Patents by Inventor Yousong Ding
Yousong Ding has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11970689Abstract: The present invention relates to recombinant cyanobacterial cells for the production of a chemical compound of interest. In particular, the present invention relates to genetic modifications that introduce one or more heterologous phosphopantetheinyl transferases (PPTases) into a cyanobacterial cell. These cells can, optionally, further comprise heterologous carrier protein and nucleic acid constructs that provide the cyanobacterial cells with the capability of producing chemicals of interest or compounds of interest, such secondary metabolites polyketides, nonribosomal peptides and their hybrids, the three major families of bioactive natural products, of cyanobacteria and other bacterial phyla, secondary metabolites analogs, and unnatural compounds.Type: GrantFiled: April 25, 2022Date of Patent: April 30, 2024Assignee: University of Florida Research Foundation, IncorporatedInventors: Yousong Ding, Guang Yang
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Patent number: 11879144Abstract: The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.Type: GrantFiled: January 10, 2023Date of Patent: January 23, 2024Assignee: University of Florida Research Foundation, IncorporatedInventors: Yousong Ding, Ran Zuo
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Publication number: 20230348929Abstract: The present disclosure relates to compositions comprising nitroreductase enzymes; plants, plant calluses, plant seeds, and vegetables incorporating genes expressing nitroreductase enzymes; methods for stably and operably incorporating genes expressing nitroreductase enzymes into plants and plant tissues; wherein the nitroreductase enzymes are capable of reducing nitro groups on phytotoxic and/or otherwise harmful compounds such as, for example, thaxtomins, wherein the thaxtomins are secreted by plant pathogenic bacteria and/or exogenously applied as an agricultural composition such as an herbicide, and wherein reducing the nitro groups renders the thaxtomins non-damaging or reduces the level of damage from the thaxtomins to the plants expressing the nitroreductase enzymes.Type: ApplicationFiled: December 10, 2020Publication date: November 2, 2023Inventors: Yousong DING, Guangde JIANG
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Publication number: 20230287466Abstract: The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.Type: ApplicationFiled: January 10, 2023Publication date: September 14, 2023Applicant: UNIVERSITY OF FLORIDA RESEARCHInventors: Yousong Ding, Ran Zuo
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Publication number: 20230272019Abstract: The subject invention pertains to compositions and methods for preparing and using recombinant proteins based on the fungal Coprinus comatus Y3 protein to control plant and animal viruses and microbes, and diagnose, prevent and treat cancers. Methods are disclosed using compositions comprising recombinant Y3 proteins to diagnose, prevent and/or treat cancer diseases based on recombinant Y3 protein interaction with glycans expressed on cancer cells.Type: ApplicationFiled: October 28, 2022Publication date: August 31, 2023Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATEDInventors: Yousong Ding, Peilan Zhang, Steven Douglas Bruner
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Patent number: 11655477Abstract: The present disclosure includes genetically engineered, non-pathogenic Streptomyces bacterium with exogenous, non-native Thaxtomin A (ThxA) biosynthetic gene clusters conferring the genetically engineered, non-pathogenic Streptomyces bacterium with the ability to produce thaxtomin A. Also included are methods of providing thaxtomin producing capability in non-native Streptomyces bacterial strains, methods of producing thaxtomin compounds with the genetically engineered Streptomyces bacteria of the present disclosure, and methods of producing thaxtomin compounds and nitro-tryptophan analogs, and fluorinated thaxtomin compounds, analogs, and intermediates with the genetically engineered Streptomyces bacteria of the present disclosure.Type: GrantFiled: May 23, 2018Date of Patent: May 23, 2023Assignee: University of Florida Research Foundation, IncorporatedInventors: Rosemary Loria, Yucheng Zhang, Yousong Ding, Guangde Jiang
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Patent number: 11591627Abstract: The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.Type: GrantFiled: March 21, 2019Date of Patent: February 28, 2023Assignee: University of Florida Research Foundation, IncorporatedInventors: Yousong Ding, Ran Zuo
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Patent number: 11530415Abstract: The present disclosure includes refactored thaxtomin biosynthetic gene clusters including thaxtomin modules including one or more thaxtomin genes such that the expression of the refactored thaxtomin biosynthetic gene cluster produces at least one thaxtomin compound in the absence of thaxtomin-inducing conditions. Also included are genetically engineered Streptomyces bacterium from a non-pathogenic Streptomyces strain comprising an exogenous, refactored thaxtomin biosynthetic gene cluster of the present disclosure, such that the expression of the refactored thaxtomin biosynthetic gene cluster provides the genetically engineered Streptomyces bacterium with the ability to produce at least one thaxtomin compound in the absence of thaxtomin-inducing conditions. The present disclosure also includes methods of producing thaxtomin compounds, analogs, or intermediate with the refactored thaxtomin biosynthetic gene clusters and genetically engineered bacteria of the present disclosure.Type: GrantFiled: November 29, 2018Date of Patent: December 20, 2022Assignee: University of Florida Research Foundation, IncorporatedInventors: Yousong Ding, Guangde Jiang
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Patent number: 11512119Abstract: The subject invention pertains to compositions and methods for preparing and using recombinant proteins based on the fungal Coprinus comatus Y3 protein to control plant and animal viruses and microbes, and diagnose, prevent and treat cancers. Methods are disclosed using compositions comprising recombinant Y3 proteins to diagnose, prevent and/or treat cancer diseases based on recombinant Y3 protein interaction with glycans expressed on cancer cells.Type: GrantFiled: July 26, 2018Date of Patent: November 29, 2022Assignee: University of Florida Research Foundation, IncorporatedInventors: Yousong Ding, Peilan Zhang, Steven Douglas Bruner
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Publication number: 20220372427Abstract: The present invention relates to recombinant cyanobacterial cells for the production of a chemical compound of interest. In particular, the present invention relates to genetic modifications that introduce one or more heterologous phosphopantetheinyl transferases (PPTases) into a cyanobacterial cell. These cells can, optionally, further comprise heterologous carrier protein and nucleic acid constructs that provide the cyanobacterial cells with the capability of producing chemicals of interest or compounds of interest, such secondary metabolites polyketides, nonribosomal peptides and their hybrids, the three major families of bioactive natural products, of cyanobacteria and other bacterial phyla, secondary metabolites analogs, and unnatural compounds.Type: ApplicationFiled: April 25, 2022Publication date: November 24, 2022Applicant: University of Florida Research Foundation, IncorporatedInventors: Yousong Ding, Guang Yang
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Publication number: 20220227820Abstract: Aspects of the disclosure relate to compositions and methods for expressing glycan binding proteins (GBPs) in a cell or cells. In some embodiments, the disclosure provides recombinant Ab-Y3 protein variants and isolated nucleic acids engineered to express the same. In some embodiments, the disclosure provides methods of isolating, purifying, detecting, or screening for certain proteins (e.g., IgG proteins) using recombinant Ab-Y3 protein variants.Type: ApplicationFiled: April 23, 2020Publication date: July 21, 2022Applicant: University of Florida Research Foundation, IncorporatedInventors: Yousong Ding, Steven Douglas Bruner, Peilan Zhang, Brian MacTavish
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Patent number: 11352601Abstract: The present invention relates to recombinant cyanobacterial cells for the production of a chemical compound of interest. In particular, the present invention relates to genetic modifications that introduce one or more heterologous phosphopantetheinyl transferases (PPTases) into a cyanobacterial cell. These cells can, optionally, further comprise heterologous carrier protein and nucleic acid constructs that provide the cyanobacterial cells with the capability of producing chemicals of interest or compounds of interest, such secondary metabolites polyketides, nonribosomal peptides and their hybrids, the three major families of bioactive natural products, of cyanobacteria and other bacterial phyla, secondary metabolites analogs, and unnatural compounds.Type: GrantFiled: July 27, 2018Date of Patent: June 7, 2022Assignee: University of Florida Research Foundation, IncorporatedInventors: Yousong Ding, Guang Yang
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Patent number: 11078467Abstract: The disclosure relates to the field of fusion proteins. In some aspects, the disclosure relates to artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes and uses thereof. In some aspects, the disclosure relates to corn-pounds produced by artificial cytochrome P450 enzymes.Type: GrantFiled: October 26, 2017Date of Patent: August 3, 2021Assignee: University of Florida Research Foundation, IncorporatedInventors: Yousong Ding, Ran Zuo
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Publication number: 20210163451Abstract: Thaxtomins are phytotoxic secondary metabolites produced in plant pathogenic Streptomyces strains and have received considerable interests as bioherbicides. A cell-free, biocombinatorial approach was developed to produce a thaxtomin library for herbicide development. Combination of biosynthetic enzymes led to the production of 136 substituted 2,5-diketopiperazines, thaxtomin D, thaxtomin B and thaxtomin A analogs in a single pot. Furthermore, rational engineering of TxtA allowed the synthesis of azido-containing thaxtomin analog. Selected unnatural thaxtomins demonstrated improved herbicidal activities. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.Type: ApplicationFiled: May 16, 2019Publication date: June 3, 2021Inventors: Yousong Ding, Guangde Jiang
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Publication number: 20210108237Abstract: The disclosure relates, in some aspects, to compositions and methods useful for production of nitrated aromatic molecules. The disclosure is based, in part, on whole cell systems expressing artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes. In some aspects, the disclosure relates to methods of producing nitrated aromatic molecules in whole cell systems having artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes.Type: ApplicationFiled: March 21, 2019Publication date: April 15, 2021Applicant: University of Florida Research Foundation, IncorporatedInventors: Yousong Ding, Ran Zuo
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Publication number: 20210094988Abstract: The subject invention pertains to compositions and methods for preparing and using recombinant proteins based on the fungal Coprinus comatus Y3 protein to control plant and animal viruses and microbes, and diagnose, prevent and treat cancers. Methods are disclosed using compositions comprising recombinant Y3 proteins to diagnose, prevent and/or treat cancer diseases based on recombinant Y3 protein interaction with glycans expressed on cancer cells.Type: ApplicationFiled: July 26, 2018Publication date: April 1, 2021Inventors: Yousong Ding, Peilan Zhang, Steven Douglas Bruner
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Publication number: 20210087522Abstract: The present invention relates to recombinant cyanobacterial cells for the production of a chemical compound of interest. In particular, the present invention relates to genetic modifications that introduce one or more heterologous phosphopantetheinyl transferases (PPTases) into a cyanobacterial cell. These cells can, optionally, further comprise heterologous carrier protein and nucleic acid constructs that provide the cyanobacterial cells with the capability of producing chemicals of interest or compounds of interest, such secondary metabolites polyketides, nonribosomal peptides and their hybrids, the three major families of bioactive natural products, of cyanobacteria and other bacterial phyla, secondary metabolites analogs, and unnatural compounds.Type: ApplicationFiled: July 27, 2018Publication date: March 25, 2021Inventors: Yousong DING, Guang YANG
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Publication number: 20200362298Abstract: The present disclosure includes refactored thaxtomin biosynthetic gene clusters including thaxtomin modules including one or more thaxtomin genes such that the expression of the refactored thaxtomin biosynthetic gene cluster produces at least one thaxtomin compound in the absence of thaxtomin-inducing conditions. Also included are genetically engineered Streptomyces bacterium from a non-pathogenic Streptomyces strain comprising an exogenous, refactored thaxtomin biosynthetic gene cluster of the present disclosure, such that the expression of the refactored thaxtomin biosynthetic gene cluster provides the genetically engineered Streptomyces bacterium with the ability to produce at least one thaxtomin compound in the absence of thaxtomin-inducing conditions. The present disclosure also includes methods of producing thaxtomin compounds, analogs, or intermediate with the refactored thaxtomin biosynthetic gene clusters and genetically engineered bacteria of the present disclosure.Type: ApplicationFiled: November 29, 2018Publication date: November 19, 2020Inventors: Yousong DING, Guangde JIANG
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Publication number: 20200140905Abstract: The present disclosure includes genetically engineered, non-pathogenic Streptomyces bacterium with exogenous, non-native Thaxtomin A (ThxA) biosynthetic gene clusters conferring the genetically engineered, non-pathogenic Streptomyces bacterium with the ability to produce thaxtomin A. Also included are methods of providing thaxtomin producing capability in non-native Streptomyces bacterial strains, methods of producing thaxtomin compounds with the genetically engineered Streptomyces bacteria of the present disclosure, and methods of producing thaxtomin compounds and nitro-tryptophan analogs, and fluorinated thaxtomin compounds, analogs, and intermediates with the genetically engineered Streptomyces bacteria of the present disclosure.Type: ApplicationFiled: May 23, 2018Publication date: May 7, 2020Inventors: Rosemary LORIA, Yucheng ZHANG, Yousong DING, Guangde JIANG
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Patent number: 10550079Abstract: The disclosure relates to the field of fusion proteins. In some aspects, the invention to artificial fusion proteins comprising cytochrome P450 enzymes linked to reductase enzymes and uses thereof. In some aspects, the disclosures relates to compounds produced by artificial cytochrome P450 enzymes.Type: GrantFiled: October 30, 2018Date of Patent: February 4, 2020Assignee: University of Florida Research Foundation, IncorporatedInventor: Yousong Ding