism fermentation was difficult because toxic by-products such as furfural are contained in a hydrolysate derived from inedible lignocellulosic biomass may be solved. Further, according to the method for producing a strain of the present disclosure, the resistant gene may be selected from relatively small number of target genes. Thus, time, cost and the like for developing the resistant strain may be saved. Further, this method for identifying genes may be broadly applied to methods for identifying various unknown functional genes in addition to the furfural-resistant gene.

Abstract: Provided is a method for producing alcohol or hydrogen gas comprising culturing alcohol-producing microorganisms in a waste fermented solution generated from the bioethanol production process. Glycerol included in the waste fermented solution generated from the bioethanol production process is converted by the microorganisms to butanol under an anaerobic condition. Since the waste fermented solution generated from the bioethanol production process can be utilized as a source of a biofuel, environmental and energy problems can be solved at once.

Abstract: Provided is a method for producing alcohol or hydrogen gas comprising culturing alcohol-producing microorganisms in a waste fermented solution generated from the bioethanol production process. Glycerol included in the waste fermented solution generated from the bioethanol production process is converted by the microorganisms to butanol under an anaerobic condition. Since the waste fermented solution generated from the bioethanol production process can be utilized as a source of a biofuel, environmental and energy problems can be solved at once.